We narrowed to 760 results for: mec 1 cells

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pCS2+ 3xFLAG-FEM1B L597A

Plasmid

#230994

Purpose

Transient overexpression of catalytic null mouse FEM1B in mammalian cells. Mutation abolishes interaction with CUL2.

Depositor

Michael Rape

Article

Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

Insert

FEM1B (Fem1b Mouse)

Tags

3xFLAG

Expression

Mammalian

Mutation

mutated Leu597 to Ala

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-EEA1

Plasmid

#109508

Purpose

Muscle specific zebrafish expression of mKate2 fused to early endosome associated 1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

EEA1 (eea1 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-SERCA1

Plasmid

#109498

Purpose

Muscle specific zebrafish expression of mKate2 fused to sarcoplasmic/endoplasmic calcium ATPase 1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

SERCA1 (atp2a1 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Mutation

Please see Depositor Comments

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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hTln1-R1R2

Plasmid

#191440

Purpose

Expresses the human TLN1 R1R2 domains in bacteria

Depositor

Ben Goult

Article

Gallego-Paez et al J Cell Biol. 2023 May 1;222(5):e20220901

Insert

Human Talin1 R1R2 domains (TLN1 Human)

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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Halo-TMD

Plasmid

#195347

Purpose

Fluorescent labeling for cell membranes

Depositor

Allen Liu

Article

Hsu et al ACS Sens. 2023 Jan 27;8(1):12-18. doi: 1

Insert

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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p3E-mScarlet

Plasmid

#140875

Purpose

Multisite Gateway middle entry vector for fusing mScarlet to the 3' end of an ORF.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mScarlet

Use

Gateway entry clone

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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LRP6-pCS2-VSVG

Plasmid

#27282

Depositor

Xi He

Article

Tamai et al Mol Cell. 2004 Jan 16. 13(1):149-56.

Insert

low density lipoprotein receptor-related protein 6 (LRP6 Human)

Use

Xenopus injection

Tags

VSVG

Available Since

March 3, 2011

Availability

Academic Institutions and Nonprofits only

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pME-Lifeact

Plasmid

#109545

Purpose

Multisite gateway vector for 5' tagging with the actin binding motif Lifeact

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actin binding motif Lifeact

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-EGFP

Plasmid

#140877

Purpose

126573: Multisite Gateway middle entry vector for fusing mScarlet to the 3' end of an ORF.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

EGFP

Use

Gateway entry clone

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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p5E-4xnrUAS

Plasmid

#87120

Purpose

Multisite gateway entry clone containing 4 non-repetitive UAS (upstream activation sequence) elements

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

4xnrUAS (non repetitive)

Use

Gateway vector

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pME-mScarlet-STOP

Plasmid

#140876

Purpose

Multisite Gateway middle entry vector for expression of untagged mScarlet.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mScarlet

Use

Gateway entry clone

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pME-Lifeact-mEos2-NS

Plasmid

#115549

Purpose

Multisite gateway vector for 5' tagging with Lifeact-mEos2. Used to make 115563. Parton lab clone IDA

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Lifeact-mEos2

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-CaaX(tH)

Plasmid

#109539

Purpose

Multisite gateway vector for 3' tagging with the CaaX domain from Hras

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CaaX domain from Hras

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pME-mScarlet-NS

Plasmid

#140874

Purpose

Multisite Gateway middle entry vector for fusing mScarlet to the 5' end of an ORF.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mScarlet

Use

Gateway entry clone

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-ATG18

Plasmid

#109556

Purpose

Multisite gateway vector for 3' tagging with yeast ATG18 (PLEKHA3). Binds PtdIns(3,5P)2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

yeast ATG18 (PLEKHA3)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pME-myristo

Plasmid

#109541

Purpose

Multisite gateway vector for 5' tagging with the 5' myristoylation sequence from ARF5

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

5' myristoylation sequence from ARF5

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-Dnm2a

Plasmid

#109571

Purpose

Multisite gateway vector for 3' tagging with Dynamin2a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Dynamin2a

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET23a-His-TEV-SpyCatcher3

Plasmid

#235149

Purpose

Bacterial expression of His-tagged SpyCatcher3 separated by a TEV protease recognition site.

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

SpyCatcher3

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p3E-Rab31

Plasmid

#109755

Purpose

Multisite gateway vector for 3' tagging with Rab31

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab31 (rab31 Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-Rab19

Plasmid

#109753

Purpose

Multisite gateway vector for 3' tagging with Rab19

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab19 (rab19 Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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