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BCTV-VS domesticated-pUPD2

Plasmid

#240748

Purpose

Domesticated BCTV virion sense ORFs with A1-B1 Goldenbraid grammar

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

BCTV VS

Use

Synthetic Biology

Mutation

2nt substitution in V3

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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BCTVΔREP-pUPD2

Plasmid

#240715

Purpose

BCTV complementary sense ORFs with mutated Rep with GoldenBraid C1 grammar

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

BCTV CS

Use

Synthetic Biology

Mutation

2 stop codons inserted in Rep

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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BCTV-VS WT-pUPD2

Plasmid

#240716

Purpose

WT BCTV virion sense ORFs prior to domestication for GoldenGate cloning with A1-B1 Goldenbraid grammar

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

BCTV VS

Use

Synthetic Biology

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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PCO4SV40_pDONR201

Plasmid

#249815

Purpose

Encodes PCO4 fused to a SV40-based NLS, Gateway compatible as Donor plasmid

Depositor

Francesco Licausi

Article

Lavilla-Puerta et al bioRxiv 2025.09.01.673502

Insert

PCO4 (AT2G42670 Mustard Weed)

Use

Gateway Cloning

Tags

SV40

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1467_pTet-ECOR31Ham_hamB_walkerB_Mut

Plasmid

#223367

Purpose

Expression of ECOR31 Hachiman mutant (HamB D431A) under the pTet promoter

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

ECOR31 Hachiman mutant (HamB D431A)

Expression

Bacterial

Mutation

HamB D431A

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1469_pTet-ECOR31Ham_hamA_E138A_K140A_Mut

Plasmid

#223368

Purpose

Expression of ECOR31 Hachiman mutant (HamA E138A, K140A) under the pTet promoter

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

ECOR31 Hachiman mutant (HamA E138A, K140A)

Expression

Bacterial

Mutation

HamA E138A, K140A

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pMK024

Plasmid

#236571

Purpose

Cloning vector for PaqCI Golden Gate assembly of ISAam1 guides into ISAam1 plant expression vector

Depositor

Steven Jacobsen

Article

Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

Type

Empty backbone

Expression

Plant

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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VN1897 pB2Hws_v28_Ddcm_wkKanR_TIR51_pLWeakRBS_TIR371_cI-Asf1B+rpoA-2GG

Plasmid

#235119

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B. In fusion cloning to rpoA using BsaI based golden gate.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Type

Empty backbone

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P

Expression

Bacterial

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pET32a-Nb19-P4S

Plasmid

#236500

Purpose

Expresses anti-Apolipoprotein A1 nanobody Nb19 as a fusion protein with coiled coil P4S in bacteria.

Depositor

Alexander Green

Article

Kang et al ACS Synth Biol. 2025 Sep 3. doi: 10.1021

Insert

Nb19-P4S

Expression

Bacterial

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pET32a-301-P3S

Plasmid

#236498

Purpose

Expresses anti-RiVax nanobody 301 as a fusion protein with coiled coil P3S in bacteria.

Depositor

Alexander Green

Article

Kang et al ACS Synth Biol. 2025 Sep 3. doi: 10.1021

Insert

301-P3S

Expression

Bacterial

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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