Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 7,945 results for: Lif

Showing: 1581 - 1600 of 7945 results

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NmMetNI-WT

Plasmid

#172114

Purpose

Expression of MetN and MetI membrane proteins

Depositor

Douglas Rees

Article

Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:

Inserts

MetN
MetI

Tags

10x His Tag and Enterokinase

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-WIPI2dR108E-cs-TEV-STREP

Plasmid

#171835

Purpose

Mammalian expression of human WIPI2d R108E with C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Tags

Strep

Expression

Mammalian

Mutation

R108E

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-WIPI2dR125E-cs-TEV-STREP

Plasmid

#171836

Purpose

Mammalian expression of human WIPI2d R125E with C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Tags

Strep

Expression

Mammalian

Mutation

R125E

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-WIPI2dK128E-cs-TEV-STREP

Plasmid

#171837

Purpose

Mammalian expression of human WIPI2d K128E with C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Tags

Strep

Expression

Mammalian

Mutation

K128E

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-WIPI2dH85E-cs-TEV-STREP

Plasmid

#171831

Purpose

Mammalian expression of human WIPI2d H85E with C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Tags

Strep

Expression

Mammalian

Mutation

H85E

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-WIPI2dK88E-cs-TEV-STREP

Plasmid

#171832

Purpose

Mammalian expression of human WIPI2d K88E with C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Tags

Strep

Expression

Mammalian

Mutation

K88E

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-WIPI2dI92E-cs-TEV-STREP

Plasmid

#171833

Purpose

Mammalian expression of human WIPI2d I92E with C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Tags

Strep

Expression

Mammalian

Mutation

I92E

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-WIPI2dC93E-cs-TEV-STREP

Plasmid

#171834

Purpose

Mammalian expression of human WIPI2d C93E with C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Tags

Strep

Expression

Mammalian

Mutation

C93E

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B S395E

Plasmid

#176006

Purpose

Expresses WIPI2B (S395E) fused to Halo in mammalian cells

Depositor

Erika Holzbaur

Article

Stavoe et al Elife. 2019 Jul 16;8. pii: 44219. doi: 1

Insert

WIPI2B (S395E) (WIPI2 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

Serine 395 to Glutamate

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B S395A

Plasmid

#176005

Purpose

Expresses WIPI2B (S395A) fused to Halo in mammalian cells

Depositor

Erika Holzbaur

Article

Stavoe et al Elife. 2019 Jul 16;8. pii: 44219. doi: 1

Insert

WIPI2B (S395A) (WIPI2 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

Serine 395 to Alanine

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLIC-lyn11-myc-LOV2GIVe (L)

Plasmid

#170624

Purpose

For the mammalian expression of mLOV2GIVe bearing the lit-mimicking mutation I539E

Depositor

Mikel Garcia-Marcos

Article

Garcia-Marcos et al Elife. 2020 Sep 16;9. pii: 60155. doi: 1

Insert

Lyn11-myc-LOV2GIVe (L)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pSUMO chicken-cPLA2-C2-domain

Plasmid

#170753

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Hirano et al Elife. 2019 May 3;8. pii: 44760. doi: 10

Insert

PLA2G4A-C2 domain (PLA2G4A Chicken)

Tags

SUMO

Expression

Bacterial

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2bleo-Nectin-2

Plasmid

#170295

Purpose

A knock-out vector for the mouse Nectin-2

Depositor

Michiyuki Matsuda

Article

Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

Insert

A gRNA targeting the mouse Nectin-2 gene.

Use

CRISPR and Lentiviral

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2bleo-Necl-5

Plasmid

#170294

Purpose

A knock-out vector for the mouse Necl-5

Depositor

Michiyuki Matsuda

Article

Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

Insert

A gRNA targeting the mouse Necl-5 gene.

Use

CRISPR and Lentiviral

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pLIC-lyn11-myc-LOV2GIVe (D)

Plasmid

#170625

Purpose

For the mammalian expression of mLOV2GIVe dark-mimicking mutation C450S

Depositor

Mikel Garcia-Marcos

Article

Garcia-Marcos et al Elife. 2020 Sep 16;9. pii: 60155. doi: 1

Insert

Lyn11-myc-LOV2GIVe (D)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pJCC_084 Cas12i1 D647A

Plasmid

#171671

Purpose

For bacterial expression of Cas12i1 D647A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

Cas12i1 D647A

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed aspartate 647 to alanine

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-TK-Firefly-cjCD69

Plasmid

#122279

Purpose

Lentiviral vector for stable expression of Firefly luciferase.

Depositor

Demián Cazalla

Article

Gorbea et al Elife. 2019 Sep 20;8. pii: 50530. doi: 1

Inserts

Firefly luciferase
cjCD69 3'UTR

Use

Lentiviral and Luciferase

Expression

Mammalian

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-TK-Firefly-cjMGA

Plasmid

#122280

Purpose

Lentiviral vector for stable expression of Firefly luciferase.

Depositor

Demián Cazalla

Article

Gorbea et al Elife. 2019 Sep 20;8. pii: 50530. doi: 1

Inserts

Firefly luciferase
cjMGA 3'UTR

Use

Lentiviral and Luciferase

Expression

Mammalian

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-TK-Firefly-cjPACS1

Plasmid

#122281

Purpose

Lentiviral vector for stable expression of Firefly luciferase.

Depositor

Demián Cazalla

Article

Gorbea et al Elife. 2019 Sep 20;8. pii: 50530. doi: 1

Inserts

Firefly luciferase
cjPACS1 3'UTR

Use

Lentiviral and Luciferase

Expression

Mammalian

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-TK-Firefly-cjSTK4

Plasmid

#122282

Purpose

Lentiviral vector for stable expression of Firefly luciferase.

Depositor

Demián Cazalla

Article

Gorbea et al Elife. 2019 Sep 20;8. pii: 50530. doi: 1

Inserts

Firefly luciferase
cjSTK4 3'UTR

Use

Lentiviral and Luciferase

Expression

Mammalian

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 1581 - 1600 of 7945 results

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