We narrowed to 594 results for: pFAST

1. pFastBac1_GI.1_Q141V-P221L

   Plasmid

   #162584

   Purpose

   Expresses norovirus GI.1 VP1 protein with mutations Q141V-P221L in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Glutamine 141 changed to Valine and Proline 221 c…

   Promoter

   polyhedrin

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFastBac1_GI.1_L144C-P221C

   Plasmid

   #162585

   Purpose

   Expresses norovirus GI.1 VP1 protein with mutations L144C-P221C in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Leucine 144 changed to Cysteine and Proline 221 c…

   Promoter

   polyhderin

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFastBac1_GI.1_G131C-N172C

   Plasmid

   #162586

   Purpose

   Expresses norovirus GI.1 VP1 protein with mutations G131C-N172C in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Glycine 131 changed to Cysteine and Asparagine 17…

   Promoter

   polyhedrin

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFastBac1_GI.1_N167C-L169C

   Plasmid

   #162587

   Purpose

   Expresses norovirus GI.1 VP1 protein with mutations N167C-L169C in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Asparagine 167 changed to Cysteine and Leucine 16…

   Promoter

   polyhedrin

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFast-dCry-V5His

   Plasmid

   #136240

   Purpose

   Protein expression of D. melanogaster (Dm) cytochrome with C-terminal V5 and His tags

   Depositor

   Aziz Sancar

   Article

   Ozturk et al Biochemistry. 2009 Sep 15;48(36):8585-93

   Insert

   DmCry
   

   Use

   Tags

   His and V5

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFastBac-GST-Mps1-KD

   Plasmid

   #114066

   Purpose

   protein production

   Depositor

   Geert Kops

   Article

   Jelluma et al PLoS One. 2008 Jun 11;3(6):e2415. doi: 1

   Insert

   Mps1-KD (TTK Human)
   

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   Mps1 KD (D664A)

   Promoter

   Available since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFastBac-GST-Mps1-WT

   Plasmid

   #114065

   Purpose

   protein production

   Depositor

   Geert Kops

   Article

   Jelluma et al PLoS One. 2008 Jun 11;3(6):e2415. doi: 1

   Insert

   Mps1-WT (TTK Human)
   

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   none

   Promoter

   Available since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Plekhm1 - C terminal pFastHFTb

   Plasmid

   #73829

   Purpose

   Express human Plekhm1 C-part in insect cells

   Depositor

   Paul Odgren

   Insert

   Plekhm1 (PLEKHM1 Human)
   

   Use

   Tags

   6xHis and Flag

   Expression

   Insect

   Mutation

   cloned C-terminal part of human Plekhm1 (aa 493- …

   Promoter

   Available since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

9. Plekhm1-N terminal pFastHFTb

   Plasmid

   #73828

   Purpose

   Express human Plekhm1 N-part in insect cells

   Depositor

   Paul Odgren

   Insert

   Plekhm1 (PLEKHM1 Human)
   

   Use

   Tags

   6xHis and Flag

   Expression

   Insect

   Mutation

   cloned N- terminal part of human Plekhm1 (aa 1- 4…

   Promoter

   Available since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pFastBac-Gm129-Flag

    Plasmid

    #53766

    Purpose

    baculovirus expression of Gm129

    Depositor

    Aziz Sancar

    Article

    Annayev et al J Biol Chem. 2014 Feb 21;289(8):5013-24.

    Insert

    Gm129 (Ciart Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pFastBac Dual α1B/β3 Tubulin

    Plasmid

    #170552

    Purpose

    Encodes codon-optimized human α1B and β3 tubulin for expression in and purification from insect cells.

    Depositor

    Tarun Kapoor

    Article

    Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

    Inserts

    TUBA1B (TUBA1B Human)
    TUBB3 (TUBB3 Human)
    

    Use

    Tags

    AP linker, GS linker (GGSGG), His10 tag, L21 enha…

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFastBac Dual wt p110α/p85α

    Plasmid

    #199302

    Purpose

    co-expression of His-tagged p110α wt and Avi-tagged p85α wt proteins

    Depositor

    Peter Vogt

    Article

    Favalli et al Nat Chem. 2021 Jul;13(7):714. doi: 10.10

    Insert

    His-tagged p110α wt and Avi-tagged p85α wt (PIK3CA, PIK3R1 Human)
    

    Use

    Tags

    Avi and His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFastBac_HT_B_6xHis-TEV-GFP-NBR1

    Plasmid

    #190928

    Purpose

    Plasmid for the expression and purification of GFP labelled NBR1. Internal reference: SMC 914

    Depositor

    Sascha Martens

    Article

    Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

    Insert

    NBR1 (NBR1 Human)
    

    Use

    Tags

    Strep-TEV-GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFastBac1-OSF-TRIM5alpha(human)

    Plasmid

    #79034

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(human) (TRIM5 Human)
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    March 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFastBac LIC cloning vector (4A)

    Plasmid

    #30111

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFastBac-GST-DAXX-His

    Plasmid

    #175781

    Purpose

    DAXX protein for baculovirus expression system

    Depositor

    Xiaolu Yang

    Article

    Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

    Insert

    death domain associated protein (DAXX Human)
    

    Use

    Tags

    GST and TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFastbac-Dual-StrepII-BARD1

    Plasmid

    #137166

    Purpose

    Expresses human StrepII-tagged BARD1 for purification from baculovirus/insect cell system

    Depositor

    Andrew Deans

    Article

    Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

    Insert

    BARD1 (BARD1 Human)
    

    Use

    Tags

    StrepII

    Expression

    Insect

    Mutation

    fully synthetic, codon optimized for insect expre…

    Promoter

    p10

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFastbacHTB HA-human PP2A Cα D88N

    Plasmid

    #132633

    Purpose

    expresses HA-human PP2A Cα D88N in Hi5 insect cell

    Depositor

    Wenqing Xu

    Article

    Cho et al Nature. 2007 Jan 4;445(7123):53-7. doi:

    Insert

    PP2A Cα D88N
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFastBac (GST-SNAP-Shp2)

    Plasmid

    #177897

    Purpose

    Baculo expression of GST tag fused to SNAP tag and human Shp2

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    Shp2 (PTPN11 Human)
    

    Use

    Tags

    TEV-GST-PreScission cut site-SNAP tag

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Dec. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFastBac(His10-SNAP-Csk)

    Plasmid

    #177895

    Purpose

    Baculo expression of His10 tag fused to SNAPtag and human Csk

    Depositor

    Ron Vale

    Article

    Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

    Insert

    Csk (CSK Human)
    

    Use

    Tags

    10xHis-TEV-TVMV-SNAP tag

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge