We narrowed to 16,253 results for: form

1. MBP-TEVcs(ENLYFQ/G)-His6-uTEV2Δ(220-242)-R5

   Plasmid

   #135456

   Purpose

   Bacterial expression of uTEV2Δ protease

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
   

   Tags

   MBP, His6

   Expression

   Bacterial

   Mutation

   S219V mutation improves stability, T30A/S153N mut…

   Promoter

   tac promoter

   Available Since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMSCVhyg-3xT7-nSREBP1a

   Plasmid

   #171030

   Purpose

   Expresses 3xT7-tagged nuclear form of SREBP1a in mammalian cells

   Depositor

   Kai Ge

   Article

   Jang et al Genes Dev. 2021 Apr 22. pii: gad.347583.

   Insert

   nSREBP1a (Srebf1 Mouse)
   

   Use

   Retroviral

   Tags

   3xT7

   Expression

   Mammalian

   Mutation

   amino acids 1- 449

   Promoter

   5' LTR

   Available Since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MBP-TEVcs(ENLYFQ/G)-His6-uTEV1Δ(220-242)-R5

   Plasmid

   #135455

   Purpose

   Bacterial expression of uTEV1Δ protease

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   MBP-TEVcs(ENLYFQ/G)-His6-TEV1Δ(220-242)-R5
   

   Tags

   MBP, His6

   Expression

   Bacterial

   Mutation

   S219V mutation improves stability, S153N mutation…

   Promoter

   tac promoter

   Available Since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-myc NES-mCE NLS

   Plasmid

   #82466

   Purpose

   Expresses cytoplasmic restricted form of myc tagged mRNA capping enzyme

   Depositor

   Daniel Schoenberg

   Article

   Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

   Insert

   NES mRNA capping enzyme (Rngtt HIV, Mouse)
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GFP-Rab7Q67L

   Plasmid

   #169038

   Purpose

   Express human constitutively active form of Rab7a(Q67L) in mammalian cells

   Depositor

   Gia Voeltz

   Article

   Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

   Insert

   Rab7a (RAB7A Human)
   

   Tags

   acGFP1

   Expression

   Mammalian

   Mutation

   Q67L

   Promoter

   CMV

   Available Since

   Jan. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA-Flag-Kdm6b(1025-End)-pA

   Plasmid

   #100278

   Purpose

   In vitro transcription template (T7 promoter) for Kdm6b catalytic domain active form.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nature. 2017 Jul 19. doi: 10.1038/nature

   Insert

   Kdm6b (Kdm6b Mouse)
   

   Tags

   Flag epitope tag

   Expression

   Mammalian

   Mutation

   catalytic domain (1025aa-End)

   Promoter

   T7

   Available Since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMT-Hrp48-ADARcd-E488Q-V5

   Plasmid

   #139685

   Purpose

   Expresses the hyperactive form of the catalytic domain of the ADAR protein fused to HRP-48 for use in "TRIBE" assays

   Depositor

   Michael Rosbash

   Article

   Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

   Insert

   Hrp48, ADAR (Adar Fly)
   

   Tags

   V5

   Expression

   Bacterial and Insect

   Available Since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MSCV_JAK3_IRES_BFP

   Plasmid

   #206291

   Purpose

   Expresses the wild type form of human JAK3.

   Depositor

   Charles de Bock

   Article

   Omari et al Heliyon. 2023 Nov 8;9(11):e22085. doi: 1

   Insert

   JAK3 (JAK3 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SAE1

   Plasmid

   #175241

   Purpose

   Bacterial expression and purification, combined with SAE2 to form the E1 enzyme heterodimer

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   SAE1 (SAE1 Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SAE2-mGarnet

    Plasmid

    #175240

    Purpose

    Bacterial expression and purification, combined with SAE1 to form the E1 enzyme heterodimer

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    UBA2 (UBA2 Human)
    

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5

    Plasmid

    #135454

    Purpose

    Bacterial expression of TEVΔ protease

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
    

    Tags

    MBP, His6

    Expression

    Bacterial

    Mutation

    S219V mutation improves stability. Truncated form…

    Promoter

    tac promoter

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLVX-TRE3G-LbNOX-Flag

    Plasmid

    #234980

    Purpose

    Expresses LbNOX in mouse cell lines

    Depositor

    Denis Titov

    Article

    Choe et al Cell Chem Biol. 2025 Apr 17;32(4):620-63

    Insert

    LbNOX
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLVX-TRE3G-mitoLbNOX-Flag

    Plasmid

    #234981

    Purpose

    Expresses mitochondrial LbNOX in mouse cell lines

    Depositor

    Denis Titov

    Article

    Choe et al Cell Chem Biol. 2025 Apr 17;32(4):620-63

    Insert

    mito-LbNOX
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-ShhN-Ren

    Plasmid

    #37677

    Depositor

    Philip Beachy

    Article

    Ma et al Cell. 2002 Oct 4;111(1):63-75.

    Insert

    shh (Shh Mouse)
    

    Tags

    renilla luciferase

    Expression

    Mammalian

    Mutation

    soluble form (see comments)

    Promoter

    CMV

    Available Since

    Nov. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1-Pert Heavy chain-SpyTag003

    Plasmid

    #216309

    Purpose

    Expresses anti-HER2 Pert Heavy chain-SpyTag003 in mammalian cells. To be paired with Pert light chain to form the anti-HER2 Pert Fab (related to Pertuzumab)

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    H2-18 Heavy chain-SpyTag003
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6 tag, Signal peptide, and SpyTag003

    Expression

    Mammalian

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1-Tras heavy chain-GSG-SpyTag003

    Plasmid

    #216292

    Purpose

    Expresses anti-HER2 Tras Heavy chain-GSG-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    Tras heavy chain-GSG-SpyTag003
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6 tag, Signal peptide, and SpyTag003

    Expression

    Mammalian

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYPQ262m3

    Plasmid

    #149556

    Purpose

    Gateway entry clone for CRISPR-iSpyMacCas9 ABE7.10 A-G base editing at NAAR PAMs

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

    Insert

    ecTadAwt-ecTadAmut-ziSpyMacCas9(D10A)
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A, R221K, N394K, Protospacer interacting domai…

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV-SFFV-RAB10 Q68L-WPRE-UbC-Emerald

    Plasmid

    #203803

    Purpose

    Lentiviral vector plasmid expressing human RAB10 mutation Q68L (constitutively active mutant) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Inserts

    RAB10 (RAB10 Human)
    Emerald
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Q68L

    Available Since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV-SFFV-RAB10 T23N-WPRE-UbC-Emerald

    Plasmid

    #203802

    Purpose

    Lentiviral vector plasmid expressing human RAB10 mutation T23N (dominant negative mutant) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Inserts

    RAB10 (RAB10 Human)
    Emerald
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    T23N

    Available Since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PX544 SpCas9(del1098-1368)

    Plasmid

    #58889

    Purpose

    Truncated form of SpCas9 without C' domain

    Depositor

    Feng Zhang

    Article

    Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    del amino acids 1098-1368

    Available Since

    Sept. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge