We narrowed to 4,751 results for: MLS

1. Anti-Pan-Synapsin [L125/129R]

   Plasmid

   #177472

   Purpose

   Mammalian Expression Plasmid of anti-Pan-Synapsin (Human). Derived from hybridoma L125/129.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-Pan-Synapsin (Homo sapiens) recombinant mouse monoclonal antibody
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Nov. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTT3-SP-6xHis-NTRK1(FL)-FLAG

   Plasmid

   #157620

   Purpose

   Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   NTRK1 (NTRK1 Human)
   

   Use

   Tags

   6xHis and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAVS1_gRNA

   Plasmid

   #196139

   Purpose

   gRNA targeting the AAVS1 locus in a third generation Cas9 backbone with GFP

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   AAVS1 gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pD649-HAsp-SPACA6-COMP5AP-AviTag-9xHis

   Plasmid

   #157274

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   SPACA6 (SPACA6 Human)
   

   Use

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJFA334E9

   Plasmid

   #49237

   Purpose

   TET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cells

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   TET1 tet methylcytosine dioxygenase 1 (TET1 Human, Synthetic)
   

   Use

   TALEN; Tale-tet1 expression vector

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac1_GI.1_N116C-G193C

   Plasmid

   #162580

   Purpose

   Expresses norovirus GI.1 VP1 protein with mutations N116C-G193C in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Asparagine 116 was mutated to Cysteine and Glycin…

   Promoter

   polyhedrin

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWZLblasti-HA-ERK2 GOF

   Plasmid

   #53174

   Purpose

   Expresses HA tagged human ERK2 R67SD321N from blasticidin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   ERK2 (MAPK1 Human, Mustard Weed)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   R67S/D321N

   Promoter

   Available since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

8. Chr8q_Centromere-Targeting_gRNA

   Plasmid

   #195128

   Purpose

   gRNA targeting centromere-proximal location on Chromosome 8q in a third generation Cas9 backbone with GFP

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   Chr8q gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. MDH1-miR-31-PGK-GFP-Blast

   Plasmid

   #31515

   Depositor

   Chang-Zheng Chen

   Article

   Arnold et al Genome Res. 2011 May . 21(5):798-810.

   Insert

   miR-31 (Mir31 Mouse)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

10. Strep-HA-VCF1 WT

    Plasmid

    #223020

    Purpose

    Expression of Strep-HA-tagged VCF1 in mammalian cells.

    Depositor

    Niels Mailand

    Article

    Mirsanaye et al Nat Commun. 2024 Mar 19;15(1):2459. doi:

    Insert

    VCF1 (VCF1 Human)
    

    Use

    Tags

    Strep-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO2

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. fck-Jaws-GFP-ER2

    Plasmid

    #65013

    Purpose

    Lentivirus-mediated expression of Jaws-ER2 under the CaMKII promoter.

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws-GFP-ER2
    

    Use

    Lentiviral

    Tags

    ER2 and GFP

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    CaMKII

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. Rosa26_gRNA

    Plasmid

    #196138

    Purpose

    gRNA targeting the Rosa26 locus in a thid generation Cas9 backbone with TagBFP2

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    Rosa26 gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pD649-HAsp-THY1-COMP5AP-AviTag-9xHis

    Plasmid

    #157056

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    THY1 (THY1 Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. APEX2-6xHp

    Plasmid

    #214411

    Purpose

    Lipid droplet localization of APEX2-6xHp for electron microscopy

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    6x Spastin aa 43-92 (APEX2 Human)
    

    Use

    Tags

    APEX2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lentiviral click editor (CE1) plasmid - LTR-pEF1a-PCV2-nCas9-EcKlenow (DRR921)

    Plasmid

    #217798

    Purpose

    Lentiviral plasmid to produce the prototypical CE1 construct (PCV2-nCas9-EcKlenow), expressed from the EF1a promoter

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR and Lentiviral

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    EF1a

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Control click editor - pCMV-T7-deadPCV2-nCas9-EcKlenow (EMK492)

    Plasmid

    #208943

    Purpose

    A control CE1 construct with catalytically attenuated PCV2 HUH, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    dPCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    PCV2(Y96F); nSpCas9(H840A); EcKlenow(-exo;D355A/E…

    Promoter

    CMV and T7

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Anti-NALCN [N185/7R]

    Plasmid

    #177508

    Purpose

    Mammalian Expression Plasmid of anti-NALCN (Rat). Derived from hybridoma N185/7.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-NALCN (Rattus norvegicus) recombinant mouse monoclonal antibody (Nalcn Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-B103 (LM2963)

    Plasmid

    #208960

    Purpose

    A variant CE1 construct with B103 DNA polymerase, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-B103-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-PolBeta (JO574)

    Plasmid

    #208953

    Purpose

    A variant CE1 construct with human DNA polymerase beta, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-PolBeta-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-TaqStoffel (JO595)

    Plasmid

    #208952

    Purpose

    A variant CE1 construct with Taq Stoffel DNA polymerase, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-TaqStoffel-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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