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pKLxR5

Plasmid

#149465

Purpose

Broad-Host range expression vector, RK2 and pUC origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-FKBP(M)x4

Plasmid

#85003

Purpose

Expresses EGFP-FKBP(M)x4 in yeast

Depositor

Benjamin Glick

Article

Barrero et al Cell Logist. 2016 Jun 24;6(3):e1204848.

Insert

EGFP-FKBP(M)x4

Expression

Yeast

Available Since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pWT016i

Plasmid

#96856

Purpose

theophylline-agRNA-GFP

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

theophylline-agRNA-GFP

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pAd5-B3

Plasmid

#122553

Purpose

Block 3 for AdenoBuilder genome assembly

Depositor

Fred Bunz

Article

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 10862-14499

Use

Synthetic Biology

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVK_AE(J23100-RiboJ-UTR1-E0040m-B0015)

Plasmid

#197662

Purpose

Expresses GFP under the control of J23100 and RiboJ-UTR1

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pPEE13

Plasmid

#128359

Purpose

Recycable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mCherry ORF and a flexable linker at the 5' end.

Depositor

James Fraser

Article

Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

Insert

Acetamidase (amdS)

Use

Unspecified

Promoter

TEF1

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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TS_pLuxLac

Plasmid

#140426

Purpose

Toggle switch circuit responsive to AHL gradient.

Depositor

Yolanda Schaerli

Article

Barbier et al Mol Syst Biol. 2020 Jun;16(6):e9361. doi

Inserts

GFP
LacI
TetR
mCherry

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 15, 2020

Availability

Academic Institutions and Nonprofits only

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pFLxR5

Plasmid

#149482

Purpose

Broad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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6798 pJC25NMstop

Plasmid

#1228

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

NM (SUP35 Budding Yeast)

Expression

Bacterial

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA-LUCI-GECO1

Plasmid

#113675

Purpose

Genetically-encoded ratiometric bioluminescent Ca2+ indicator based on GCaMP6s

Depositor

Robert Campbell

Article

Qian et al Chembiochem. 2018 Jun 22. doi: 10.1002/c

Insert

LUCI-GECO1

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pFVK5

Plasmid

#167522

Purpose

Broad-Host range expression vector, RSF1010 origin, VanRAM repressor, PVanCC promoter, mKelly2, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

VanRAM-mKelly2

Use

Synthetic Biology

Expression

Bacterial

Promoter

PVanCC

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAd5-B6

Plasmid

#122556

Purpose

Block 6 for AdenoBuilder genome assembly

Depositor

Fred Bunz

Article

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 25043-30907

Use

Synthetic Biology

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pTEC16

Plasmid

#30175

Depositor

Lalita Ramakrishnan

Article

Takaki et al Nat Protoc. 2013 Jun;8(6):1114-24. doi:

Insert

Mycobacterium Strong Promoter (MSP)

Use

Mycobacteria expression

Tags

mOrange2

Available Since

June 3, 2011

Availability

Academic Institutions and Nonprofits only

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pFCiR5

Plasmid

#149479

Purpose

Broad-Host range expression vector, RSF1010 origin, CinR(AM) activator, PCin promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CinR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCin

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVK_AE(J23106-RiboJ-UTR1-E0040m-B0015)

Plasmid

#197674

Purpose

Expresses GFP under the control of J23106 and RiboJ-UTR1

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVK_AE(J23100-RiboJ-BCD2-E0040m-B0015)

Plasmid

#197661

Purpose

Expresses GFP under the control of J23100 and RiboJ-BCD2

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVK_AE(J23103-RiboJ-BCD2-E0040m-B0015)

Plasmid

#197667

Purpose

Expresses GFP under the control of J23103 and RiboJ-BCD2

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pWVR5

Plasmid

#167509

Purpose

Broad-Host range expression vector, pSa origin, VanRAM repressor, PVanCC promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

VanRAM-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PVanCC

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pFTC1

Plasmid

#64951

Purpose

source of TcR FRT cassette

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

FRT-TcR-FRT

Expression

Bacterial

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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6975 pPROEX-HTb-SIS1

Plasmid

#1233

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

sis1 (SIS1 Budding Yeast)

Tags

his tag (tev cleavable)

Expression

Bacterial

Available Since

July 18, 2005

Availability

Academic Institutions and Nonprofits only