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Plasmid#178334PurposeFluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZDepositorInsertpAAV CAG iGluSnFR3 v857.SGZ
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsIgK-chain, Myc epi tag, and Stargazin and PDZ dom…ExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.FLEX.iGluSnFR3.v82.GPI.codonopt
Plasmid#175184PurposeFluorescent reporter for glutamate, third generation, variant 82 in GPI backbone. iGluSnFR3.v82.GPIDepositorInsertpAAV hSyn FLEX iGluSnFR3 v82.GPI
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsGPI Anchor, IgK-chain, and Myc epi tagExpressionMammalianMutationPromoterhuman SynapsinAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.FLEX.iGluSnFR3.v857.PDGFR.codonopt
Plasmid#175180PurposeFluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857DepositorHas ServiceAAV1InsertpAAV hSyn FLEX iGluSnFR3 v857.PDGFR
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsIgK-chain, Myc epi tag, and PDGFR TM DomainExpressionMammalianMutationPromoterhuman SynapsinAvailable sinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.iGluSnFR3.v857.SGZ
Plasmid#178337PurposeFluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZDepositorInsertpAAV GFAP iGluSnFR3 v857.SGZ
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsIgK-chain, Myc epi tag, and Stargazin and PDZ dom…ExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.FLEX.iGluSnFR3.v857.SGZ.codonopt
Plasmid#175182PurposeFluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZDepositorInsertpAAV hSyn FLEX iGluSnFR3 v857.SGZ
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsIgK-chain, Myc epi tag, and Stargazin and PDZ dom…ExpressionMammalianMutationPromoterhuman SynapsinAvailable sinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-flex-iGluSnFR4f-PDGFR-WPRE
Plasmid#234448PurposeAAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.DepositorInsertiGluSnFR4f-PDGFR
UseAAV and Cre/LoxTagsIgK chain and Myc epi tag-PDGFR TM DomainExpressionMammalianMutationPromoterCAGAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-iGluSnFR4s-PDGFR-WPRE
Plasmid#234449PurposeAAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.DepositorInsertiGluSnFR4s-PDGFR
UseAAVTagsIgK chain and Myc epi tag-PDGFR TM DomainExpressionMammalianMutationPromoterCAGAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-FLP-FRT-iGluSnFR4s-NGR-WPRE
Plasmid#234453PurposeAAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.DepositorInsertiGluSnFR4s-NGR
UseAAV; Flp/frtTagsIgK chain and Myc epi tag-NGR GPI anchorExpressionMammalianMutationPromoterSynapsinAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-FLP-FRT-iGluSnFR4f-NGR-WPRE
Plasmid#234454PurposeAAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.DepositorInsertiGluSnFR4f-NGR
UseAAV; Flp/frtTagsIgK chain and Myc epi tag-NGR GPI anchorExpressionMammalianMutationPromoterSynapsinAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTS1037
Plasmid#169616PurposeTier-2 vector encoding PmPGK1-driven secreted Nluc (A1-pA::A2-pA::PmPGK1-IgkSS-Nluc-pA).DepositorInsertPPGK-driven expression of secreted Nluc
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1035
Plasmid#169614PurposeTier-2 vector encoding PmPGK1-driven secreted Nluc (PmPGK1-IgkSS-Nluc-pA::A2-pA::A3-pA).DepositorInsertPPGK-driven expression of secreted Nluc
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1032
Plasmid#169612PurposeTier-2 vector encoding PhCMV-driven secreted Nluc (A1-pA::PhCMV-IgkSS-Nluc-pA::A3-pA).DepositorInsertPCMV-driven expression of secreted Nluc
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1031
Plasmid#169611PurposeTier-2 vector encoding PhCMV-driven secreted Nluc (PhCMV-IgkSS-Nluc-pA::A2-pA::A3-pA).DepositorInsertPCMV-driven expression of secreted Nluc
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1033
Plasmid#169613PurposeTier-2 vector encoding PhCMV-driven secreted Nluc (A1-pA::A2-pA::PhCMV-IgkSS-Nluc-pA).DepositorInsertPCMV-driven expression of secreted Nluc
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1036
Plasmid#169615PurposeTier-2 vector encoding PmPGK1-driven secreted Nluc (A1-pA::PmPGK1-IgkSS-Nluc-pA::A3-pA).DepositorInsertPPGK-driven expression of secreted Nluc
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
GreenT-EC - GPI
Plasmid#193946PurposeMammalian expression vector for a low affinity calcium indicator exposed to the cell surfaceDepositorInsertGreenT-EC
UseTagsGPI-anchor and Igk_secretion - HA-FlagExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
GreenT-EC - Display - CyRFP1
Plasmid#193943PurposeMammalian expression vector for a low affinity calcium indicator exposed to the cell surfaceDepositorInsertGreenT-EC
UseTagsIgk_secretion - HA-Flag and PDGFRExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
GreenT-EC.b - Display - CyRFP1
Plasmid#193944PurposeMammalian expression vector for a low affinity calcium indicator exposed to the cell surfaceDepositorInsertGreenT-EC.b
UseTagsIgk_secretion - HA-Flag and PDGFRExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
GreenT-EC.c - Display - CyRFP1
Plasmid#193945PurposeMammalian expression vector for a low affinity calcium indicator exposed to the cell surfaceDepositorInsertGreenT-EC.c
UseTagsIgk_secretion - HA-Flag and PDGFRExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS2311-Tier1-spacer-PPGK-NLuc_TB
Plasmid#169552PurposeTier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertPPGK-NanoLuc Luciferase - mTagBFP fusion
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only