We narrowed to 397 results for: SAM activators

1. pCS2TAL3-RR-zrif1_exon8

   Plasmid

   #194434

   Purpose

   Right (RR) TALEN for mutating zebrafish rif1 gene

   Depositor

   Christopher Sansam

   Article

   Masser et al eLife 2023 (Reviewed Preprint) 12:RP8767

   Insert

   zrif1_ex8_RIGHT (rif1 Zebrafish)
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   CMV IE94

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2TAL3-DD-zrif1_exon8

   Plasmid

   #194435

   Purpose

   Left (DD) TALEN for mutating zebrafish rif1 gene

   Depositor

   Christopher Sansam

   Article

   Masser et al eLife 2023 (Reviewed Preprint) 12:RP8767

   Insert

   zrif1_ex8_LEFT (rif1 Zebrafish)
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   CMV IE94

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC57-GATA1

   Plasmid

   #194436

   Purpose

   In Situ hybridization probes

   Depositor

   Christopher Sansam

   Article

   Masser et al eLife 2023 (Reviewed Preprint) 12:RP8767

   Insert

   GATA1a (gata1a Zebrafish)
   

   Use

   In situ hybridization

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPRa_all-in-one

   Plasmid

   #183695

   Purpose

   Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

   Depositor

   Edward Avezov

   Article

   Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZR043_Lenti-U6-sgEGFR-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

   Plasmid

   #180267

   Purpose

   Lentiviral expression vector for CRISPRa-SAM with EGFR targeting sgRNA

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   EGFR sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZR040_Lenti-U6-sgCtrl-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

   Plasmid

   #180266

   Purpose

   Lentiviral expression vector for CRISPRa-SAM with non-targeting control sgRNA

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   No-Target Control sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pZR047_Lenti-U6-sgGARP-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

   Plasmid

   #180268

   Purpose

   Lentiviral expression vector for CRISPRa-SAM with GARP targeting sgRNA

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   GARP sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG MCP-VP64

   Plasmid

   #92357

   Purpose

   CAG-driven ubiquitous expression of MCP-VP64. MCP is bacteriophage MS2 coat protein, VP64 is transcriptional activation domain. Part of SAM-mediated gene activation system in chicken embryos.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   MCP-VP64
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Raichu-OsRac1 CA

   Plasmid

   #133264

   Purpose

   Constitutively-active (CA) mutant of OsRac1; Acts as a positive control for small GTPases activation assays.

   Depositor

   Yoji Kawano

   Article

   Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

   Insert

   OsRac1(G19V) (LOC4325879 Oryza sativa)
   

   Use

   Tags

   CFP-lipid and Venus-CRIB

   Expression

   Plant

   Mutation

   OsRac1 G19V

   Promoter

   Ubi

   Available since

   Jan. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF-TAZ-N-Flag

    Plasmid

    #19025

    Depositor

    Michael Yaffe

    Article

    Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

    Insert

    TAZ (Wwtr1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Vector has no SV40 origin

    Promoter

    Available since

    Aug. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pEF-TAZ-N-Flag (S89A)

    Plasmid

    #19026

    Depositor

    Michael Yaffe

    Article

    Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

    Insert

    TAZ (Wwtr1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Vector has no SV40 origin, S89A (site directed mu…

    Promoter

    Available since

    Aug. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pGEX-mTAZ-WW (86)

    Plasmid

    #40915

    Depositor

    Michael Yaffe

    Article

    Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

    Insert

    TAZ (Wwtr1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains mTAZ 14-3-3 binding site and WW domain (…

    Promoter

    Tac

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pGEX-mTAZ-WW (43)

    Plasmid

    #40916

    Depositor

    Michael Yaffe

    Article

    Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

    Insert

    TAZ (Wwtr1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains mTAZ WW domain (43 amino acids, 122-164)

    Promoter

    Tac

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pEF-TAZ-N-Flag (-4)

    Plasmid

    #40914

    Depositor

    Michael Yaffe

    Article

    Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

    Insert

    TAZ (Wwtr1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Vector has no SV40 origin, and lacks the final fo…

    Promoter

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

15. A3RXB7

    Plasmid

    #177694

    Purpose

    expresses a N-acetyl hexosamine oxidase in E. coli

    Depositor

    Martin Engqvist

    Article

    Rembeza et al Chembiochem. 2022 Jan 19;23(2):e20210051

    Insert

    N-acetylhexosamine oxidase
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Raichu-OsRac1 WT

    Plasmid

    #133262

    Purpose

    Biosensor for monitoring activation for rice small GTPase OsRac1

    Depositor

    Yoji Kawano

    Article

    Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

    Insert

    OsRac1 (LOC4325879 Oryza sativa)
    

    Use

    Tags

    CFP-lipid and Venus-CRIB

    Expression

    Plant

    Mutation

    Promoter

    Ubi

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. RSV-Pit1

    Plasmid

    #24059

    Depositor

    Herbert Samuels, Frederick Stanley

    Article

    Fox et al Mol Endocrinol. 1990 Jul . 4(7):1069-80.

    Insert

    Pit1 (SLC20A1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

18. Raichu-OsRac1 DN

    Plasmid

    #133263

    Purpose

    Dominant-negative (DN) mutant of OsRac1; Acts as a negative control for small GTPases activation assays

    Depositor

    Yoji Kawano

    Article

    Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

    Insert

    OsRac1(T24N) (LOC4325879 Oryza sativa)
    

    Use

    Tags

    CFP-lipid and Venus-CRIB

    Expression

    Plant

    Mutation

    OsRac1 T24N

    Promoter

    Ubi

    Available since

    Jan. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-ABKAR(T/A)

    Plasmid

    #140052

    Purpose

    Negative control mutant for ABKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4

    Insert

    ABKAR(T/A)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available since

    Nov. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTMt_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

    Plasmid

    #101102

    Purpose

    Encodes dCas9(C)-VP64 with SAM components fused to transmembrane tether

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    TMt-dCas9(C)-VP64
    

    Use

    CRISPR and Synthetic Biology

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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