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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,129 results for: 034

Showing: 141 - 160 of 16129 results

  2. pCMV-T7-ABE8e-SpCas9(MRKCRS)-P2A-EGFP (BKS1034)

    Plasmid
    #223117
    Purpose
    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MRKCRS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA8e-SpCas9(MRKCRS)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-P2A-EGFP and BPNLS-TadA8e
    Expression
    Mammalian
    Mutation
    TadA8e mutations; nSpCas9(D10A/MRKCRS)=D1135M, S1…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223117-plasmid-map-sequence-id-447641

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  5. AiP11992 - pAAV-3xSP10ins-AiE0340m-minRho-SYFP2-WPRE3-BGHpA (Alias: CN1992)

    Plasmid
    #230357
    Purpose
    AiE0340m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells
    Depositor
    Jonathan Ting, Allen Institute for Brain Science
    Article
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    SYFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    minRho
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    230357-plasmid-map-sequence-id-461900

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  6. AiP14539 - pAAV-AiE0346m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4539)

    Plasmid
    #230339
    Purpose
    AiE0346m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells
    Depositor
    Allen Institute for Brain Science, Boaz Levi
    Article
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    SYFP2-P2A-3XFLAG-10aa-H2B
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    minBG
    Available Since
    May 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    230339-plasmid-map-sequence-id-473573

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  7. AiP14537 - pAAV-AiE0344m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4537)

    Plasmid
    #230338
    Purpose
    AiE0344m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells
    Depositor
    Allen Institute for Brain Science, Boaz Levi
    Article
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    SYFP2-P2A-3XFLAG-10aa-H2B
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    minBG
    Available Since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    230338-plasmid-map-sequence-id-470599

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  8. AiP14538 - pAAV-AiE0345m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4538)

    Plasmid
    #230165
    Purpose
    AiE0345m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells
    Depositor
    Allen Institute for Brain Science, Boaz Levi
    Article
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    SYFP2-P2A-3XFLAG-10aa-H2B
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    minBG
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    230165-plasmid-map-sequence-id-461890

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  9. pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (AAV8)

    Viral Prep
    #154867-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (#154867). In addition to the viral particles, you will also receive purified pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA plasmid DNA. Syn-driven, Flp-dependent hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ulrik Gether
    Article
    Runegaard et al eNeuro. 2018 Jun 18;5(3). pii: eN-NWR-03
    Promoter
    Human Synapsin-1
    Tags
    mCherry (Flp-dependent)
    Available Since
    Aug. 19, 2022
    Availability
    Academic Institutions and Nonprofits only

  10. pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (AAV Retrograde)

    Viral Prep
    #154867-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (#154867). In addition to the viral particles, you will also receive purified pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA plasmid DNA. Syn-driven, Flp-dependent hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ulrik Gether
    Article
    Runegaard et al eNeuro. 2018 Jun 18;5(3). pii: eN-NWR-03
    Promoter
    Human Synapsin-1
    Tags
    mCherry (Flp-dependent)
    Available Since
    Oct. 8, 2020
    Availability
    Academic Institutions and Nonprofits only

  11. Human ubiquitination-related proteins CRISPR KO library

    Pooled Library
    #174592
    Purpose
    CRISPR Cas9 KO library targets 660 ubiquitination-related proteins, including all major E1, E2, E3 and deubiquitinating enzymes, as well as other ubiquitination-related proteins.
    Depositor
    Juan Bonifacino
    Article
    Jia et al Elife. 2019 Nov 6;8. pii: 50034. doi: 10
    Expression
    Mammalian
    Species
    Homo sapiens
    Use
    CRISPR and Lentiviral
    Available Since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 141 - 160 of 16129 results