We narrowed to 12,131 results for: LEA

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2Flag-pDEST-C

Plasmid

#118372

Purpose

Gateway compatible mammalian expression vector with C-terminal 2Flag to tag proteins of interest.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

2Flag

Expression

Mammalian

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pDisplay-iSeroSnFR

Plasmid

#128484

Purpose

Fluorescent reporter for serotonin (mammalian expression, membrane localization tag)

Depositor

Lin Tian

Article

Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

Insert

iSeroSnFR

Tags

Ig-kappa leader, Myc, PDGFR + enhanced ER export,…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pET-53-RtcB

Plasmid

#51282

Purpose

E. coli expression vector for E. coli RtcB RNA ligase

Depositor

Jay Hesselberth

Article

Peach et al Nucleic Acids Res. 2015 May 22. pii: gkv

Insert

E. coli RtcB

Tags

6xHis

Expression

Bacterial

Promoter

T7 RNA Polymerase

Available Since

March 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGreenII 0000 (No LUC)

Plasmid

#44465

Depositor

Roger Hellens

Type

Empty backbone

Use

Plant transformation

Available Since

May 6, 2013

Availability

Academic Institutions and Nonprofits only

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pExodus CMV.Artemis

Plasmid

#40211

Purpose

Mammalian expression of codon-optimized Artemis.

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2012 Oct;9(10):973-5. doi:

Insert

Artemis (DCLRE1C Human)

Expression

Bacterial and Mammalian

Promoter

CMV and T7

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-14-3-3 gamma GST

Plasmid

#13280

Depositor

Michael Yaffe

Article

Rittinger et al Mol Cell. 1999 Aug . 4(2):153-66.

Insert

14-3-3 gamma (Ywhag Rat)

Tags

GST

Expression

Bacterial

Available Since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-iSeroSnFR-Nlgn

Plasmid

#128485

Purpose

Fluorescent reporter for serotonin (dendrite localization tag)

Depositor

Lin Tian

Article

Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

Has Service

AAV9

Insert

iSeroSnFR

Use

AAV

Tags

Full-length neuroligin, Ig-kappa leader, and cpsf…

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ExRai-CKAR

Plasmid

#118409

Purpose

cpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase C activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-CKAR

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV-U6gRNA-EF(BbsI)-PGKpuro2ABFP

Plasmid

#62348

Purpose

An empty optimized gRNA expression vector

Depositor

Hiroshi Ochiai

Article

Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

Insert

none

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-I-PhoCl-HCVp

Plasmid

#87697

Purpose

Express I-PhoCl-HCVp with N-term EBFP2 in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

I-PhoCl-HCVp containing the Ki = 10.5 nM inhibitor (CP5-46A-4D5E)

Tags

EBFP

Expression

Mammalian

Promoter

CMV

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pFYF1548 EMX1

Plasmid

#47508

Purpose

human gRNA expression vector targeting EMX1

Depositor

Keith Joung

Article

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-EMX1 (EMX1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn Coff/Fon hChR2(H134R)-EYFP

Plasmid

#55648

Purpose

Cre-off/Flp-on ChR2-EYFP under the Synapsin promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

ChR2(H134R)-EYFP

Use

AAV; Cre off/flp on chr2-eyfp

Tags

EYFP

Expression

Mammalian

Promoter

hSyn

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pTetOne-mCherry-cNLS

Plasmid

#138527

Purpose

Inducible expression of mCherry with nuclear localization signal

Depositor

Christopher Grigsby, Ana Teixeira

Article

Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

mCherry

Tags

3x SV40 cNLS

Expression

Mammalian

Promoter

TRE3Gs

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-synP-HTB

Plasmid

#30195

Depositor

Edward Callaway, Liqun Luo

Article

Miyamichi et al Nature. 2011 Apr 14;472(7342):191-6. doi

Insert

histone-GFP-2A-TVA receptor-2A-rabies glycoprotein

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Available Since

April 9, 2012

Availability

Academic Institutions and Nonprofits only

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pET Flag TEV LIC cloning vector (1L)

Plasmid

#29662

Depositor

Scott Gradia

Type

Empty backbone

Tags

Flag and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pHBV-Luc

Plasmid

#71414

Purpose

To see the activity of HBV transcription using Luciferase assay

Depositor

Wang-Shick Ryu

Article

Cha et al J Gen Virol. 2009 Apr;90(Pt 4):978-86. d

Insert

HBV core promoter, enhancer I, enhancer II and luciferase

Use

Luciferase

Expression

Mammalian

Promoter

HBV

Available Since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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AAV2-miniSOG-VAMP2-T2A-mCherry

Plasmid

#50970

Purpose

AAV2 transfer vector containing the InSynC (miniSOG-VAMP2-T2A-mCherry) construct

Depositor

Roger Tsien

Article

Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

Insert

miniSOG-VAMP2-T2A-mCherry (Vamp2 Arabdopsis thaliana, Mouse)

Use

AAV

Tags

T2A-mCherry and miniSOG

Promoter

human synapsin promoter

Available Since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pSCC2

Plasmid

#39993

Depositor

Paul Modrich

Article

Cheng et al J Biol Chem. 1984 Sep 25;259(18):11571-5

Insert

EcoRI endonuclease & EcoRI methylase

Tags

none

Expression

Bacterial

Mutation

none

Promoter

n/a

Available Since

Nov. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pDY0216 PASTEv1 PE2-P2A-Bxb1

Plasmid

#179104

Purpose

PASTEv1 PE2-P2A-Bxb1

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

pCMV-PE2-P2A-Bxb1

Expression

Mammalian

Available Since

Feb. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 P-cad

Plasmid

#47502

Purpose

Expresses P-cadherin in mammalian cells

Depositor

Alice Wong

Article

Cheung et al Oncogene. 2010 Apr 22;29(16):2427-40. do

Insert

cadherin 3, type 1, P-cadherin (placental) (CDH3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only

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