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pcDNA3.1/NDUFS2 _2-78/myc-His

Plasmid

#127493

Purpose

Expresses myc-tagged _2-78 NDUFS2 in mammalian cells

Depositor

Dieter Wolf, Shashi Jain

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 _2-78 (NDUFS2 Human)

Use

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 _2-78

Promoter

CMV

Available since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-hPIKfyve

Plasmid

#121148

Purpose

Expresses GFP-tagged human PIKfyve in mammalian cells.

Depositor

Geert van den Bogaart

Article

Baranov et al iScience. 2019 Jan 25;11:160-177. doi: 1

Insert

1-phosphatidylinositol 3-phosphate 5-kinase isoform 2 (PIKFYVE Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 ORF3B

Plasmid

#141272

Purpose

Gateway-compatible Entry vector, with insert of ORF3B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF3B

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV-2 S_nostop

Plasmid

#152988

Purpose

Gateway-compatible Entry vector, with insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV2 TEV-ORF9B

Plasmid

#154425

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF9B

Use

Gateway-compatible entry vector, with insert of o…

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

July 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR207 SARS-CoV-2 NSP3 Cys857Ala_nostop

Plasmid

#154029

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP3

Use

Gateway-compatible entry vector, with mutagenized…

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

June 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR207 SARS-CoV-2 NSP3 Cys857Ala

Plasmid

#154027

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP3

Use

Gateway-compatible entry vector, with mutagenized…

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

June 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV-2 N_nostop

Plasmid

#152987

Purpose

Gateway-compatible Entry vector, with insert of N gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

N

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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PC4_3spc34h

Plasmid

#70700

Purpose

Expresses S. cerevisiae DASH/Dam1 complex with C-terminal his-tag on spc34

Depositor

Stephen Harrison

Article

Miranda et al Nat Struct Mol Biol. 2005 Feb;12(2):138-

Insert

DASH / Dam1 complex (DAM1, ASK1, DUO1, SPC34, SPC19, DAD1, DAD2, DAD4, HSK3, DAD3 Budding Yeast)

Use

Tags

his-tag on C-terminus of spc34

Expression

Bacterial

Mutation

Promoter

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV2 TEV-E

Plasmid

#154418

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

E (E SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector, with insert of e…

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

July 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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PC4_3dad1h

Plasmid

#70699

Purpose

Expresses S. cerevisiae DASH/Dam1 complex with C-terminal his-tag on dad1

Depositor

Stephen Harrison

Article

Miranda et al Nat Struct Mol Biol. 2005 Feb;12(2):138-

Insert

DASH / Dam1 complex (DAM1, ASK1, DUO1, SPC34, SPC19, DAD1, DAD2, DAD4, HSK3, DAD3 Budding Yeast)

Use

Tags

his-tag on C-terminus of dad1

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV2 TEV-M

Plasmid

#154419

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

M (M SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector, with insert of m…

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

July 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV2 TEV-N

Plasmid

#154424

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

N (N SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector, with insert of n…

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

Sept. 2, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221 SARS-CoV-2 S-frag5_nostop

Plasmid

#153177

Purpose

Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth, Mikko Taipale

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S-frag5

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV2 TEV-ORF3B

Plasmid

#154417

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF3B

Use

Gateway-compatible entry vector, with insert of o…

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

July 7, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221 SARS-CoV-2 S-frag3_nostop

Plasmid

#153175

Purpose

Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth, Mikko Taipale

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S-frag3

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221 SARS-CoV-2 S-frag4_nostop

Plasmid

#153176

Purpose

Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth, Mikko Taipale

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S-frag4

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221 SARS-CoV-2 S-frag1_nostop

Plasmid

#153173

Purpose

Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth, Mikko Taipale

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S-frag1

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221 SARS-CoV-2 S-frag6_nostop

Plasmid

#153184

Purpose

Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth, Mikko Taipale

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S-frag6

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

June 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221 SARS-CoV-2 S-frag2_nostop

Plasmid

#153174

Purpose

Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth, Mikko Taipale

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S-frag2

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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