We narrowed to 5,135 results for: codon optimized

1. pET28a(+)v2_TwinStrep-SUMO-FLP-8xHis

   Plasmid

   #246980

   Purpose

   Expresses E. coli codon-optimized TwinStrep-SUMO-tagged FLP recombinase with N- and C-terminal NLS, using an improved pET design from Shilling et al. 2020.

   Depositor

   Amro Hamdoun

   Insert

   FLP Recombinase
   

   Use

   Synthetic Biology

   Tags

   8xHis, Nucleoplasmin, SUMO, SV40, and TwinStrep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. DBJS-p2.11

   Plasmid

   #246891

   Purpose

   A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid encoding guide for G3BP1 Nterm.

   Depositor

   David Bartel

   Insert

   G3BP1 Nterm Guide RNA 1 (G3BP1 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. DBJS-p2.16

   Plasmid

   #246893

   Purpose

   A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid encoding guide for CAPRIN1 Nterm.

   Depositor

   David Bartel

   Insert

   Caprin1 Nterm Guide RNA 1 (CAPRIN1 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a(+)v2_TwinStrep-SUMO-φC31(PhiC31)-8xHis

   Plasmid

   #246982

   Purpose

   Expresses E. coli codon-optimized TwinStrep-SUMO-tagged φC31(PhiC31) integrase with N- and C-terminal NLS, using an improved pET design from Shilling et al. 2020.

   Depositor

   Amro Hamdoun

   Insert

   φC31(PhiC31)
   

   Use

   Synthetic Biology

   Tags

   8xHis, Nucleoplasmin, SUMO, SV40, and TwinStrep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSG1017-2xBsaI-SaKKH-2xUGI_P2A_EGFP

   Plasmid

   #239450

   Purpose

   Codon-optimized S. aureus KKH Cas9 with 2x UGI. 2x BsaI sites for easy cloning of varying deaminases for cytosine base editing. EGFP can serve as a transfection marker.

   Depositor

   Alexis Komor

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSG1040-rA1-SaKKH-2xUGI_P2A_EGFP

   Plasmid

   #239463

   Purpose

   Cytosine base editor with codon-optimized S. aureus KKH Cas9, rAPOBEC1 deaminase, and 2x UGI. EGFP can be used as a transfection marker.

   Depositor

   Alexis Komor

   Insert

   S.a. rA1 CBE 2xUGI
   

   Use

   CRISPR

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR-ABCC1_K684I_STOP

   Plasmid

   #221430

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Insert

   ABCC1 (ABCC1 Human)
   

   Expression

   Mammalian

   Mutation

   K684I

   Available Since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR-ABCC1_S769N_STOP

   Plasmid

   #221431

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Insert

   ABCC1 (ABCC1 Human)
   

   Expression

   Mammalian

   Mutation

   S769N

   Available Since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCCL-c-MNDU3-CD3D c.202C>T-cDNA

   Plasmid

   #198022

   Purpose

   LV Expression of CD3D cDNA with mutation at c.202C>T used as a disease editing target for CD3d SCID mutaiton identified in Mennonite population

   Depositor

   Donald Kohn

   Insert

   CD3D c.202C>T-cDNA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Codon optimized (20 bp) on either end of cDNA for…

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCCL-c-MNDU3-CD3D-275-2A>G

    Plasmid

    #198024

    Purpose

    LV Expression of CD3D cDNA with mutation at c.275-2A>G used as a disease editing target for CD3d SCID mutation identified in Japan

    Depositor

    Donald Kohn

    Insert

    CD3D c.275-2A>G
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Codon optimized (20 bp) on either end of cDNA for…

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Cas9_P2A_T4pol

    Plasmid

    #190146

    Purpose

    Expresses SpCas9 and T4pol

    Depositor

    Timothy Lu

    Article

    Nakade et al bioRxiv 2022.12.05.518807

    Insert

    SpCas9_P2A_T4pol
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Codon Optimized for mammalian cells

    Promoter

    CBh

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHH0103 UTROPHIN WW domain

    Plasmid

    #104275

    Purpose

    Bacterial expression of WW domain from UTROPHIN

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    UTROPHIN WW domain (UTRN Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHH0103 ARHGAP27 WW domain #1

    Plasmid

    #104282

    Purpose

    Bacterial expression of WW domain #1 from ARHGAP27

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    ARHGAP27 WW domain #1 (ARHGAP27 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHH0103 ARHGAP27 WW domain #2

    Plasmid

    #104283

    Purpose

    Bacterial expression of WW domain #2 from ARHGAP27

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    ARHGAP27 WW domain #2 (ARHGAP27 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHH0103 PDZK10 WW domain

    Plasmid

    #104285

    Purpose

    Bacterial expression of WW domain from PDZK10

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    PDZK10 WW domain (FRMPD4 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHH0103 KIAA0082 WW domain

    Plasmid

    #104288

    Purpose

    Bacterial expression of WW domain from KIAA0082

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    KIAA0082 WW domain (CMTR1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHH0103 DiGeorge WW domain

    Plasmid

    #104292

    Purpose

    Bacterial expression of WW domain from DiGeorge

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    DiGeorge WW domain (DGCR8 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHH0103 FNBP4 WW domain #1

    Plasmid

    #104263

    Purpose

    Bacterial expression of WW domain #1 from FNBP4

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    FNBP4 WW domain #1 (FNBP4 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHH0103 FNBP4 WW domain #2

    Plasmid

    #104264

    Purpose

    Bacterial expression of WW domain #2 from FNBP4

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    FNBP4 WW domain #2 (FNBP4 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHH0103 DRP2 WW domain

    Plasmid

    #104270

    Purpose

    Bacterial expression of WW domain from DRP2

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    DRP2 WW domain (DRP2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge