We narrowed to 3,816 results for: golden gate

1. pRDA_835

   Plasmid

   #245327

   Purpose

   Cas9 CRISPRko positive control guide; targets CD59

   Depositor

   David Root, John Doench

   Insert

   sgRNA CD59 (CD59 Synthetic)
   

   Use

   Lentiviral

   Mutation

   None

   Available Since

   Oct. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLII-proUBI10_CKA3 InsPX6 K69A,K71A,K72A,K242A,H271A,K274A-FLAG_termNOS _OLE-RED

   Plasmid

   #238524

   Purpose

   Plant expression of flag-tagged A. thaliana CK2alpha3 InsPX6 K69A,K71A,K72A,K242A,H271A,K274A; with OLE1-RED marker

   Depositor

   Michael Hothorn

   Insert

   AtCK2alpha3 (AT2G23080 Mustard Weed)
   

   Tags

   FLAG

   Expression

   Plant

   Mutation

   K69A,K71A,K72A,K242A,H271A,K274A

   Available Since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTE5422

   Plasmid

   #229031

   Purpose

   sfGFP-Pepper reporter: PT7-tetO-B0035-sfGFP-pepper-T7 terminator, AmpR

   Depositor

   Tobias Erb

   Article

   Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

   Insert

   PT7-tetO_B0035-sfGFP-Pepper-T7term
   

   Expression

   Bacterial

   Promoter

   PT7-tetO

   Available Since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTE5428

   Plasmid

   #229035

   Purpose

   Expression vector for TetR: PT7-lacO-B0035-TetR-T7 terminator, AmpR

   Depositor

   Tobias Erb

   Article

   Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

   Insert

   PT7-lacO-RBS35-TetR-T7term
   

   Expression

   Bacterial

   Promoter

   PT7-lacO

   Available Since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTE5430

   Plasmid

   #229037

   Purpose

   Azurite reporter: PT7-tetO-B0035-Azurite-T7 terminator, AmpR

   Depositor

   Tobias Erb

   Article

   Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

   Insert

   PT7-tetO-B0035-Azurite-T7term
   

   Expression

   Bacterial

   Promoter

   PT7-tetO

   Available Since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBTK1052

   Plasmid

   #209542

   Purpose

   BTK Type 2 antibiotic resistance cassette for Golden Gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Lariviere et al bioRxiv 2023.09.19.558440

   Insert

   Type 2 Kanamycin resistance cassette
   

   Use

   Synthetic Biology

   Available Since

   May 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAIP4_2AWG

   Plasmid

   #234222

   Purpose

   Heterologous protein expression of peptidyl-prolyl cis-trans isomerase FKBP8 (PPIase FKBP8) in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   2AWG (FKBP8 Human)
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pAIP4_1FL0

   Plasmid

   #234216

   Purpose

   Heterologous protein expression of aminoacyl tRNA synthase complex-interacting multifunctional protein 1 in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   1FL0 (AIMP1 Human)
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pAIP4_5HFQ

   Plasmid

   #234198

   Purpose

   Heterologous protein expression of bromodomain-containing protein 2 in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   5HFQ (BRD2 Human)
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pAIP4_1TJ6

    Plasmid

    #234167

    Purpose

    Heterologous protein expression of sprouty-related, EVH1 domain-containing protein 1 (Spred-1) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    1TJ6 (spred1 Xenopus tropicalis)
    

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pAIP4_2H3L

    Plasmid

    #234160

    Purpose

    Heterologous protein expression of erbin (Densin-180-like protein) (Erbb2-interacting protein) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    2H3L (ERBIN Human)
    

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. SLEV strain Kern217 NS2B-NS3 protease fusion with non cleavable linker

    Plasmid

    #228657

    Purpose

    Bacterial expression of the fusion of the NS2B-NS3 protease from St. Louis encephalitis virus for biochemical assay

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    SLEV portion of NS2B protease fused to NS3
    

    Tags

    His-Twin Strep-Tev

    Expression

    Bacterial

    Available Since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. SPOV strain SM-6 V-1 NS2B-NS3 protease fusion with non cleavable linker

    Plasmid

    #228656

    Purpose

    Bacterial expression of the fusion of the NS2B-NS3 protease from Spondweni virus for biochemical assay

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Spondweni virus portion of NS2B protease fused to NS3
    

    Tags

    His-Twin Strep-Tev

    Expression

    Bacterial

    Available Since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. POWV strain LB NS2B-NS3 protease fusion with non cleavable linker

    Plasmid

    #228654

    Purpose

    Bacterial expression of the fusion of the NS2B-NS3 protease from Powassan virus for biochemical assay

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Powassan virus portion of NS2B protease fused to NS3
    

    Tags

    His-Twin Strep-Tev

    Expression

    Bacterial

    Mutation

    Alanine mutant to stop self-cleaving ..VEREEA*AL.…

    Available Since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. DENV-3 strain Phillippines/H87/1956 NS2B-NS3 protease fusion with non cleavable linker

    Plasmid

    #228651

    Purpose

    Bacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 3 for biochemical assays

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Dengue 3 portion of NS2B protease fused to NS3
    

    Tags

    His-Twin Strep-Tev

    Expression

    Bacterial

    Available Since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. Enterovirus A71 (EV-A71) isolate 4643-TW98 3C protease with non-cleavable C-terminal His AviTag

    Plasmid

    #228635

    Purpose

    Bacterial expression of the A71 3C protease for assays that require immobilization using biotin-streptavidin interaction

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Enterovirus A71 3C protease isoform A
    

    Tags

    His-Avi

    Expression

    Bacterial

    Available Since

    Feb. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. Enterovirus A71 (EV-A71) isolate 4643-TW98 2A protease with non-cleavable C-terminal His AviTag

    Plasmid

    #228634

    Purpose

    Bacterial expression of the A71 2A protease for assays that require immobilization using biotin-streptavidin interaction

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Enterovirus Coxsackievirus A71 2A protease
    

    Tags

    His-Avi

    Expression

    Bacterial

    Available Since

    Feb. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. Human Coxsackievirus A16 strain G10 2A protease

    Plasmid

    #228632

    Purpose

    Bacterial expression of the Coxsackievirus A16 (G-10) 2A protease this construct can be used for biochemical assay and to obtain crystals in the space group C1 2 1(PDB 8POA)

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Enterovirus Coxsackievirus A16 2A protease (HEVAgp1 Coxsackievirus 16)
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Available Since

    Feb. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pRDB_268

    Plasmid

    #231112

    Purpose

    SpG-Cas9

    Depositor

    David Root, John Doench

    Insert

    Cas9-SpG
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVP311

    Plasmid

    #222070

    Purpose

    MultiGreen 2.0 AB level 1 acceptor vector, kanamycin selection.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Greengate compatible cloning v…

    Mutation

    All unused Esp3i sites are domesticated

    Available Since

    Dec. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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