Addgene

We narrowed to 652 results for: weissman

Showing: 281 - 320 of 652 results

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pFA6a-mRFP1-kanMX6

Plasmid

#133551

Purpose

Yeast fluorescent (RFP) vector with PTEF promoter.

Depositor

Erin O'Shea, Won-Ki Huh

Article

Huh et al Nature. 2003 Oct 16;425(6959):686-91. do

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-mCherry-P2A-strepII-PPPCB

Plasmid

#67923

Purpose

Expresses mCherry and strepII-tag-PP2aBeta in mammalian cells

Depositor

Ron Vale

Article

Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

Insert

mCherry-P2A-StrepII-PP2aBeta (Ppp2cb Rat)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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CMV-Brainbow-2.1 R

Plasmid

#18723

Depositor

Joshua Sanes

Article

Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

Insert

hrGFPII-NLS ; EYFP ; tdimer2 ; M-mCerulean

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-scFv-GCN4-sfGFP-GB1-dWPRE

Plasmid

#60907

Purpose

The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP for imaging

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

scFv-GCN4

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-K560-E236A-24xGCN4_v1-IRES-Puro

Plasmid

#60909

Purpose

A minimal K560 kinesin rigor motor tagged with the SunTag for single molecule imaging

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

K560-E236A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pHRdSV40-K560-24xGCN4_v4

Plasmid

#72229

Purpose

A fusion of the Kinesin-1 motor domain (amino acids 1-560) to the v4 SunTag peptide array

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

K560 kinesin

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-Brainbow-1.1 M

Plasmid

#18722

Depositor

Joshua Sanes

Article

Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

Insert

Kusabira ; M-mCherry ; M-mEYFP ; M-mCerulean

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-Brainbow-1.0 L

Plasmid

#18721

Depositor

Joshua Sanes

Article

Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

Insert

dTomato ; mCerulean ; EYFP

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pLV-sgCDKN1B#2 BFP

Plasmid

#60905

Purpose

A lentiviral plasmid that encodes both a sgRNA targeting CDKN1B and BFP

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

BFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBB-XIAPmut

Plasmid

#11559

Depositor

Jon Ashwell

Article

Yang et al Science 2000 May 5;288(5467):874-7.

Insert

XIAPmut (XIAP Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

changed his 467 to Alanine

Promoter

Available since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
Thy1-Brainbow-2.1 R

Plasmid

#18727

Depositor

Joshua Sanes

Article

Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

Insert

hrGFPII-NLS ; EYFP ; tdimer2 ; M-mCerulean

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4TO-24xGCN4_v1-P2A-sfGFP

Plasmid

#61057

Purpose

sfGFP and the 24xGCN4_v1 peptide array are expressed from the same transcript, separated by a P2A site

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

24xGCN4_v1 and sfGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 17, 2014

Availability

Academic Institutions and Nonprofits only
CMV-Brainbow-1.0 H

Plasmid

#18720

Depositor

Joshua Sanes

Article

Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

Insert

tdimer2 ; M-mEYFP ; M-mCerulean

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
Thy1-Brainbow-1.1 M

Plasmid

#18726

Depositor

Joshua Sanes

Article

Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

Insert

Kusabira ; M-mCherry ; M-mEYFP ; M-mCerulean

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
Thy1-Brainbow-1.0 L

Plasmid

#18725

Depositor

Joshua Sanes

Article

Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

Insert

dTomato ; EYFP ; mCerulean

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-XIAP1-351

Plasmid

#8342

Depositor

Jon Ashwell

Article

Yang et al Science 2000 May 5;288(5467):874-7.

Insert

XIAP1-351 (XIAP Human)

Use

Tags

GST

Expression

Bacterial

Mutation

deleted amino acids 352-496

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
Thy1-Brainbow-1.0 H

Plasmid

#18724

Depositor

Joshua Sanes

Article

Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

Insert

tdimer2 ; M-mEYFP ; M-mCerulean

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-mIFP-NLS

Plasmid

#173893

Purpose

for mIFP expression in nucleus

Depositor

Ron Vale

Article

Yan et al J Cell Biol. 2021 Feb 1;220(2). pii: 211

Insert

mIFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SSFV

Available since

Nov. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBB-XIAP1-351

Plasmid

#11560

Depositor

Jon Ashwell

Article

Yang et al Science 2000 May 5;288(5467):874-7.

Insert

XIAP1-351 (XIAP Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 352-496

Promoter

Available since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
pHES553 [prGal1-RAS2(KAN) pnh604]

Plasmid

#103846

Purpose

Yeast Expression Plasmid

Depositor

Hana El-Samad

Article

Stewart-Ornstein et al Mol Biol Cell. 2017 Jan 1;28(1):221-227.

Insert

pGal1-Ras2(KAN)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-XIAPmut

Plasmid

#8341

Depositor

Jon Ashwell

Article

Yang et al Science 2000 May 5;288(5467):874-7.

Insert

XIAPmut (XIAP Human)

Use

Tags

GST

Expression

Bacterial

Mutation

H467A

Promoter

Available since

Nov. 15, 2005

Availability

Academic Institutions and Nonprofits only
pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS

Plasmid

#60904

Purpose

The plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

scFv-GCN4

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE

Plasmid

#60906

Purpose

The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP and a NLS for imaging

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

scFv-GCN4

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGST2-Rabex5 (9-73)

Plasmid

#17630

Depositor

James Hurley

Article

Lee et al Nat Struct Mol Biol. 2006 Mar . 13(3):26

Insert

Rabex5 (9-73) (RABGEF1 )

Use

Tags

GST

Expression

Bacterial

Mutation

Ubiquitin-binding domain: residues 9-73

Promoter

Available since

March 28, 2008

Availability

Academic Institutions and Nonprofits only
pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP

Plasmid

#60903

Purpose

Expressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activation

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

Cas9 dead

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pgRNA-bacteria

Plasmid

#44251

Purpose

Expression of customizable guide RNA (gRNA) for bacterial gene knockdown

Depositor

Stanley Qi

Article

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

guide RNA (bacteria)

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119 (SpeI)

Available since

April 5, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-sfCherry(1-10)

Plasmid

#70222

Purpose

Expresses sfCherry(1-10) in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

sfCherry(1-10)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
GPR_control

Plasmid

#216222

Purpose

GFP P2A RFP control reporter

Depositor

Rebecca Voorhees

Article

Inglis et al J Cell Sci. 2023 May 15;136(10):jcs26121

Insert

GFP P2A RFP_stop

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR_dSV40-Aurora A-GFP

Plasmid

#67924

Purpose

Expresses Aurora A-GFP in mammalian cells

Depositor

Ron Vale

Article

Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

Insert

GFP-Aurora A (AURKA Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pJL059

Plasmid

#177210

Purpose

Bicistronic vector with GFP reporter

Depositor

Brittany Adamson

Article

Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
inert_EJC

Plasmid

#216224

Purpose

GFP P2A RFP reporter with inert EJC immediately following stop codon

Depositor

Rebecca Voorhees

Article

Inglis et al J Cell Sci. 2023 May 15;136(10):jcs26121

Insert

GFP P2A RFP_inert_EJC

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GPR_control_lenti

Plasmid

#216223

Purpose

GFP P2A RFP control reporter in a lenti backbone

Depositor

Rebecca Voorhees

Article

Inglis et al J Cell Sci. 2023 May 15;136(10):jcs26121

Insert

GFP P2A RFP_stop

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-mCherry-Emi1_control UTRs

Plasmid

#67925

Purpose

Expresses mCherry-Emi1 with control UTRs in mammalian cells

Depositor

Ron Vale

Article

Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

Insert

mCherry-Emi1 (FBXO5 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pHES262 [prADH1-Msn2-mCherry, pnh604]

Plasmid

#103844

Purpose

Yeast Expression Plasmid

Depositor

Hana El-Samad

Article

Stewart-Ornstein et al Mol Biol Cell. 2017 Jan 1;28(1):221-227.

Insert

pAdh1-Msn2-mCherry

Use

Tags

mCherry

Expression

Yeast

Mutation

Promoter

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJR152

Plasmid

#196280

Purpose

Cassette used in dual sgRNA library generation

Depositor

Rebecca Voorhees

Article

Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

Insert

hU6-sgRNA-CR3

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-mCherry-Emi1_ARHGAP5 UTRs

Plasmid

#67927

Purpose

Expresses mCherry-Emi1 with ARHGAP5 UTRs in mammalian cells

Depositor

Ron Vale

Article

Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

Insert

mCherry-Emi1 (FBXO5 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGST2-Ub CPS (8-16)

Plasmid

#17631

Depositor

James Hurley

Article

Lee et al Nat Struct Mol Biol. 2006 Mar . 13(3):26

Insert

Ub CPS (8-16) (RPL40A Budding Yeast)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

March 28, 2008

Availability

Academic Institutions and Nonprofits only
pHR-SFFV-GFP1-10

Plasmid

#80409

Purpose

Expresses GFP1-10 in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

GFP1-10

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-GFP(1-10)

Plasmid

#70219

Purpose

Expresses GFP(1-10) in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Has Service

Cloning Grade DNA

Insert

GFP(1-10)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Ubi:Zebrabow

Plasmid

#118219

Purpose

For ubiquitous expression of Brainbow in zebrafish

Depositor

Albert Pan, Alex Schier

Article

Pan et al Development. 2013 Jul;140(13):2835-46. d

Insert

Brainbow 1.0 L

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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