We narrowed to 1,795 results for: nif

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pSUPERpuro-BglII-Hs4E-T_AC

Plasmid

#148445

Purpose

Mammalian Expression of Hs4E-T-shRNA

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

Hs4E-T-shRNA (EIF4ENIF1 Human)

Expression

Mammalian

Available Since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-N-P13L

Plasmid

#177946

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P13L). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (P13L) (N )

Expression

Mammalian

Mutation

P13L

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-N-P80R

Plasmid

#177947

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (P80R) (N )

Expression

Mammalian

Mutation

P80R

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-N-S235F

Plasmid

#177956

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (S235F). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (S235F) (N )

Expression

Mammalian

Mutation

S235F

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-FLJ23445

Plasmid

#15429

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

FLJ23445 (Thoc7 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
Nanog-t2A-mCherry

Plasmid

#127541

Purpose

Expresses the transactivation domain of mouse Nanog and mCherry via t2A linker.

Depositor

Jennifer Mitchell

Article

Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

Insert

Nanog (Nanog Mouse)

Expression

Mammalian

Mutation

Codes for the last 110 a.a of Nanog

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAS19

Plasmid

#158788

Purpose

Ribosome-tagged GCaMP (soma localized) expression in C. elegans ASH neurons

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

psra-6::GCaMP6m::rpl-1a

Expression

Worm

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAS16

Plasmid

#158786

Purpose

Ribosome-tagged GCaMP (soma localized) expression in C. elegans AFD neurons (and others)

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

Pntc-1::GCaMP6m::rpl-1a

Expression

Worm

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pJSC090 - Bacterial expression plasmid for SpCas9 + K855A variant

Plasmid

#101213

Purpose

Bacterial expression plasmid for SpCas9 + K855A variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant K855A

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

K855A

Promoter

T7

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX-FUT5-IRES-puro

Plasmid

#250318

Purpose

Expression of human FUT5 with C-terminal FLAG tag in mammalian cells

Depositor

Jennifer Kohler

Article

Konada et al Glycobiology. 2026 Feb 26:cwag011. doi:

Insert

FUT5 (FUT5 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

synonymous mutations to Ala161 and Leu162

Promoter

CMV

Available Since

March 5, 2026

Availability

Academic Institutions and Nonprofits only

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LentiGuideFUT8-neo

Plasmid

#250316

Purpose

Encodes gRNA targeting FUT8

Depositor

Jennifer Kohler

Article

Konada et al Glycobiology. 2026 Feb 26:cwag011. doi:

Insert

FUT8 gRNA (FUT8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 5, 2026

Availability

Academic Institutions and Nonprofits only

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pLVX-FUT3-IRES-puro

Plasmid

#250317

Purpose

Expression of human FUT3 with C-terminal FLAG tag in mammalian cells

Depositor

Jennifer Kohler

Article

Konada et al Glycobiology. 2026 Feb 26:cwag011. doi:

Insert

FUT3 (FUT3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

synonymous mutations to DNA encoding Ser253 and A…

Promoter

CMV

Available Since

March 5, 2026

Availability

Academic Institutions and Nonprofits only

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pKMW306_U6-sgRNA-EMX1

Plasmid

#244824

Purpose

Mammalian expression of SpyCas9 single guide RNA targeting EMX1

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpyCas9 single guide RNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pKMW124_dCas9

Plasmid

#244821

Purpose

Mammalian expression of catalytically inactive Cas9 (dCas9)

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

dCas9 (WT)

Use

CRISPR

Tags

3xFLAG-SV40 NLS and Nucleoplasmin NLS

Expression

Mammalian

Mutation

D10A/H840A

Promoter

CAG, U6

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pHS26_10xHis_MBP_TEV_VRER-Cas9

Plasmid

#244829

Purpose

Bacterial expression of VRER Cas9 for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

VRER Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

D1135V/G1218R/R1335E/T1337R

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHS16_10xHis_MBP_TEV_xPBA-Cas9

Plasmid

#244830

Purpose

Bacterial expression of xPBA Cas9 for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

xPBA Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHS23_10xHis_MBP_TEV_SpG-Cas9

Plasmid

#244826

Purpose

Bacterial expression of SpG Cas9 for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpG Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHS25_10xHis_MBP_TEV_EQR-Cas9

Plasmid

#244828

Purpose

Bacterial expression of EQR Cas9 for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

EQR Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

D1135E/R1335Q/T1337R

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(66-140) E83A

Plasmid

#240583

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 66-140 only, E83A

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(66-140) K80A

Plasmid

#240584

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 66-140 only, K80A

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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