We narrowed to 268 results for: H2A

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pME-zFUCCI-P2A-H2A-tdiRFP (JDW 1380)

Plasmid

#242559

Purpose

Gateway middle entry clone containing a zebrafish FUCCI reporter followed by H2A tdiRFP reporter; For visualizing cell cycle state.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCerulean-zGeminin(1-100) P2A mCherry-zCdt1(1-190)

Use

Gateway subcloning

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1394 (pME-ZIC3-P2A-H2A-mCherry)

Plasmid

#232133

Purpose

A middle entry gateway clone containing V5 tagged human ZIC3 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

ZIC3 (ZIC3 Human)

Use

Gateway cloning

Tags

V5

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1391 (pME-V5-FOXF1-P2A-H2A-mCherry)

Plasmid

#232132

Purpose

A middle entry gateway clone containing V5 tagged human FOXF1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

FOXF1 (FOXF1 Human)

Use

Gateway cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-Luciferase-P2A-H2A-mCherry (JDW 1129)

Plasmid

#229823

Purpose

A CAGGS driven luciferase reporter followed by a P2A cleavage peptide and an H2A mCherry cassette for nuclear labeling.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Luciferase-P2A-H2A-mCherry

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pB-Tet-Off_FLEX_Luc-P2A-H2A-mCherry (JDW 970)

Plasmid

#229824

Purpose

A PiggyBac vector with a cre-dependent dual luciferase / nuclear mCherry reporter. In the presence of cre, this tet-off vector will be ubiquitously expressed in the absence of dox/tet

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Luciferase-P2A-H2A-mCherry

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 945)

Plasmid

#229837

Purpose

A Piggybac compatible, tet-on, NEO selectable, vector for expressing luciferase followed by a P2A cleavage peptide and H2A fused mCherry.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Luciferase

Expression

Mammalian

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 1154)

Plasmid

#229840

Purpose

A Piggybac compatible, tet-on, dual reporter encoding luciferase followed by a P2A cleavage peptide and then an H2A fused mCherry for nuclear labeling.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Luciferase

Expression

Mammalian

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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p3E-IRES-H2A-mCherry-SV40-pA (JDW 1256)

Plasmid

#224532

Purpose

A Gateway compatible 3' entry clone containing an IRES H2A mCherry followed by a polyA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

IRES-H2A-mCherry-SV40pA

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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JDW 1475 (pLenti-BLAST-GATA4-P2A-H2A-mCherry)

Plasmid

#233549

Purpose

A CMV driven human GATA4 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

GATA4 (GATA4 Human)

Use

Lentiviral

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Flag-H2A K5-9-118-119-125-127-129R

Plasmid

#63565

Purpose

Expression of human H2A (with multiple Lysine to Arginine point mutations) tagged with Flag

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC20 Human)

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Mutation

Lysines at 5, 9, 118, 119, 125, 127, and 129 are…

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
AttB_[Kozak-mut]ACE2_IRES-mCherry-H2A-P2A-PuroR

Plasmid

#171595

Purpose

Weak human ACE2 abundance from Bxb1 landing pad

Depositor

Kenneth Matreyek

Article

Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

Inserts

ACE2 (ACE2 Human)
mCherry
H2A
Pac

Use

Synthetic Biology; Promoterless bxb1 dna recombin…

Expression

Mammalian

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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JDW 1392 (pLenti-CMV-FOXF1-P2A-H2A-mCherry)

Plasmid

#233546

Purpose

A CMV driven human FOXF1 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

FOXF1 (FOXF1 Human)

Use

Lentiviral

Tags

FLAG

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1473 (pLenti-BLAST-ZIC3-P2A-H2A-mCherry)

Plasmid

#233547

Purpose

A CMV driven human ZIC3 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

ZIC3 (ZIC3 Human)

Use

Lentiviral

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen

Plasmid

#129631

Purpose

Visualization of Akt and ERK activity

Depositor

Dorus Gadella

Article

Chavez-Abiega et al J Cell Sci. 2022 Mar 15;135(6):jcs259685

Insert

H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pET29a-YS14

Plasmid

#66890

Purpose

Polycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 and H4)

Depositor

Jung-Hyun Min

Article

Shim et al Anal Biochem. 2012 Aug 15;427(2):190-2.

Inserts

His6-Thrombin-Xenopus laevis histones H2A
Xenopus laevis histones H2B
Xenopus laevis histones H3
Xenopus laevis histones H4-Thrombin

Tags

H2A, H2B, H3, H4, His6 tag, and Thrombin

Expression

Bacterial

Promoter

T7

Available Since

Sept. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pET29a-human histone 4-in-One

Plasmid

#223237

Purpose

Polycistronic bacterial coexpression construct for human His-Sumo-H2A, H2B, H3, and Dual-StrepII-H4

Depositor

Weixing Zhao

Inserts

His6-VSVG-Histone H2A
Histone H2B
Histone H3
Histone H4-TEV-His6-Dual StrepII

Tags

Dual StrepII, His6, TEV protease site, and VSVG

Expression

Bacterial

Mutation

A103G

Promoter

T7

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pTLR-donor

Plasmid

#118999

Purpose

Repair template for the TLR reporter

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

mVenus

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-i53

Plasmid

#119002

Purpose

Mammalian expression of i53 (53BP1) inhibitor and BFP

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

i53

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Rad18

Plasmid

#119003

Purpose

Mammalian expression of human Rad18 and BFP

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

Rad18

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pTLR-donor-UAS

Plasmid

#119001

Purpose

Repair template for the TLR reporter with 10 UAS binding sites for yeast Gal4

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

mVenus

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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