We narrowed to 1,670 results for: nif

1. pJSC119 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant

   Plasmid

   #101207

   Purpose

   Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant N497A/R661A/Q695A
   

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   N497A, R661A and Q695A

   Promoter

   T7

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJSC052 - Bacterial expression plasmid for SpCas9, REC3 FRET variant

   Plasmid

   #101201

   Purpose

   Bacterial expression plasmid for SpCas9, REC3 FRET variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/S701C/S960C
   

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, S701C and S960C

   Promoter

   T7

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJSC077 - Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant

   Plasmid

   #101214

   Purpose

   Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/S355C/S867C/K855A
   

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, S355C, S867C and K855A

   Promoter

   T7

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJSC033 - Bacterial expression plasmid for SpCas9, REC2 FRET variant

   Plasmid

   #101200

   Purpose

   Bacterial expression plasmid for SpCas9, REC2 FRET variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/E60C/D273C
   

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, E60C and D273C

   Promoter

   T7

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. BPK4387 - human expression plasmid for SpCas9 Cluster 5

   Plasmid

   #101191

   Purpose

   Human expression plasmid for SpCas9 Cluster 5 variant: CMV-T7-hSpCas9-Cluster5(K918A, V922A, R925A)-NLS(SV40)-3xFLAG

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster5(K918A/V922A/R925A)
   

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   K918A/V922A/R925A

   Promoter

   CMV

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. MMW3645 - human expression plasmid for SpCas9 Cluster 3

   Plasmid

   #101187

   Purpose

   Human expression plasmid for SpCas9 Cluster 3 variant: CMV-T7-hSpCas9-Cluster3(T657A, G658A, W659A, R661A)-NLS(SV40)-3xFLAG

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster3(T657A/G658A/W659A/R661A)
   

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   T657A/G658A/W659A/R661A

   Promoter

   CMV

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. MMW3770 - human expression plasmid for SpCas9 Cluster 4

   Plasmid

   #101189

   Purpose

   Human expression plasmid for SpCas9 Cluster 4 variant: CMV-T7-hSpCas9-Cluster4(F491A, M495A, T496A, N497A)-NLS(SV40)-3xFLAG

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster4(F491A/M495A/T496A/N497A)
   

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   F491A/M495A/T496A/N497A

   Promoter

   CMV

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. MMW2993 - human expression plasmid for SpCas9 Cluster 2

   Plasmid

   #101185

   Purpose

   Human expression plasmid for SpCas9 Cluster 2 variant: CMV-T7-hSpCas9-Cluster2(G582A, V583A, E584A, D585A, N588A)-NLS(SV40)-3xFLAG

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster2(G582A/V583A/E584A/D585A/N588A)
   

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   G582A/V583A/E584A/D585A/N588A

   Promoter

   CMV

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDF-5xT7-TtCsm

   Plasmid

   #128572

   Purpose

   For expression of T. thermophilus Csm in E. coli (Csm1-5)

   Depositor

   Jennifer Doudna

   Article

   Liu et al Nat Commun. 2019 Jul 5;10(1):3001. doi:

   Inserts

   Csm1
   Csm2, Csm3, Csm4, Csm5
   

   Tags

   HRV 3C protease cleavage site and 10xHis tag on C…

   Expression

   Bacterial

   Mutation

   Codon optimized for expression in E. coli and Cod…

   Promoter

   T7 promoter, lac operator

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiSIV hSyn HA-NLS-SpCas9-NLS WPRE

    Plasmid

    #236242

    Purpose

    Lentiviral expression of SpCas9

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    SpCas9
    

    Use

    Lentiviral

    Promoter

    human Synapsin 1

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. CoV2-Spike-EF1a-D614G-N501Y

    Plasmid

    #177939

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Spike proteins (D614G, N501Y). This version gives lower expression of Spike and is more convenient for generating VLPs.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Spike protein (D614G, N501Y) (S )
    

    Expression

    Mammalian

    Mutation

    D614G, N501Y

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMV-FOXA1-T2A-eGFP

    Plasmid

    #182337

    Purpose

    Expresses human FOXA1 and eGFP via T2A linker under control of a CMV promoter.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Insert

    Forkhead Box A1 (FOXA1 Human)
    

    Tags

    T2A-eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAT100

    Plasmid

    #180512

    Purpose

    Plasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, sgRNAv2 scaffold, and restriction sites to clone in new spacers

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Inserts

    CasX sgRNAv2
    PlmCasX with DpbCasX R1 loop
    

    Use

    CRISPR

    Tags

    2x FLAG and SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAT527

    Plasmid

    #180514

    Purpose

    Plasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, mNeonGreen, sgRNAv2 scaffold, and restriction sites to clone in new spacers

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Inserts

    CasX sgRNAv2
    PlmCasX with DpbCasX R1 loop-2A-mNeonGreen
    

    Use

    CRISPR

    Tags

    2x FLAG and SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIRES2-DsRed-MST-Kv2.1

    Plasmid

    #131707

    Purpose

    Expresses Kv2.1in mammalian cells

    Depositor

    Jennifer Kearney

    Article

    Jorge et al Proc Natl Acad Sci U S A. 2011 Mar 29;10

    Insert

    KCNB1 (KCNB1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJSC270 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant

    Plasmid

    #101217

    Purpose

    Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant K848A/K1003A/R1060A/N497A/R661A/Q695A/Q926A
    

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    K848A, K1003A, R1060A, N497A, R661A, Q695A and Q9…

    Promoter

    T7

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pATZ-BFP-HA-GFP11

    Plasmid

    #177721

    Purpose

    ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to blue fluorescence protein (BFP) tag protein, an HA tag and the eleventh β-strand of GFP

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    alpha-1-antitrypsin (SERPINA1 Human)
    

    Tags

    GFP11, HA, and TagBFP

    Expression

    Bacterial and Mammalian

    Mutation

    E342K (ATZ mutant)

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. SBC009876

    Plasmid

    #226283

    Purpose

    Expresses PhCFAT and ObEGS1 from trc promoter. Biosynthesis of phenylpropenes from (hydroxy)cinnamyl alcohols.

    Depositor

    Nigel Scrutton

    Article

    Robinson et al Metab Eng. 2020 Jul;60:168-182. doi: 10.

    Inserts

    Coniferyl alcohol acyltransferase
    Eugenol synthase 1
    

    Expression

    Bacterial

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJSC129 - Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant

    Plasmid

    #101211

    Purpose

    Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/E60C/D273C/N497A/R661A/Q695A/Q926A
    

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, E60C, D273C, N497A, R661A, Q695A and…

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJSC057 - Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant

    Plasmid

    #101204

    Purpose

    Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/E60C/D273C/M1–N497,GGS,V713–D1368
    

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, E60C, D273C, M1-N497, GGS, V713-D1368

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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