We narrowed to 864 results for: Anti-CRISPR

Showing: 141 - 160 of 864 results

Results per page:

Sort results by:

AAV CMV-driven AcrIIA4-2xmiR-1 target sites

Plasmid

#120299

Purpose

AAV vector for expression of AcrIIA4 with two miR-1 binding sites

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

AcrIIA4-2xmiR-1 binding sites

Use

AAV and CRISPR

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV CMV-driven AcrIIC1-2xmiR-122 target sites

Plasmid

#120302

Purpose

AAV vector for expression of AcrIIC1 with two miR-122 binding sites

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

AcrIIC1-2xmiR-122 binding sites

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV CMV-driven AcrIIC3-2xmiR-122 target sites

Plasmid

#120303

Purpose

AAV vector for expression of AcrIIC3 with two miR-122 binding sites

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

AcrIIC3-2xmiR-122 binding sites

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only
pAAV-CMV-CASANOVA

Plasmid

#113036

Purpose

AAV vector for CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV Intein-C-SpyCas9, CMV-AcrIIA4-scaffold (2xBsmBI sites)

Plasmid

#120294

Purpose

AAV Vector for expression of C-terminal SpyCas9 fragemnt with split-intein and a CMV-driven AcrIIA4 (no miR binding sites)

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

C-terminal fragment of SpyCas9

Use

AAV and CRISPR

Tags

split-intein

Expression

Mammalian

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AcrVA1_AsLOV2-S103ΔELS

Plasmid

#246049

Purpose

Expression of AcrVA1-AsLOV2 hybrid in mammalian cells; insertion before S103 with deletion of surrounding amino acids ELS; enables optogenetic control of MbCas12a

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrVA1_AsLOV2-S103ΔELS

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Mutation

ΔELS

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AcrVA1_cpGR2(GGS5)-S103ΔELS

Plasmid

#246050

Purpose

Expression of AcrVA1-cpGR2 hybrid in mammalian cells; insertion before S103 with deletion of surrounding amino acids ELS; enables chemogenetic control of MbCas12a

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrVA1_cpGR2-S103ΔELS

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Mutation

ΔELS

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
H1_sgRNA(GRIN2B)_SauCas9

Plasmid

#246054

Purpose

Expression of a sgRNA targeting the GRIN2B locus and SauCas9 under the same pol-III H1 promoter

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

SauCas9 and sgRNA(GRIN2B)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV_AcrIIC3_cpGR2(GGS5)-F59

Plasmid

#246053

Purpose

Expression of AcrIIC3-cpGR2 hybrid in mammalian cells; insertion before F59; enables chemogenetic control of NmeCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIC3_cpGR2-F59

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV_AcrIIA4_cpGR2(GGS5)-ΔN64_Q65_E66

Plasmid

#246052

Purpose

Expression of AcrIIA4-cpGR2 hybrid in mammalian cells after Bubeck et al Nat Methods 2018; enables chemogenetic control of SpyCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA4_cpGR2-ΔN64/Q65/E66

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Mutation

ΔN64/Q65/E66

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV_AcrIIA5_cpGR2(GGS5)-N77

Plasmid

#246046

Purpose

Expression of AcrIIA5-cpGR2 hybrid in mammalian cells; insertion before N77; enables chemogenetic control of SauCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA5_cpGR2-N77

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV_AcrIIA5_cpGR2(GGS5)-D41

Plasmid

#246045

Purpose

Expression of AcrIIA5-cpGR2 hybrid in mammalian cells; insertion before D41; enables chemogenetic control of SauCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA5_cpGR2-D41

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV_AcrIIA5_AsLOV2-N77

Plasmid

#246044

Purpose

Expression of AcrIIA5-AsLOV2 hybrid in mammalian cells; insertion before N77; enables optogenetic control of SauCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA5_AsLOV2-N77

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV_AcrIIA5_AsLOV2-D41

Plasmid

#246043

Purpose

Expression of AcrIIA5-AsLOV2 hybrid in mammalian cells; insertion before D41; enables optogenetic control of SauCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA5_AsLOV2-D41

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV Intein-C-SpyCas9, CMV-AcrIIA4-2xmiR-122 target sites

Plasmid

#120295

Purpose

AAV Vector for expression of C-terminal SpyCas9 fragement with split-intein and a CMV-driven AcrIIA4 with two miR-122 binding sites

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

C-terminal fragment of SpyCas9

Use

AAV and CRISPR

Tags

split-intein

Expression

Mammalian

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)

Plasmid

#171129

Purpose

LwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)

Depositor

Benjamin Kleinstiver

Article

Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

Insert

U6 promoter entry plasmid for LwaCas13a crRNA cloning

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-null (BPK1077)

Plasmid

#171128

Purpose

Plasmid encoding a pCMV-T7 with a 2 amino acid peptide prior to a stop codon.

Depositor

Benjamin Kleinstiver

Article

Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

Insert

pCMV-T7 null expression plasmid that encodes a two amino acid peptide (Met-Asp)

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

pCMV and T7

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p1192-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS

Plasmid

#129530

Purpose

Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme

Depositor

Erik Sontheimer

Article

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Inserts

codon-optimized AcrIIC3Nme
sgRNA_Rosa26

Use

AAV

Tags

FLAG/NLS

Promoter

U6 and cytomegalovirus-enhancer chicken β-actin p…

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
p1194-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIA4_FLAG_NLS

Plasmid

#129532

Purpose

Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIA4

Depositor

Erik Sontheimer

Article

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Inserts

codon-optimized AcrIIA4
sgRNA_Rosa26

Use

AAV

Tags

FLAG/NLS

Promoter

U6 and cytomegalovirus-enhancer chicken β-actin p…

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-RSV-GFP-U6-gRNA scaffold (SpyCas9)

Plasmid

#113039

Purpose

AAV vector; encodes GFP as well as a U6-driven gRNA scaffold (SpyCas9)

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

2x BbsI sites - SpCas9 scaffold, co-expressed GFP (transfection marker)

Use

AAV

Expression

Mammalian

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge