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We narrowed to 4,839 results for: pcr

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Showing: 1581 - 1600 of 4839 results

  1. pEM705 Gsi (Ser)

    Plasmid
    #109352
    Purpose
    GsX chimera with Gαi C-terminus (Pertussis toxin insensitive mutant)
    Depositor
    Robert Lucas
    Publication
    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gsi chimera
    Tags
    None
    Expression
    Mammalian
    Mutation
    Changed Cys at position 391 to Serine (Pertussis …
    Promoter
    CAG
    Available since
    May 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    109352-plasmid-map-sequence-id-210153

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  2. S1PR5-Tango

    Plasmid
    #66500
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S1PR5 (S1PR5 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  5. LHCGR-Tango

    Plasmid
    #66417
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LHCGR (LHCGR Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. pcDNA5/FRT/TO-GAlphaoA-RLuc8

    Plasmid
    #140976
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GAlphaoA-RLuc8 (GNAO1 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    140976-plasmid-map-sequence-id-300659

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  7. pGJJ055

    Plasmid
    #183622
    Purpose
    ddPCA mutagenesis plasmid - Efficient mutant library cloning plasmid suitable for oligo synthesis, error prone PCR or nicking mutagenesis strategies.
    Depositor
    Ben Lehner
    Publication
    Faure et al Nature. 2022 Apr;604(7904):175-183. doi:
    Type
    Empty backbone
    Expression
    Bacterial
    Available since
    May 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    183622-plasmid-map-sequence-id-361477

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  8. pGC(2.1)-U6.3

    Plasmid
    #170516
    Purpose
    PCR template vector for amplifying gRNAcore(2.1)-pU6.3 promoter fragment; used together with pAC-CR7T-gRNA2.1-nlsBFP
    Depositor
    Chun Han
    Publication
    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNAcore(2.1)-pU6.3
    Use
    Pcr template vector for amplifying grnacore(2.1)-…
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    170516-plasmid-map-sequence-id-334635

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  11. pcDNA3-HLA-cMyc-EcopT1R1

    Plasmid
    #113962
    Purpose
    mammalian expression plasmid for c-myc-tagged E. coli codon-optimized human T1R1 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain
    Depositor
    Erik Procko
    Publication
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    T1R1 (TAS1R1 Human)
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    truncate N-terminal 24 residues
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113962-plasmid-map-sequence-id-223994

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  12. pcDNA3.1(+)-HLA-cmyc-optiT1R3 a21-852

    Plasmid
    #113949
    Purpose
    mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain
    Depositor
    Erik Procko
    Publication
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    T1R3 (TAS1R3 Human)
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    residues 21-852
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113949-plasmid-map-sequence-id-224194

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  13. pAAV-hSyn-GRAB_DA1h (AAV Retrograde)

    Viral prep
    #113050-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-GRAB_DA1h (#113050). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_DA1h plasmid DNA. Synapsin-driven expression of GRAB-DA1h dopamine sensor in neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Yulong Li
    Publication
    Sun et al Cell. 2018 Jul 12;174(2):481-496.e19. do
    Promoter
    Syn
    Available since
    Nov. 4, 2019
    Availability
    Academic Institutions and Nonprofits only

  14. pAAH4

    Plasmid
    #41828
    Purpose
    Used as a PCR template for tagging yeast proteins with the DHFR tag by homologous recombination and selection for phleomycin resistance
    Depositor
    Melissa Moore
    Publication
    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.
    Type
    Empty backbone
    Use
    Pcr template
    Expression
    Yeast
    Promoter
    TEF
    Available since
    Oct. 29, 2013
    Availability
    Academic Institutions and Nonprofits only
    41828-plasmid-map-sequence-id-69199

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  16. pBP-lacZ

    Plasmid
    #72948
    Purpose
    Entry vector for Bioparts - Oligo anneal or PCR with internal BsaI sites at 5' and 3' ends using NdeI/SphI cloning
    Depositor
    Paul Freemont
    Publication
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Level 0 - Bioparts
    Use
    Synthetic Biology
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    72948-plasmid-map-sequence-id-133793

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  17. pJW2072

    Plasmid
    #154334
    Purpose
    Crispr repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Jordan Ward
    Publication
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    30aa linker::mScarlet-I (dpi)::3xMyc::10aa linker
    Use
    CRISPR
    Expression
    Worm
    Mutation
    Silent mutations to remove piRNA sites
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    154334-plasmid-map-sequence-id-318659

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  19. S17-1λpir gyrAR462C

    Bacterial strain
    #237425
    Purpose
    This engineered E. coli S17-1 λpir strain, featuring a mutation in the gyrA gene (462Arg→Cys), was designed to confer resistance to the CcdB toxin, allowing it to survive with a ccdB-carrying plasmid.
    Depositor
    Xue Liu
    Publication
    Zhang et al Nat Commun. 2025 Jul 1;16(1):5870. doi:
    Bacterial resistance
    None
    Available since
    Aug. 7, 2025
    Availability
    Academic Institutions and Nonprofits only

  20. pJW2172

    Plasmid
    #163096
    Purpose
    CRISPR repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Jordan Ward
    Publication
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    30aa linker::mNeonGreen (dpi)::3xFLAG::10aa linker
    Use
    CRISPR
    Expression
    Worm
    Mutation
    Silent mutations to remove piRNA sites
    Available since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163096-plasmid-map-sequence-id-320618

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Showing: 1581 - 1600 of 4839 results