We narrowed to 3,131 results for: actin

1. pCMV5 BRGI-Flag

   Plasmid

   #19143

   Depositor

   Joan Massague

   Article

   Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

   Insert

   SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pExpreS2-2

   Plasmid

   #175362

   Purpose

   G418R backbone for recombinant protein overexpression with Drosophila ExpreS2 Platform

   Depositor

   ExpreS2ion Biotechnologies

   Article

   Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Fused Actin-HSP70

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. mKate1.31-Lifeact-7

   Plasmid

   #54668

   Purpose

   Localization: Actin, Excitation: 588, Emission: 635

   Depositor

   Michael Davidson

   Article

   Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

   Type

   Empty backbone

   Use

   Tags

   LifeAct-mKate1.31

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. SMA-Cherry

   Plasmid

   #110209

   Purpose

   Mapping of mesenchymal progenitor cells.

   Depositor

   David Rowe

   Article

   Grcevic et al Stem Cells. 2012 Feb;30(2):187-96. doi:

   Insert

   mCherry
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   Smooth muscle alpha-actin and first intron

   Available since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAc-sgRNA-Cas9

   Plasmid

   #49330

   Purpose

   Expresses sgRNA and Cas9-Puro in Drosophila S2 cells

   Depositor

   Ji-Long Liu

   Article

   Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

   Inserts

   Cas9
   dU6-sgRNA
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Insect

   Mutation

   Human codon optimised

   Promoter

   Actin-5c and Drosophila U6

   Available since

   Dec. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. TagRFP-T_A1aY1

   Plasmid

   #158751

   Purpose

   Lentiviral vector for expression of TagRFP-T_A1aY1 in mammalian cells for detection of tyrosinated microtubules.

   Depositor

   Minhajuddin Sirajuddin

   Article

   Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

   Insert

   TagRFP-T_A1aY1
   

   Use

   Lentiviral

   Tags

   TagRFP-T

   Expression

   Mammalian

   Mutation

   Promoter

   CAG (CMV enhancer and chicken beta actin promoter)

   Available since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUB_smFLAG_ActB_MS2

   Plasmid

   #81083

   Purpose

   Translation reporter: Encodes Beta-actin (374 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).

   Depositor

   Tim Stasevich

   Article

   Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

   Insert

   smFLAG_ActB_24xMS2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS SARS-CoV-2 XBB Spike

   Plasmid

   #195287

   Purpose

   Expresses codon-optimized full length SARS-CoV-2 XBB Spike

   Depositor

   Marceline Côté

   Insert

   SARS-CoV-2 XBB Spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains the following mutations: T19I, LPPA24-27…

   Promoter

   chicken β-actin promoter, CMV enhancer

   Available since

   Feb. 8, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. dCas9-10xSunTag(22aa)

   Plasmid

   #215745

   Purpose

   Expresses dCas9 with 10 GCN4 epitopes separated by 22 amino acid linkers

   Depositor

   Albert Jeltsch

   Article

   Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

   Insert

   dCas9-10xSunTag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   chicken beta-actin promoter

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Dronpa-Lifeact-7

    Plasmid

    #54740

    Purpose

    Localization: Actin, Excitation: 503, Emission: 518

    Depositor

    Michael Davidson

    Article

    Rego et al Proc Natl Acad Sci U S A. 2012 Jan 17;10

    Type

    Empty backbone

    Use

    Tags

    Dronpa-Lifeact

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. TagBFP_A1aY1

    Plasmid

    #158752

    Purpose

    Lentiviral vector for expression of TagBFP_A1aY1 in mammalian cells for detection of tyrosinated microtubules.

    Depositor

    Minhajuddin Sirajuddin

    Article

    Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

    Insert

    TagBFP-T_A1aY1
    

    Use

    Lentiviral

    Tags

    TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG (CMV enhancer and chicken beta actin promoter)

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBa.GFP-KIF5B

    Plasmid

    #134625

    Purpose

    Mammalian expression of mouse KIF5B 376-963

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF5B (Kif5b Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 377 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZac2.1 gfaABC1D-LifeAct-GFP

    Plasmid

    #190199

    Purpose

    Expresses the small actin binding peptide, LifeAct, with a GFP reporter

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    LifeAct-eGFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    gfaABC1D

    Available since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB

    Plasmid

    #52889

    Purpose

    "CoinFLP-Gal4" - Expresses Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express Gal4, or FRT3-FRT3 pair to prevent Gal4 expression

    Depositor

    Iswar Hariharan

    Article

    Bosch et al Development. 2015 Feb 1;142(3):597-606.

    Insert

    Gal4 (GAL4 Budding Yeast)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pclbw-eGFPomp25

    Plasmid

    #58424

    Purpose

    Mammalian expression of GFP targeted to the mitochondrial outer membrane

    Depositor

    David Chan

    Article

    Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

    Insert

    eGFP-omp25 (Synj2bp Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains aa's 109-145 of mouse omp25

    Promoter

    CMV/b-actin

    Available since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pCI H2B-RFP

    Plasmid

    #92398

    Purpose

    Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-mRFP1 reporter.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    IRES H2B RFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-Cas13d-T2A-GFP(1-10)

    Plasmid

    #224566

    Purpose

    Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and split GFP(1-10) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    Cas13d-T2A-GFP(1-10)
    

    Use

    CRISPR

    Tags

    T2A-split GFP(1-10)

    Expression

    Mutation

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCI H2B-GFP

    Plasmid

    #92399

    Purpose

    Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-EGFP reporter.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    IRES H2B EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Bxb1-GA_CAG-ITPKA-3x-mScarlet-I

    Plasmid

    #229782

    Purpose

    Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of a mScarlet-I F-actin reporter (3 copies)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    3x mScarlet-I
    

    Use

    Synthetic Biology

    Tags

    ITPKA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. E407V Nuclear Myosin I YFP

    Plasmid

    #60242

    Purpose

    Expresses a E407V mutant of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding and motor activities

    Depositor

    Primal de Lanerolle

    Article

    Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

    Insert

    Nuclear Myosin 1 (Myo1c Mouse)
    

    Use

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Mutation

    E407V

    Promoter

    Available since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only