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Plasmid#183702PurposeEncode human Ig kappa chain constant region. For cloning variable region and mAb expression.DepositorInsertIGKC (IGKC )
UseTagsExpressionMammalianMutationPromoterSP6Available sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
AbVec1.1-IGKC
Plasmid#80796PurposeExpression of immunoglobulin light chains in mammalian cells, human kappa constant regionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.0-mIgkc
Plasmid#127157PurposeExpression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), kappa constant regionDepositorInsertIgkc (Igkc Mouse)
UseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pST_IGKV_ANDDA_Unigene43915 (pBS0336)
Plasmid#185181PurposeFor the mammalian expression of the Chinese giant salamander protein IGKV_ANDDA_Unigene43915. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertIGKV_ANDDA_Unigene43915
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
IgK-IFN-luc
Plasmid#14886PurposeReporter construct for NF‐κB activation. Contains two copies of immunoglobulin kappa light chain motif (5′‐GGGGACTTTCC‐3′) upstream of the a minimal promoter driving luciferase expressionDepositorInsertIgK-IFN (IFNB1 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_IgKchL_HA_HaloTag9_myc_PDGFRtmb
Plasmid#175544PurposeExpression of HaloTag9 at the outer leaflet of the plasma membrane of mammalian cellsDepositorInsertIgKchL_HA-HaloTag9-myc_PDGFRtmb
UseTagsExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_IgKchL_HA_STf_myc_PDGFRtmb
Plasmid#182009PurposeCMV overexpression of SNAP-tag fast at the outer leaflet of the plasma membrane of mammalian cell linesDepositorInsertIgKchL_HA_STf_myc_PDGFRtmb
UseTagsExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmb
Plasmid#175534PurposeExpression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cellsDepositorInsertIgKchL_HA-HaloTag7-myc_PDGFRtmb
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIgK-2.1-FLAG
Plasmid#225829PurposeMouse IgK chain specific for chicken gamma globulin (clone 2.1). Used to produce FLAG-tagged monoclonal antibodies for use as tag-specific ELISA standards.DepositorInsertMouse anti-CGG IgK chain, Flag-tagged
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIgK-2.1-StreptagII
Plasmid#225830PurposeMouse IgK chain specific for chicken gamma globulin (clone 2.1). Used to produce StreptagII-tagged monoclonal antibodies for use as tag-specific ELISA standards.DepositorInsertMouse anti-CGG IgK chain, Streptag-II-tagged
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-P3-IgKL-mNG
Plasmid#183465PurposeTo produce AAV with the P3 promoter to express IgkL-mNeonGreen (IgKL is a secretory signal peptide)DepositorInsertIgKL-mNeonGreen
UseAAVTagsIgKL secretory signalExpressionMutationPromoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
IgK-BmTyr-KDEL
Plasmid#222942PurposeER-localized BmTyrDepositorInsertIgK-BmTyr-Flag-KDEL
UseTagsFlag tagExpressionMammalianMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.iGluSnFR3.v857.IgK-NGR
Plasmid#196227PurposeFluorescent reporter for glutamate, third generation, variant 857 in NGR backbone. iGluSnFR3.v857.NGRDepositorInsertpAAV hSyn iGluSnFR3 v857.NGR
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsIgK-chain, Myc epi tag, and NGR TM DomainExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS2395-Tier1-PhCMV-Igk_mRuby2_KDEL
Plasmid#169533PurposeTier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 localized to the ER (PhCMV-Igk-mRuby2-KDEL-pA).DepositorInsertPCMV-driven ER-localized monomeric red fluorescent protein mRuby2
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-IGK-dAPEX2-KDEL
Plasmid#117182PurposePlasmid name in publication: pAAV-ER-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertIGK-dAPEX2-KDEL
UseAAVTagsExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.FLEX.iGluSnFR3.v857.IgK-NGR
Plasmid#196226PurposeFluorescent reporter for glutamate, third generation, variant 857 in NGR backbone. iGluSnFR3.v857.NGRDepositorInsertpAAV hSyn FLEX iGluSnFR3 v857.NGR
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsIgK-chain, Myc epi tag, and NGR TM DomainExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CBh-IgKL-mNG
Plasmid#183467PurposeTo produce AAV with the CBh promoter to express IgkL-mNeonGreen (IgKL is a secretory signal peptide)DepositorInsertIgKL-mNeonGreen
UseAAVTagsIgKL secretory signalExpressionMutationPromoterAvailable sinceMay 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
MP28445 – pFUSE2ss-aRD114_CLIg-mIgK
Plasmid#110556PurposepFUSE backbone with an IL2 signal sequence, expressing the variable light chain of anti-RD114 binder fused to murine Kappa constant light chain.DepositorInsertanit-RD114 light chain
UseTagsmKappaExpressionBacterial and MammalianMutationPromoterEF-1 alpha core promoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D -IgK-TurboID-HA
Plasmid#219810PurposeSecreted HA-tagged TurboID for extracellular proximity labelingDepositorInsertIgK (Igk Mouse)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMM585-Tier1-PhCMV-Igk
Plasmid#169486PurposeTier-1 vector encoding PhCMV-driven secretion signal Igk (PhCMV-Igk-pA).DepositorInsertPCMV-driven Igk secretion signal (IGK Human)
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only