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pLEW100v5-PUR-VSG-228.200bp

Plasmid

#245113

Purpose

Inserts 200 bp of VSG-228 coding sequence in T. brucei rDNA spacer centered at AnTat1.1 position 694

Depositor

Monica Mugnier

Publication

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228:200

Use

Other

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-VSG-228.100bp

Plasmid

#245114

Purpose

Inserts 100 bp of VSG-228 coding sequence in T. brucei rDNA spacer centered at AnTat1.1 position 694

Depositor

Monica Mugnier

Publication

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228:100

Use

Other

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-VSG-228.100bp_offset

Plasmid

#245115

Purpose

Inserts 100 bp of VSG-228 coding sequence in T. brucei rDNA spacer overlapping AnTat1.1 position 694, centered around common VSG-228 insertion

Depositor

Monica Mugnier

Publication

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228:100_offset

Use

Other

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pAIP4_2H7W

Plasmid

#234220

Purpose

Heterologous protein expression of chagasin in Escherichia coli

Depositor

Christopher Bahl

Publication

Cárcamo Noriega et al Protein Sci. 2026 Sep;35(9):e70746. doi:

Insert

2H7W (Tc00.1047053506801.80 Trypanosoma cruzi)

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pLEW100v5-PUR-VSG-228.noUTRS

Plasmid

#245109

Purpose

Inserts VSG-228 coding sequence without UTRs in T. brucei rDNA spacer

Depositor

Monica Mugnier

Publication

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228

Use

Other

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-177-PUR-VSG-228

Plasmid

#245107

Purpose

Inserts VSG-228 coding sequence in T. brucei minichromosomes using the 177bp repeats

Depositor

Monica Mugnier

Publication

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228

Use

Other

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF1A06

Plasmid

#70810

Purpose

Gateway entry clone

Depositor

Alexander Stark

Publication

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

zetaTry (zetaTry Fly)

Use

Gateway Cloning

Available since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pYS108

Plasmid

#255530

Purpose

Bacterial expression of W163(5HTP) variant of human γS-crystallin

Depositor

Rachel Martin

Publication

Seo et al Biophys Rep (N Y). 2026 Mar 11;6(1):1002

Insert

W163(5HTP) variant of human γS-crystallin (CRYGS Human)

Expression

Bacterial

Mutation

Tryptophan 163 changed to 5-hydroxytryptophan

Promoter

T7

Available since

June 15, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-NEO-VSG-228

Plasmid

#245117

Purpose

Inserts VSG-228 coding sequence in T. brucei rDNA spacer

Depositor

Monica Mugnier

Publication

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228

Use

Other

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19-HYG-BES1-VSG-228-telo

Plasmid

#245104

Purpose

Inserts VSG-228 in T. brucei cells at Lister427 BES1

Depositor

Monica Mugnier

Publication

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228

Use

Other

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF1D06

Plasmid

#70813

Purpose

Gateway entry clone

Depositor

Alexander Stark

Publication

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

kappaTry (kappaTry Fly)

Use

Gateway Cloning

Available since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pT.cruzi-polyubiquitin_6xHis

Plasmid

#256080

Purpose

Production of a T. cruzi antigen, which can be used in serological assays

Depositor

Rick Tarleton

Publication

Bustamante et al PLoS Negl Trop Dis. 2022 Oct 31;16(10):e

Insert

Polyubiquitin

Tags

6xHis-STag

Expression

Bacterial

Promoter

T7lac

Available since

May 19, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19-HYG-BES1-AnTat1.1-telo

Plasmid

#245103

Purpose

Inserts VSG AnTat1.1 in T. brucei cells at Lister427 BES1

Depositor

Monica Mugnier

Publication

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

AnTat1.1

Use

Other

Mutation

silent G to A mutation at position 471 within AnT…

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pT.cruzi-microtubuleAP_6xHis

Plasmid

#256081

Purpose

Production of a T. cruzi antigen, which can be used in serological assays

Depositor

Rick Tarleton

Publication

Bustamante et al PLoS Negl Trop Dis. 2022 Oct 31;16(10):e

Insert

Microtubule-associated protein

Tags

6xHis-STag

Expression

Bacterial

Promoter

T7lac

Available since

May 18, 2026

Availability

Academic Institutions and Nonprofits only

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pGG-PIP22

Plasmid

#196760

Purpose

Contains BIK1 promoter

Depositor

Marc Somssich

Publication

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

BOTRYTIS-INDUCED KINASE 1 (BIK1 Mustard Weed)

Use

Greengate (gg) entry vector

Mutation

1878 C->G

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF3D04

Plasmid

#70967

Purpose

Gateway entry clone

Depositor

Alexander Stark

Publication

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

etaTry (etaTry Fly)

Use

Gateway Cloning

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
pT.cruzi- p25a-G10_6xHis

Plasmid

#256088

Purpose

Production of a T. cruzi antigen, which can be used in serological assays

Depositor

Rick Tarleton

Publication

Bustamante et al PLoS Negl Trop Dis. 2022 Oct 31;16(10):e

Insert

p25-alpha, putative

Tags

6xHis-T7Tag-XpressTag

Expression

Bacterial

Promoter

T7

Available since

May 28, 2026

Availability

Academic Institutions and Nonprofits only

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pT.cruzi-60s-acidic_6xHis

Plasmid

#256073

Purpose

Production of a T. cruzi antigen, which can be used in serological assays

Depositor

Rick Tarleton

Publication

Bustamante et al PLoS Negl Trop Dis. 2022 Oct 31;16(10):e

Insert

60S acidic ribosomal protein

Tags

6xHis-STag

Expression

Bacterial

Promoter

T7lac

Available since

May 19, 2026

Availability

Academic Institutions and Nonprofits only

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pT.cruzi-beta-tubulin_6xHis

Plasmid

#256086

Purpose

Production of a T. cruzi antigen, which can be used in serological assays

Depositor

Rick Tarleton

Publication

Bustamante et al PLoS Negl Trop Dis. 2022 Oct 31;16(10):e

Insert

Beta tubulin, putative

Tags

TrxA-6xHis-STag

Expression

Bacterial

Promoter

T7lac

Available since

May 18, 2026

Availability

Academic Institutions and Nonprofits only

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pT.cruzi- 60sP0-Kn107_6xHis

Plasmid

#256087

Purpose

Production of a T. cruzi antigen, which can be used in serological assays

Depositor

Rick Tarleton

Publication

Bustamante et al PLoS Negl Trop Dis. 2022 Oct 31;16(10):e

Insert

60S acidic ribosomal protein P0, putative

Tags

6xHis-T7Tag-XpressTag

Expression

Bacterial

Promoter

T7

Available since

May 28, 2026

Availability

Academic Institutions and Nonprofits only

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