Addgene

We narrowed to 8,996 results for: sacs

Showing: 1521 - 1540 of 8996 results

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pAD01 S. cerevisiae telomerase RNA KBS

Plasmid

#61890

Purpose

Plasmid for run-off in vitro transcription of Saccharomyces cerevisiae KBS RNA

Depositor

Thomas Cech

Article

Dalby et al RNA. 2013 Jun;19(6):841-51. doi: 10.1261

Insert

Telomerase RNA hairpin

Use

Tags

Expression

Mutation

Promoter

T7

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
Filamin-M-stFRET

Plasmid

#61105

Purpose

Expression of Filamin with internal stFRET inserted to measure mechanical stress in real time

Depositor

Fred Sachs

Article

Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

Insert

filamin-stFRET

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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Actinin-M-stFRET

Plasmid

#61103

Purpose

Expression of α-actinin with internal stFRET inserted to measure mechanical stress in real time

Depositor

Fred Sachs

Article

Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

Insert

α-actinin-stFRET

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2015

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046479

Plasmid

#145495

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp4

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp4 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable thrombin;3xFLAG

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046323

Plasmid

#149100

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp3

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp3 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable thrombin;MBP

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046348

Plasmid

#149108

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp6

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp6 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable thrombin;3xFLAG

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pOPTM p62 PB1 ZZ (1-167)

Plasmid

#210429

Purpose

Bacterial expression of MBP-PB1 ZZ (1-167) with TEV cleavage site

Depositor

Carsten Sachse

Article

Tarafder et al Methods Mol Biol. 2019;1880:3-15. doi: 1

Insert

p62 PB1 ZZ (1-167) (NUP62 Human)

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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Actinin-C-stFRET

Plasmid

#61104

Purpose

Expression of α-actinin with C-term stFRET inserted to measure mechanical stress in real time

Depositor

Fred Sachs

Article

Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

Insert

α-actinin-stFRET

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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pa-G-GECO1.2

Plasmid

#67622

Purpose

photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

pa-G-GECO1.2

Use

Tags

Expression

Mammalian

Mutation

V81H, M84K, L189F, T190S

Promoter

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only
pGBW-m4046662

Plasmid

#149221

Purpose

Yeast Expression plasmid for SARS-CoV-2 ORF3a protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF3a protein (ORF3a Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pTEF1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046390

Plasmid

#148996

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp3

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp3 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable thrombin;3xFLAG

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pTEF1

Available since

May 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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BASIC_SEVA_5a6.10

Plasmid

#178934

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Article

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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SIVURA MCP-GFPenvy

Plasmid

#189940

Purpose

Single-integration vector for integration of MCP-GFPEnvy at the URA3 locus in Saccharomyces cerevisiae

Depositor

Tineke Lenstra

Article

Brouwer et al Methods Mol Biol. 2024;2694:235-265. doi

Insert

MCP-GFPEnvy

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046207

Plasmid

#145559

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp6

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp6 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046426

Plasmid

#149140

Purpose

Yeast Expression plasmid for SARS-CoV-2 ORF7b

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF7b (ORF7b Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable thrombin;3xFLAG

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pTEF1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046339

Plasmid

#145565

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp8

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp8 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046441

Plasmid

#145556

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp2

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp2 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046165

Plasmid

#145540

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp7

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp7 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046448

Plasmid

#145426

Purpose

Yeast Expression plasmid for SARS-CoV-2 ORF7b

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF7b (ORF7b Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046445

Plasmid

#145493

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp9

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp9 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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