We narrowed to 9,386 results for: App

1. pGMEH-T38M-750-luxAB-DAS+4

   Plasmid

   #49520

   Purpose

   Hygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); replicates episomally

   Depositor

   Dirk Schnappinger

   Article

   Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

   Insert

   T38M-P750-luxAB-DAS+4
   

   Tags

   DAS+4 (AANDENYSENYADAS)

   Expression

   Bacterial

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. -227 hTF (mutant NF-kB)

   Plasmid

   #15443

   Depositor

   Nigel Mackman

   Article

   Oeth et al Arterioscler Thromb Vasc Biol. 1997 Feb

   Insert

   Tissue factor promoter (F3 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   NF-kappaB binding domain mutated

   Available Since

   July 27, 2007

   Availability

   Academic Institutions and Nonprofits only

3. 8xCTS1_WT-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_WT-hutRNApro_SV40polyA

   Plasmid

   #124546

   Purpose

   Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

   Depositor

   Tudor Fulga

   Article

   Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

   Insert

   WT-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_WT-hutRNApro
   

   Expression

   Mammalian

   Mutation

   WT, WT

   Promoter

   Minimal ADE

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-eDIO-ChR2-EYFP

   Plasmid

   #104387

   Purpose

   PiggyBac plasmid encoding channelrhodopsin 2 fused to EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.

   Depositor

   Volker Busskamp

   Article

   Klapper et al Sci Rep. 2017 Oct 31;7(1):14450. doi: 10

   Insert

   Channelrhodopsin 2
   

   Use

   Cre/Lox; Piggybac

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E134R

   Available Since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-myc NES- mCE(K294A)

   Plasmid

   #82464

   Purpose

   Expresses myc tag and catalytic inactive form of mRNA capping enzyme with N terminal Nuclear export signal (NES)

   Depositor

   Daniel Schoenberg

   Article

   Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

   Insert

   NES mRNA capping enzyme (Rngtt HIV, Mouse)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   K294A

   Promoter

   CMV

   Available Since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK597

   Plasmid

   #71121

   Purpose

   Produces E coli formyl-CoA:oxalate CoA-transferase (H6YfdW) in E. coli

   Depositor

   T. J. Kappock

   Article

   Mullins et al Protein Sci. 2012 May;21(5):686-96. doi:

   Insert

   formyl-CoA:oxalate CoA-transferase
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pet 3d beta tentacle delete CP

   Plasmid

   #13300

   Depositor

   John Cooper

   Article

   Wear et al Curr Biol. 2003 Sep 2. 13(17):1531-7.

   Insert

   capping protein alpha 1 beta 1 (deletion 28 amino acids ) (CAPZB Chicken)
   

   Expression

   Bacterial

   Mutation

   CP alpha subunit is intact with a mutated beta C-…

   Available Since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

8. CP3

   Plasmid

   #223335

   Purpose

   Mapping of cytosol-facing chloroplast outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

   Depositor

   Xiaohong Zhu

   Article

   Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

   Insert

   Toc64-miniTurboID-EGFP-3HA
   

   Tags

   miniTurboID-EGFP-3HA

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. CP2

   Plasmid

   #223334

   Purpose

   Mapping of cytosol-facing chloroplast outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

   Depositor

   Xiaohong Zhu

   Article

   Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

   Insert

   Toc64-EGFP-TurboID-3HA
   

   Tags

   EGFP-TurboID-3HA

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. CP1

    Plasmid

    #223333

    Purpose

    Mapping of cytosol-facing chloroplast outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

    Depositor

    Xiaohong Zhu

    Article

    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

    Insert

    Toc64-TurboID-EGFP-3HA
    

    Tags

    TurboID-EGFP-3HA

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. CP4

    Plasmid

    #223336

    Purpose

    Mapping of cytosol-facing chloroplast outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

    Depositor

    Xiaohong Zhu

    Article

    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

    Insert

    Toc64-EGFP-miniTurboID-3HA
    

    Tags

    EGFP-miniTurboID-3HA

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV_EF1a_DIO_HA/FLAG_muMASS_LF

    Plasmid

    #213394

    Purpose

    Loss of function variant of uMASS_1 opioid sensor. AAV virus production for Cre dependent expression.

    Depositor

    Andre Berndt

    Article

    Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

    Insert

    uMASS_LF
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Available Since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV_hsyn_HA_Flag-uMASS_LF

    Plasmid

    #209761

    Purpose

    AAV virus production for neuonal expression of uMASS (loss-of-function) under hSyn promoter

    Depositor

    Andre Berndt

    Article

    Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

    Insert

    uMASS_LF
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hsyn-dMASS_LF_WPRE

    Plasmid

    #209766

    Purpose

    AAV virus production for neuronal expression of dMASS_LF (loss-of-function) under hSyn promoter

    Depositor

    Andre Berndt

    Article

    Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

    Insert

    dMASS_LF
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV_hsyn_HA-Flag-uMASS_1_WPRE

    Plasmid

    #209760

    Purpose

    AAV virus production for neuronal expression of uMASS_1 under hSyn promoter

    Depositor

    Andre Berndt

    Article

    Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

    Insert

    uMASS_1
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV6-AN-HA_aa 1-542 POLK

    Plasmid

    #201585

    Purpose

    Expresses the N-terminal clasp domain and catalytic domain (aa 1-542) of human DNA polymerase kappa with a HA tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    Polymerase kappa (POLK Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Expresses amino acids 1-542 of DNA polymerase kap…

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB2

    Plasmid

    #201533

    Purpose

    Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

    Depositor

    Dan Park

    Article

    Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

    Insert

    ilvA-relE_RBS3_CB2
    

    Use

    Synthetic Biology

    Mutation

    rhaR L128+1bp (frameshift), ilvA G455C

    Promoter

    PrhaBAD (rhamnose)

    Available Since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB4

    Plasmid

    #201535

    Purpose

    Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

    Depositor

    Dan Park

    Article

    Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

    Insert

    ilvA-relE_RBS3_CB4
    

    Use

    Synthetic Biology

    Mutation

    rhaS ΔM1-H5, PrhaSR Δ179bp

    Promoter

    PrhaBAD (rhamnose)

    Available Since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. p415 δCLD2α

    Plasmid

    #73874

    Purpose

    δCLD2α - His tagged (μHD deleted)

    Depositor

    Blanche Schwappach

    Article

    Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    Arcn1/ δCLD2α (ARCN1 Bovine)
    

    Tags

    His

    Expression

    Yeast

    Promoter

    Met25

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNC2

    Plasmid

    #72438

    Purpose

    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1); NCAIR synthetase (EcPurK)

    Depositor

    T. J. Kappock, JoAnne Stubbe

    Article

    Meyer et al Biochemistry. 1999 Mar 9;38(10):3012-8.

    Inserts

    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Expression

    Bacterial

    Mutation

    Pro64 to Arg; Asp65 to His; Gln206 to Arg

    Promoter

    T7 and n/a

    Available Since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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