We narrowed to 5,091 results for: codon optimized

1. pHH0103 ARHGAP27 WW domain #1

   Plasmid

   #104282

   Purpose

   Bacterial expression of WW domain #1 from ARHGAP27

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   ARHGAP27 WW domain #1 (ARHGAP27 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHH0103 ARHGAP27 WW domain #2

   Plasmid

   #104283

   Purpose

   Bacterial expression of WW domain #2 from ARHGAP27

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   ARHGAP27 WW domain #2 (ARHGAP27 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHH0103 PDZK10 WW domain

   Plasmid

   #104285

   Purpose

   Bacterial expression of WW domain from PDZK10

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   PDZK10 WW domain (FRMPD4 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHH0103 KIAA0082 WW domain

   Plasmid

   #104288

   Purpose

   Bacterial expression of WW domain from KIAA0082

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   KIAA0082 WW domain (CMTR1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHH0103 DiGeorge WW domain

   Plasmid

   #104292

   Purpose

   Bacterial expression of WW domain from DiGeorge

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   DiGeorge WW domain (DGCR8 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHH0103 FNBP4 WW domain #1

   Plasmid

   #104263

   Purpose

   Bacterial expression of WW domain #1 from FNBP4

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   FNBP4 WW domain #1 (FNBP4 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHH0103 FNBP4 WW domain #2

   Plasmid

   #104264

   Purpose

   Bacterial expression of WW domain #2 from FNBP4

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   FNBP4 WW domain #2 (FNBP4 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHH0103 DRP2 WW domain

   Plasmid

   #104270

   Purpose

   Bacterial expression of WW domain from DRP2

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   DRP2 WW domain (DRP2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHH0103 HECW2 WW domain #1

   Plasmid

   #104244

   Purpose

   Bacterial expression of WW domain #1 from HECW2

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   HECW2 WW domain #1 (HECW2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available since

   Feb. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MAST4

    Plasmid

    #38998

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MAST4 (MAST4 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    codon-optimized

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

11. MAST1

    Plasmid

    #39065

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MAST1 (MAST1 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    codon-optimized

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)

    Plasmid

    #107942

    Purpose

    Mammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutions

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)
    

    Use

    Tags

    3x HA and NLS (nucleoplasmin)

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R, and N282A

    Promoter

    CAG

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

    Plasmid

    #107943

    Purpose

    Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
    

    Use

    Tags

    3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R and D908A

    Promoter

    CAG

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti CMV Puro DEST (w118-1) SDC3

    Plasmid

    #107501

    Purpose

    Expresses SDC3, puromycin resistant

    Depositor

    Alejandro Gutierrez

    Article

    Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

    Insert

    SDC3 (codon optimized) (SDC3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Codon optimized sequence

    Promoter

    CMV

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV uN2CpolyGly op GFP (NIID)

    Plasmid

    #224356

    Purpose

    Express GFP tagged upsteram ORF of NOTCH2NLC with an expansion (100x) of GGC repeats with codon optimization (NOT pure GGC repeats)

    Depositor

    Nicolas Charlet-Berguerand

    Article

    Boivin et al Neuron. 2021 Jun 2;109(11):1825-1835.e5.

    Insert

    uN2C with a GGC repeat expansion (100x)
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Codon-optimized for expression in human, so no pu…

    Promoter

    CMV + chimeric intron (CAG)

    Available since

    Feb. 5, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pET-28b-T7-henAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1935)

    Plasmid

    #114073

    Purpose

    T7 promoter bacterial expression plasmid for human codon optimized enAsCas12a-HF1(E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag
    

    Use

    Tags

    NLS(nucleoplasmin) and 6xHis

    Expression

    Bacterial

    Mutation

    E174R, S542R, K548R and N282A

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTol2-UAS-zArchon1-KGC-EGFP-ER2

    Plasmid

    #108427

    Purpose

    Codon-optimized Archon1 fluorescent voltage reporter in zebrafish expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    zArchon1-KGC-EGFP-ER2
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    UAS

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCJ208

    Plasmid

    #162681

    Purpose

    Lidded variant of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) removing a seven-residue loop of PETase (Trp185:Phe191, PETase numbering) and replacing it with Gly251:Thr472 from MHETase; codon optimized for expression in E. coli K12, with C-terminal His tag.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) incorporating the MHETase lid
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Replacement of W185-F191 with G251-T472 from MHET…

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.4) (RTW1028)

    Plasmid

    #114084

    Purpose

    CAG promoter expression plasmid for human codon optimized enAsBE1.4

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.4)
    

    Use

    Tags

    2x SV40 NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R and D908A, human codon optimi…

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCJ217

    Plasmid

    #162685

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating two point mutations, G489C and S530C, to introduce a 6th disulfide bond (from PETase).

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with mutations to introduce a 6th disulfide bond from PETase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    G489C, S530C; codon optimized for E. coli K12

    Promoter

    T7/lac

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge