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pcDNA 3.1 (+) FLAG-Ago2 S387E

Plasmid

#60653

Purpose

Expresses CMV-FLAG-Ago2 (N-term tag) S387E mutant in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Tags

CMV-FLAG-Ago2 (N-term tag)

Expression

Mammalian

Mutation

S387E mutant

Promoter

CMV

Available Since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 (+) FLAG-Ago2 S387A

Plasmid

#60652

Purpose

Expresses CMV-FLAG-Ago2 (N-term tag) S387A mutant in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Tags

CMV-FLAG-Ago2 (N-term tag)

Mutation

S387A mutant

Promoter

CMV

Available Since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST26 Ago2 S387A

Plasmid

#59905

Purpose

Expresses 6xHis-Ago2 (N-termi tag) S387A mutant in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Tags

6xHis (N-termi tag)

Expression

Mammalian

Mutation

S387A, phospho-null alanine

Promoter

CMV

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST26 Ago2 5A

Plasmid

#60651

Purpose

Expresses 6xHis-Ago2 (N-termi tag) mutated five serines to alanines in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Tags

6xHis (N-termi tag)

Expression

Mammalian

Mutation

mutated five serines to alanines

Promoter

CMV

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST26 Ago2 4A

Plasmid

#59907

Purpose

Expresses 6xHis-Ago2 (N-termi tag) mutated four serines to alanines in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Tags

6xHis (N-termi tag)

Expression

Mammalian

Mutation

mutated four serines to alanines

Promoter

CMV

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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Anti-Glutamate carboxypeptidase 2 [J591] (Recombinant Antibody)

Antibody

#203885-rAb

Purpose

Anti-Glutamate carboxypeptidase 2 (Human) recombinant mouse monoclonal antibody

Depositor

Melina Fan

Article

Liu et al Cancer Res. 1997 Sep 1;57(17):3629-34.

Recommended Applications

Western Blot

Reactivity

Human

Source Species

Mouse

Isotype

IgG2a

Trial Size

Available to purchase

Available Since

Nov. 8, 2023

Availability

Industry, Academic Institutions, and Nonprofits
AAV-CamKIIa-ChR2-EGFP

Plasmid

#114216

Purpose

Expresses channelrhodopsin-EGFP fusion in CamKIIa+ neurons

Depositor

Xue Han

Article

Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

Insert

Channelrhodopsin 2

Use

AAV and Mouse Targeting

Tags

EGFP

Expression

Mammalian

Promoter

CamKIIa 1.3 kb

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pU6T7

Plasmid

#71462

Purpose

Expresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (2 Gs at initiation)

Depositor

Willie Mark

Article

Romanienko et al PLoS One. 2016 Feb 5;11(2):e0148362. doi

Type

Empty backbone

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

human U6/phage T7

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pk335-BFP

Plasmid

#227505

Purpose

Backbone for CARGO cloning, with U6 promoter and gRNA scaffold, TagBFP, and Kanamycin resistence

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Type

Empty backbone

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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mCer-TG2-YFP

Plasmid

#85471

Purpose

TG2 biosensor that allows for quantitative assessment of TG2 conformational changes in live cells using FRET

Depositor

Jeffrey Keillor, Ray Truant

Article

Caron et al PLoS One. 2012;7(8):e44159. doi: 10.1371

Insert

hTG2 (TGM2 Human)

Tags

EYFP and mCerulean

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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hNICD3(3xFLAG)-pCDF1-MCS2-EF1-copGFP

Plasmid

#40640

Depositor

Brenda Lilly

Article

Zhao et al J Biol Chem. 2012 May 11;287(20):16111-2

Insert

NICD3 (NOTCH3 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-Caspase-1 (C285A) -CpLxIS

Plasmid

#131355

Purpose

Expresses Caspase-1 C285A with 2 copies of pLxIS motif from STING fused to its C-ternimus

Depositor

Jonathan Kagan

Article

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

Caspase-1 (C285A)-CpLxIS (Casp1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

Caspase-1 (C285A)

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-Caspase-1 (C285A) -NpLxIS

Plasmid

#131354

Purpose

Expresses Caspase-1 C285A with 2 copies of pLxIS motif from STING fused to its N-ternimus

Depositor

Jonathan Kagan

Article

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

Caspase-1 (C285A)-NpLxIS (Casp1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

Caspase-1 (C285A)

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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Venus-Bad-2A-pEGFP-C1

Plasmid

#166748

Purpose

Express Venus fused to the N-terminus of the Bcl-2 family protein, Bad with mutation to 2 positions in the BH3 domain

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

Bad-2A (BAD Human)

Tags

Venus

Expression

Mammalian

Mutation

2 hydrophobic residues in the BH3 region mutated …

Promoter

CMV

Available Since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-ChRmine-oScarlet-KV 2.1-WPRE (AAV8)

Viral Prep

#183519-AAV8

Purpose

Ready-to-use AAV8 particles produced from pAAV-CaMKIIa-ChRmine-oScarlet-KV 2.1-WPRE (#183519). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-ChRmine-oScarlet-KV 2.1-WPRE plasmid DNA. CaMKIIa-driven expression of ChRmine-oScarlet-KV 2.1. These AAV preparations are suitable purity for injection into animals.

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Promoter

CaMKII

Available Since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only
anxA9-GFP

Plasmid

#107198

Purpose

Expresses human annexin A9-GFP fusion protein for live cell imaging

Depositor

Volker Gerke, Ursula Rescher

Article

Goebeler et al FEBS Lett. 2003 Jul 10;546(2-3):359-64.

Insert

annexin A9 (ANXA9 Human)

Tags

Green Fluorescent Protein

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pTF

Plasmid

#162784

Purpose

Protein expression in CHO cells - C-terminal FLAG and 10xhis tags are coded

Depositor

Ivan Vorobiev

Article

Kolesov et al Diagnostics (Basel). 2022 Feb 3;12(2). p

Type

Empty backbone

Tags

10xHis and FLAG

Expression

Mammalian

Promoter

CHO Elongation factor 1A

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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Syntaxin4-myc-myc-His

Plasmid

#12377

Depositor

Thomas Weimbs

Article

Low et al Mol Biol Cell. 2006 Feb . 17(2):977-89.

Insert

Syntaxin4 (STX4 Human)

Tags

2xMyc-His

Expression

Mammalian

Mutation

Cloned in modified PCDNA4 vector (double myc)

Promoter

CMV⁄TO

Available Since

Sept. 28, 2006

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag SPHK2

Plasmid

#20571

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

SPHK2 (SPHK2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pEY40

Plasmid

#191044

Purpose

ceh-2 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

ceh-2 (ceh-2 Nematode)

Expression

Worm

Mutation

None

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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