Addgene

We narrowed to 357 results for: NS1

Showing: 141 - 160 of 357 results

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pFastBac (GST-SNAP-Shp2)

Plasmid

#177897

Purpose

Baculo expression of GST tag fused to SNAP tag and human Shp2

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

Shp2 (PTPN11 Human)

Use

Tags

TEV-GST-PreScission cut site-SNAP tag

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac(His10-Shp2)

Plasmid

#177899

Purpose

Baculo expression of His10 tag fused to human Shp2

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

Shp2 (PTPN11 Human)

Use

Tags

10xHis-TEV-SNAP tag

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

April 11, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-SV40-mCherry-SHP1 (SHP2-nSH2, cSH2)

Plasmid

#180823

Purpose

Expression of N-terminal mCherry tagged human SHP1 with its nSH2 and cSH2 replaced by nSH2 and cSH2 of SHP2

Depositor

Enfu Hui

Article

Xu et al Elife. 2021 Nov 4;10. pii: 74276. doi: 1

Insert

SHP1 (SHP2-nSH2, cSH2) (PTPN6, PTPN11 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SV40

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX6P2(GST-SNAP-Shp2tSH2)

Plasmid

#177907

Purpose

Baculo expression of GST tag fused to SNAPtag and human Shp2 tandem SH2 domains

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

Shp2 tandem SH2 domains (aa 6-218 only) (PTPN11 Human)

Use

Tags

GST-PreScission cut site-SNAP tag

Expression

Bacterial

Mutation

Promoter

tac

Available since

Availability

Academic Institutions and Nonprofits only

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pGEX tensin-SH2

Plasmid

#46525

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

tensin (TNS1 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1451-1588

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX SHP-2(N)-SH2

Plasmid

#46498

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHP-2(N) (PTPN11 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1-104

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX SHP-2(NC)-SH2

Plasmid

#46499

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHP-2(NC) (PTPN11 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1-222

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX SHP-2(C)-SH2

Plasmid

#46497

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHP-2(C) (PTPN11 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 101-222

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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XX01CAPN1A-c001

Plasmid

#175491

Purpose

Protein expression in bacterial cells. Entire CDS, M1-A714 (CAPN1); EF hand 1-5, H86-S268 (CAPNS1). CAPN1 can be biotinylated. Codon optimised.

Depositor

Opher Gileadi

Insert

CAPN1 / CAPNS1 (CAPN1 Human)

Use

Tags

AviTag and His6-TEV

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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XX01CAPN2A-c001

Plasmid

#175492

Purpose

Protein expression in bacterial cells. Entire CDS, M1-L700 (CAPN2); EF hand 1-5, H86-S268 (CAPNS1). CAPN2 can be biotinylated. Codon optimised.

Depositor

Opher Gileadi

Insert

CAPN2 / CAPNS1 (CAPN2 Human)

Use

Tags

AviTag and His6-TEV

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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piHGpd/p82

Plasmid

#1105

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

wild-type HSP82 regulated by the strong constitut…

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
piHGpd/G170D

Plasmid

#1113

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

HSP82 with glycine 170 changed to aspartic acid; …

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
piHGpd/T101I

Plasmid

#1112

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

HSP82 with threonine 101 changed to isoleucine; r…

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
piHGpd/T22I

Plasmid

#1110

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

HSP82 with threonine 22 changed to isoleucine; re…

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
piHGpd/G81S

Plasmid

#1111

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

HSP82 with glycine 81 changed to serine; regulate…

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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piHGpd/G313S

Plasmid

#1114

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

HSP82 with glycine 313 changed to serine; regulat…

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
piHGpd/E381K

Plasmid

#1115

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

HSP82 with glutamic acid 381 changed to lysine; r…

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
piHGpd/A587T

Plasmid

#1116

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

HSP82 with alanine 587 changed to threonine; regu…

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
piHGpd/A41V

Plasmid

#1117

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

HSP82 with alanine 41 changed to valine; regulate…

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pEGFP Tensin1

Plasmid

#105297

Purpose

To study localization and function of Tensin1

Depositor

David Critchley, Kenneth Yamada

Article

Clark et al J Cell Biochem. 2010 Mar 1;109(4):808-17

Insert

Tensin1 (TNS1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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