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CLTA-mCherry HDRT Source (pTR 177)

Plasmid

#112017

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with mCherry

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CLTA-mCherry HDRT (CLTA Human)

Use

CRISPR and Synthetic Biology

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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BATF-GFP HDRT Source (pTR 146)

Plasmid

#112015

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human BATF gene with GFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

BATF-GFP HDRT (BATF Human)

Use

CRISPR and Synthetic Biology

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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RAB11A-BFP HDRT Source (pTR 145)

Plasmid

#112014

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with BFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

RAB11A-BFP HDRT (RAB11A Human)

Use

CRISPR and Synthetic Biology

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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CD4-C-BFP HDRT Source (pTR 176)

Plasmid

#112020

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with BFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CD4-BFP HDRT (CD4 Human)

Use

CRISPR and Synthetic Biology

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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CD4-C-mCherry HDRT Source (pTR 175)

Plasmid

#112019

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with mCherry

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CD4-mCherry HDRT (CD4 Human)

Use

CRISPR and Synthetic Biology

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pIRES-mCerulean-GAI1-92-KRas/N12-KRas/13C(Q61L)-GID1-mVenus-CAAX

Plasmid

#232262

Purpose

Express split-KRas fragments that are fused with CIDs and FPs

Depositor

Cliff Stains

Article

He et al ACS Chem Biol. 2025 Aug 26. doi: 10.1021

Inserts

mCerulean-GAI1-92-KRas/N12 (KRAS Human)
KRas/13C(Q61L)-GID1-mVenus-CAAX (KRAS Human)

Tags

mCerulean and mVenus

Expression

Mammalian

Mutation

KRas-Q61L

Promoter

CMV

Available Since

Sept. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRES-mCerulean-GAI1-92-KRas/N12-KRas/13C(S17N)-GID1-mVenus-CAAX

Plasmid

#232263

Purpose

Express split-KRas fragments that are fused with CIDs and FPs

Depositor

Cliff Stains

Article

He et al ACS Chem Biol. 2025 Aug 26. doi: 10.1021

Inserts

mCerulean-GAI1-92-KRas/N12 (KRAS Human)
KRas/13C(S17N)-GID1-mVenus-CAAX (KRAS Human)

Tags

mCerulean and mVenus

Expression

Mammalian

Mutation

KRas-S17N

Promoter

CMV

Available Since

Sept. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRES-mCerulean-FRB-KRas/N12-KRas/13C(Q61L)-FKBP-mVenus-CAAX

Plasmid

#214284

Purpose

Express split-KRas fragments that are fused with CIDs and FPs

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Inserts

Split-KRas fragments fused with CIDs and FPs (KRAS Human)
Split-KRas fragments fused with CIDs and FPs (KRAS Human)

Tags

mCerulean (on insert 1) and mVenus (on insert 2)

Expression

Mammalian

Mutation

KRas-Q61L

Promoter

CMV

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRES-mCerulean-FRB-KRas/N12-KRas/13C(S17N)-FKBP-mVenus-CAAX

Plasmid

#214285

Purpose

Express split-KRas fragments that are fused with CIDs and FPs

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Inserts

Split-KRas fragments fused with CIDs and FPs (KRAS Human)
Split-KRas fragments fused with CIDs and FPs (KRAS Human)

Tags

mCerulean (N terminal on insert 1) and mVenus (on…

Expression

Mammalian

Mutation

KRas-S17N

Promoter

CMV

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRES-mCerulean-GID1-KRas/N12-KRas/13C(Q61L)-GAI1-92-mVenus-CAAX

Plasmid

#214286

Purpose

Express split-KRas fragments that are fused with CIDs and FPs

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Inserts

Split-KRas fragments fused with CIDs and FPs (KRAS Human)
Split-KRas fragments fused with CIDs and FPs (KRAS Human)

Tags

mCerulean (N terminal on insert 1) and mVenus (on…

Expression

Mammalian

Mutation

KRas-Q61L

Promoter

CMV

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge