We narrowed to 2,233 results for: SE

1. pCZ47

   Plasmid

   #128414

   Purpose

   vab-1 rescuing minigene

   Depositor

   Andrew Chisholm

   Article

   George et al Cell. 1998 Mar 6;92(5):633-43.

   Insert

   vab-1 (vab-1 Nematode)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRegev_D3

   Plasmid

   #204182

   Purpose

   Single reporter with long guanine depleted (G-poor) region from splicing library.

   Depositor

   Oded Regev

   Article

   Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

   Insert

   Exon D3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Truncated CAG

   Available Since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRegev_S5

   Plasmid

   #204174

   Purpose

   Single reporter with secondary structure from splicing library.

   Depositor

   Oded Regev

   Article

   Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

   Insert

   Exon S5 in beta globin splicing minigene
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Truncated CAG

   Available Since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRegev_S1

   Plasmid

   #204150

   Purpose

   Single reporter with secondary structure from splicing library.

   Depositor

   Oded Regev

   Article

   Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

   Insert

   S1 exon in beta globin splicing minigene
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Truncated CAG

   Available Since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRegev_S4

   Plasmid

   #204170

   Purpose

   Single reporter with secondary structure from splicing library.

   Depositor

   Oded Regev

   Article

   Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

   Insert

   Exon S4 in beta globin splicing minigene
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Truncated CAG

   Available Since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRegev_S2

   Plasmid

   #204162

   Purpose

   Single reporter with secondary structure from splicing library.

   Depositor

   Oded Regev

   Article

   Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

   Insert

   S2 exon in beta globin splicing minigene
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Truncated CAG

   Available Since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJH805_pJH_LldR_Pcon-sfGFP.0TAG

   Plasmid

   #163736

   Purpose

   Constitutive expression of green fluorescent protein encoding zero stop codons

   Depositor

   Matthew Bennett

   Article

   Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

   Insert

   lldr gfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PX856

   Plasmid

   #62888

   Purpose

   C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Insert

   SpCas9 (aa536-1368)
   

   Use

   CRISPR

   Tags

   FKBP12, NLS, NLS SV40, and VP64

   Expression

   Mammalian

   Mutation

   Asparagine 863 to Alanine (N863A)

   Promoter

   CBh

   Available Since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. PX855

   Plasmid

   #62887

   Purpose

   N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Insert

   SpCas9 (aa 2-535)
   

   Use

   CRISPR

   Tags

   FRB and NES PTK2

   Expression

   Mammalian

   Mutation

   Aspartic acide 10 to Alanine (D10A)

   Promoter

   CBh

   Available Since

   March 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLSC-5

    Plasmid

    #62889

    Purpose

    Lenti vector for expression of inducible split Cas9

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Inserts

    SpCas9(574-1368)
    SpCas9(2-573)
    

    Use

    CRISPR and Lentiviral

    Tags

    FFBP12, FRB, NES PTK2, NLS SV40, and P2A

    Expression

    Mammalian

    Mutation

    *see comment below

    Promoter

    EFS

    Available Since

    March 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. SBE4-Luc

    Plasmid

    #16495

    Purpose

    Luciferase reporter containing four copies of the Smad binding site

    Depositor

    Bert Vogelstein

    Article

    Zawel et al Mol Cell. 1998 Mar . 1(4):611-7.

    Insert

    Smad binding element
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PP7 guide

    Plasmid

    #86168

    Purpose

    Expresses S. Pyogenes guide with PP7 aptamer in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

    Insert

    PP7 guide
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCEP4 Smad4

    Plasmid

    #16483

    Depositor

    Bert Vogelstein

    Article

    Riggins et al Nat Genet. 1996 Jul . 13(3):347-9.

    Insert

    Smad4 (SMAD4 Human)
    

    Use

    Tags

    Double HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pMIII-Neo

    Plasmid

    #153422

    Purpose

    MSCV with IRES-Neomycin resistant gene

    Depositor

    Maki Nakayama

    Article

    Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSP618

    Plasmid

    #169235

    Purpose

    Spear-ATAC lentiviral backbone - includes a mU6-sgRNA cassette with flanking Nextera sequencing adapters

    Depositor

    William Greenleaf

    Article

    Pierce et al Nat Commun. 2021 May 20;12(1):2969. doi:

    Insert

    mU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJH628_pJH_LldR_PlldR-sfGFP.3TAG

    Plasmid

    #163727

    Purpose

    Lactate-inducible expression of green fluorescent protein encoding three amber codons

    Depositor

    Matthew Bennett

    Article

    Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

    Insert

    lldr gfp.3TAG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    gfp.N39TAG,N135TAG,Y151TAG

    Promoter

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. iMbody_pSANG10-3F

    Plasmid

    #184496

    Purpose

    Expresses iMbody-6xHis-3xFLAG in BL21(DE3) bacteria

    Depositor

    Marcel Dinger

    Article

    Zeraati et al STAR Protoc. 2023 Nov 21;4(4):102729. do

    Insert

    iMbody
    

    Use

    Tags

    3xFLAG, 6xHis, and pelB leader

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MET-HYGRO

    Plasmid

    #218113

    Purpose

    Integration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.

    Depositor

    Carl Denard

    Article

    Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMSCVII-E2Crimson

    Plasmid

    #162756

    Purpose

    Retroviral (MSCV) expression of E2-Crimson fluorescent protein

    Depositor

    Maki Nakayama

    Article

    Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

    Insert

    E2-Crimson
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMIG-PP2A-Abeta

    Plasmid

    #15248

    Depositor

    William Hahn

    Article

    Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

    Insert

    PP2A regulatory subunit A, beta isoform (PPP2R1B Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only