We narrowed to 862 results for: golden gate assembly

1. GGE254

   Plasmid

   #120789

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   InsDown_Lip-NotI
   

   Use

   Cloning/assembly plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. GGE255

   Plasmid

   #120788

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   InsUp_Lip-NotI
   

   Use

   Cloning/assembly plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Prham-M.XbaI-M.EcoRI-M.SalI-p15A-aadA

   Plasmid

   #170767

   Purpose

   Methylase expression vector for 5RM assembly: expresses M.XbaI, M.EcoRI and M.SalI DNA methyltransferases in E. coli

   Depositor

   Ichiro Matsumura

   Article

   Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

   Inserts

   M.XbaI
   M.EcoRI
   M.SalI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rhamnose

   Available since

   Feb. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Prham-M.XhoI-M.MunI-M.Ocy1ORF8430P-p15A-aadA

   Plasmid

   #170768

   Purpose

   Methylase expression vector for 5RM assembly: expresses M.XhoI, M.MunI (MfeI) and M.Ocy1ORF8430P (SpeI) DNA methyltransferases in E. coli

   Depositor

   Ichiro Matsumura

   Article

   Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

   Inserts

   M.XhoI
   M.MunI
   M.Ocy1ORF8430P
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rhamnose

   Available since

   Feb. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. GGE207

   Plasmid

   #120792

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   InsDown-Mfe
   

   Use

   Cloning/assembly plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pXZ13lacZalpha

   Plasmid

   #160229

   Purpose

   places LacZalpha unit between Esp3I sites for better cutting of plasmid and ability to distinguish recombinants as white colonies when plated on Xgal plates

   Depositor

   Ian Duncan

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMC-Dest-BsaI

   Plasmid

   #200550

   Purpose

   Mini circle destination vector with BsaI sites for Golden Gate Assembly

   Depositor

   Junsu Kang

   Type

   Empty backbone

   Use

   Minicircle cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNDGG005

   Plasmid

   #231309

   Purpose

   Vector part for BEVA; BEVA2.0 Position 3 RSF1010 broad host range origin of replication with oriT, CmR.

   Depositor

   Barney Geddes

   Article

   Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

   Insert

   BEVA2.0 Position 3 RSF1010 broad host range origin of replication with oriT, CmR.
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNDGG006

   Plasmid

   #231310

   Purpose

   Vector part for BEVA; BEVA2.0 Position 3 p15A narrow host range origin of replication with oriT, CmR.

   Depositor

   Barney Geddes

   Article

   Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

   Insert

   BEVA2.0 Position 3 p15A narrow host range origin of replication with oriT, CmR.
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNDGG007

    Plasmid

    #231311

    Purpose

    Vector part for BEVA; BEVA2.0 Position 3 pMB1 narrow broad host range origin of replication with oriT, CmR.

    Depositor

    Barney Geddes

    Article

    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

    Insert

    BEVA2.0 Position 3 pMB1 narrow broad host range origin of replication with oriT, CmR.
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNDGG021

    Plasmid

    #231305

    Purpose

    Vector part for BEVA; BEVA2.0 Position 1 Level 1 BsaI cloning site without T0, AmpR

    Depositor

    Barney Geddes

    Article

    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

    Insert

    BEVA2.0 Position 1 Level 1 BsaI cloning site without T0, AmpR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNDGG002

    Plasmid

    #231308

    Purpose

    Vector part for BEVA; BEVA2.0 Position 2 Sp antibiotic resistance via aadA, AmpR/SpR

    Depositor

    Barney Geddes

    Article

    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

    Insert

    BEVA2.0 Position 2 Sp antibiotic resistance via aadA, AmpR/SpR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNDGG001

    Plasmid

    #231307

    Purpose

    Vector part for BEVA; BEVA2.0 Position 2 Km/Nm antibiotic resistance via nptII, CmR/KmR/NmR.

    Depositor

    Barney Geddes

    Article

    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

    Insert

    BEVA2.0 Position 2 Km/Nm antibiotic resistance via nptII, CmR/KmR/NmR.
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. L0I80_Bxb1mut(noBsaI)_GG1-GG4

    Plasmid

    #219715

    Purpose

    Level 0 Bxb1 integrase with mutated BsaI sites, GG1 and GG4 BsaI sites for golden gate assembly.

    Depositor

    Jennifer Nemhauser

    Insert

    GG1-Bxb1(noBsaI0)-GG4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNDGG010

    Plasmid

    #231315

    Purpose

    Vector part for BEVA; BEVA2.0 Position 4 attB recombinase site, AmpR. Also includes FRT EF module for L1 GG cloning.

    Depositor

    Barney Geddes

    Article

    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

    Insert

    BEVA2.0 Position 4 attB recombinase site, AmpR. Also includes FRT EF module for L1 GG cloning.
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNDGG022

    Plasmid

    #231306

    Purpose

    Vector part for BEVA; BEVA2.0 Position 1 Level 1 BsaI cloning site with alternate CAGA 3' fusion site, without T0, AmpR

    Depositor

    Barney Geddes

    Article

    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

    Insert

    BEVA2.0 Position 1 Level 1 BsaI cloning site with alternate CAGA 3' fusion site, without T0, AmpR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNDGG009

    Plasmid

    #231314

    Purpose

    Vector part for BEVA; BEVA2.0 Position 4 FRT recombinase site, AmpR. Also includes attB EF module for L1 GG cloning.

    Depositor

    Barney Geddes

    Article

    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

    Insert

    BEVA2.0 Position 4 FRT recombinase site, AmpR. Also includes attB EF module for L1 GG cloning.
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mTB3-Myc tag

    Plasmid

    #210205

    Purpose

    Type 3 phage-display vector that contains kanamycin resistance and displays the Myc-tag at the N-terminus of mature protein III.

    Depositor

    Brian Kay

    Article

    Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

    Insert

    Myc-tag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GGE177

    Plasmid

    #120766

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 1 (T1_Xpr2)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    T1_Xpr2
    

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GGE104

    Plasmid

    #120744

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Inducible promoter for gene expression in position1 (P1_EYK300-3AB)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    P1_EYK300-3AB
    

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge