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Plasmid#50393PurposeSSR2.0 has two target sites in opposite orientation for generate only one cycle of repairing after I-SceI endonuclease expression.DepositorInsertSSR2.0
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
SeeSaw Reporter (SSR1.0)
Plasmid#50392PurposeSSR1.0 has a one I-SceI target site for generate specific double strand break after I-SceI endonuclease expression.DepositorInsertSSR1.0
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(PGKCMV)-nucTomato-hygro
Plasmid#208573PurposeLV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an PGK/CMV promoter driving nuclear d2eGFP E2A HygromycinDepositorInsertsmnucTomato
d2nucEGFP
HygroR
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterPGK/CMV and minPAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(PGK/CMV)-GtwyA
Plasmid#208574PurposeLV REPORT containing minimal promoter (GtwyA inserted into MCS) driving monomeric nuclear Tomato followed by an PKG/CMV promoter driving nuclear d2eGFP E2A HygromycinDepositorInsertsmnucTomato
HSVtk
Neomycin
d2nucEGFP
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterPGK/CMV and minPAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETcLIC-GFP
Plasmid#183514PurposeThe plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in E. coliDepositorTypeEmpty backboneUseTags10x His-tag and folding reporter GFPExpressionBacterialMutationPromoterT7 lacAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYUBcLIC-GFP
Plasmid#183509PurposeThe plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in mycobacteria.DepositorTypeEmpty backboneUseTags10x His-tag and folding reporter GFPExpressionBacterialMutationPromoterT7 lacAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(EF1a)TetR
Plasmid#172352PurposeTet Repressor reporterDepositorInsertsTetR
d2eGFP
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianMutationPromoterEF1alphaAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2_mCherry_roGFP2_Orp1
Plasmid#155043PurposeFluorescent redox reporterDepositorInsertOrp1 (HYR1 Budding Yeast)
UseLentiviralTagsroGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT (PGK) NanoLuc
Plasmid#208576PurposeEmpty NanoLuc Lentiviral reporterDepositorInsertsNanoLuciferase
d2eGFP
Empty response element
UseLentiviral, Luciferase, and Synthetic BiologyTags3x SV40 NLS and PESTExpressionMammalianMutationPromoterPGK and miniPAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(PGK)-12xHRE
Plasmid#172334Purposehypoxic responsive reporterDepositorInsertsmonomeric nuclear Tomato
d2eGFP
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianMutationPromoterPGKAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pLV-REPORT(PGK/CMV)-12xHRE
Plasmid#172335Purposehypoxic responsive reporterDepositorInsertsmonomeric nuclear Tomato
d2eGFP
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianMutationPromoterPGK/CMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT (PGK) 12xHRE-NanoLuc
Plasmid#208575Purposehypoxic response element driven NanoLuc Lentiviral reporterDepositorInsertsNanoLuciferase
d2eGFP
12xHRE
UseLentiviral, Luciferase, and Synthetic BiologyTags3x SV40 NLS and PESTExpressionMammalianMutationPromoterAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(PGK/CMV)-GRE
Plasmid#172336PurposeGal4 Response elementDepositorInsertsmonomeric nuclear Tomato
d2eGFP
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianMutationPromoterPGK/CMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2_mCherry_Grx1_roGFP2
Plasmid#155045PurposeFluorescent redox reporterDepositorInsertGlutaredoxin-1 (GLRX Human)
UseLentiviralTagsroGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT (PGK) 5xGRE-NanoLuc
Plasmid#207170PurposeGAl4 response element driven NanoLuc Lentiviral reporterDepositorInsertsNanoLuciferase
d2eGFP
5x GRE
UseLentiviral, Luciferase, and Synthetic BiologyTags3x SV40 NLS and PESTExpressionMutationPromoterGal4 response element minP and PGKAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-GFP
Plasmid#158130PurposeSingle fluorescent protein reporter (GFP) for ncAA incorporation (wild-type construct)DepositorInsertsfGFP
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAMS366-4xCDRE-GFP
Plasmid#138657Purposestable GFP reporter to monitor calcineurin activity via calcineurin-dependent response element (CDRE) in S. cerevisiaeDepositorInsert4xCDRE-CYC1(PM)-yeGFP
UseYeast reporter plasmidTagsExpressionMutationPromoterCYC1Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAMS366-4xCDRE-GFP-PEST
Plasmid#138658Purposedestabilized GFP reporter to monitor calcineurin activity via calcineurin-dependent response element (CDRE) in S. cerevisiaeDepositorInsert4xCDRE-CYC1(PM)-yEGFP3-PEST
UseYeast reporter plasmidTagsExpressionMutationPromoterCYC1Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGCGFP-puro
Plasmid#31266DepositorInsertGCGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only