We narrowed to 5,839 results for: pCas

1. pCAGGS-mTagBFP2-T2A-iCre (v2)

   Plasmid

   #125822

   Purpose

   Monomeric TagBFP2 and iCre recombinase (version 2)

   Depositor

   Mario Capecchi

   Insert

   mTagBFP2-T2A-iCre
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Available Since

   June 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV pCAG-mRuby3-WPRE

   Plasmid

   #107744

   Purpose

   Can be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.

   Depositor

   Rylan Larsen

   Article

   Zhuang et al Cell Rep. 2021 Oct 12;37(2):109826. doi:

   Insert

   mRuby3
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDY0750 pCAG-NLS-BceINT

   Plasmid

   #179112

   Purpose

   BceINT expression vector

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   pCAG-NLS-BceINT
   

   Use

   CRISPR

   Available Since

   Feb. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pScalps eSpCas9_Zsgreen gRNA scaffold

   Plasmid

   #188449

   Purpose

   Expression of eSpCas9 tagged with Zsgreen

   Depositor

   Ajit Chande

   Article

   Mishra et al Mol Ther Nucleic Acids. 2022 Mar 8;28:20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Promoter

   EF1-alpha

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330GFP-hU6-gRNA-hSpCas9

   Plasmid

   #128385

   Purpose

   GFP positive gRNA/hSpCas9 construct

   Depositor

   Budd A. Tucker

   Article

   Giacalone et al Curr Protoc Stem Cell Biol. 2018 Feb 28;

   Insert

   GFP, gRNA and hSpCas9
   

   Use

   CRISPR

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-mEGFP-CaMKIIa (T286D)

   Plasmid

   #127391

   Purpose

   Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D)

   Depositor

   Ryohei Yasuda

   Article

   Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

   Insert

   calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   changed Threonine 286 to Aspartic acid

   Promoter

   pCAG

   Available Since

   Jan. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS-SspB-mScarlet-I-MYPT169

   Plasmid

   #178522

   Purpose

   Expresses SspB-mScarlet-I-MYPT (1-169 aa) in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

   Insert

   SspB-mScarlet-I-MYPT169
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHLS-EF1a-FRB-SpCas9-A

   Plasmid

   #138477

   Purpose

   Expresses FRB fused SpCas9 for NanoMEDIC packaging.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   SpCas9, human codon optimized
   

   Use

   CRISPR

   Tags

   3x HA tag and FRB

   Expression

   Mammalian

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9(BB)-2A-tomato SOX2-sgRNA

   Plasmid

   #196190

   Purpose

   Editing human SOX2 locus

   Depositor

   Jürgen Knoblich

   Article

   Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

   Insert

   SOX2 (SOX2 Human)
   

   Use

   CRISPR

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-hGPR56-IRES-GFP

    Plasmid

    #52297

    Purpose

    Expresses human GPR56 and GFP in mammalian cells.

    Depositor

    Christopher A Walsh

    Article

    Bae et al Science. 2014 Feb 14;343(6172):764-8. do

    Insert

    Human GPR56 coding sequence (ADGRG1 Human)
    

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. SpCas9 nickase for Split-PE (pJUL2497)

    Plasmid

    #190104

    Purpose

    Expresses nickase SpCas9(H840A) for Split-PE use

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    nSpCas9
    

    Tags

    XTEN-bpNLS and bpNLS

    Expression

    Mammalian

    Mutation

    H840A

    Promoter

    CMV

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS Donor mClover-LMNA

    Plasmid

    #98970

    Purpose

    Donor for endogenous N-ter tagging of hLMNA with mClover. Cas9 Homologous Recombination Reporter

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA5'UTR-mClover-LMNA5'exon1 (LMNA Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-HA-rat Nrxn3beta AS4(-)

    Plasmid

    #58269

    Purpose

    Expression plasmid for HA-tagged rat Neurexin 3beta, lacking the insert at alternatively spliced segment 4 (AS4-)

    Depositor

    Peter Scheiffele

    Article

    Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

    Insert

    Nrxn3beta AS4(-) (Nrxn3 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-AsCas12a-Plus-mCherry

    Plasmid

    #191231

    Purpose

    To express the AsCas12a nuclease variant "AsCas12a-Plus" that enhanced the editing activity and specificity

    Depositor

    Zhili Rong

    Article

    Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

    Insert

    AsCas12a-Plus-P2A-mCherry
    

    Use

    CRISPR

    Mutation

    E174R, R951K, R955A

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-Kir2.1Mut-T2A-tdTomato

    Plasmid

    #60644

    Purpose

    Expresses Kir2.1Mut-T2A-tdTomato

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Insert

    Kcnj2 (Kcnj2 Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    GYG to AAA (aa144-146)

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. SpCas9-NRCH-YE1-BE4max

    Plasmid

    #198549

    Purpose

    Expresses human codon-optimized SpCas9-NRCH-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NRCH-YE1-BE4max-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH-YE1-BE4max
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGGS SARS-CoV-2 BA.1 Spike

    Plasmid

    #185452

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 BA.1 Spike

    Depositor

    Marceline Côté

    Article

    Chatterjee et al Cell Rep. 2022 Mar 1;38(9):110429. doi:

    Insert

    SARS-CoV-2 Spike BA.1 (S )
    

    Expression

    Mammalian

    Mutation

    Contains the following mutations: A67V, Δ69-70, T…

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available Since

    June 8, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. eSpCas9(1.1)-T2A-Puro

    Plasmid

    #101039

    Purpose

    Enhanced SpCas9(1.1) with puromycin resistance gene via T2A linker.

    Depositor

    Andrea Németh

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3xFLAG and T2A-Puro

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Nov. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-3xFLAG-BioID2-MCS

    Plasmid

    #186811

    Purpose

    CAG promoter-driven expression of BioID2-fused protein (N terminus)

    Depositor

    Masahito Ikawa

    Article

    Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

    Type

    Empty backbone

    Tags

    3xFLAG-BioID2

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-MCP-SRSF1-V5-mCherry

    Plasmid

    #235088

    Purpose

    SRSF1 mCherry fusion protein with MCP domain

    Depositor

    Mitchell Guttman

    Article

    Bhat et al Nature. 2024 May;629(8014):1165-1173. do

    Insert

    pCAG-MCP-SRSF1-V5-mCherry (SRSF1 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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