We narrowed to 4,698 results for: pcr

1. L0-I15 tUBQ1 GG4-GG6

   Plasmid

   #195897

   Purpose

   Level 0 tUBQ1 terminator with GG4 GG6 golden gate linkers

   Depositor

   Jennifer Nemhauser

   Article

   Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

   Insert

   tUBQ1 (UBQ1 Mustard Weed)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. hp-50-0-50%GC-Left-half-120bp

   Plasmid

   #169206

   Purpose

   Force-activating hairpin for 3'-to-5' helicase

   Depositor

   Tom Perkins

   Article

   Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

   Insert

   50-0-50%GC-Left-half-120bp-hairpin
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. hp-50-0-50%GC-Right-half-120bp

   Plasmid

   #169207

   Purpose

   50-0-50%GC-Right-half-120bp-hairpin

   Depositor

   Tom Perkins

   Article

   Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

   Insert

   50-0-50%GC-Right-half-120bp-hairpin
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221-kcnj12a

   Plasmid

   #173141

   Purpose

   This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

   Depositor

   GuangJun Zhang

   Article

   Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

   Insert

   kcnj12a (kcnj12a Zebrafish)
   

   Use

   Pcr cloning vector

   Tags

   Expression

   Mutation

   Promoter

   No promoter at 5 end; T7 promoter at 3 end.

   Available since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR-D-kcnj16

   Plasmid

   #173146

   Purpose

   This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

   Depositor

   GuangJun Zhang

   Article

   Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

   Insert

   kcnj16a (kcnj16 Zebrafish)
   

   Use

   Pcr cloning vector

   Tags

   Expression

   Mutation

   Stop codon removed

   Promoter

   No promoter at 5 end; T7 promoter at 3 end.

   Available since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKI-cjOCT4-Cyan-pac

   Plasmid

   #160871

   Purpose

   Marmoset Targeting

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Stem Cell Res. 2021 Mar 24;53:102308. do

   Insert

   mCerulean3
   

   Use

   Marmoset targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR-D-TOPO-kcnt2a

   Plasmid

   #164963

   Purpose

   Zebrafish kcnt2a gene CDS

   Depositor

   GuangJun Zhang

   Article

   Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

   Insert

   kcnt2a (kcnt2 Zebrafish)
   

   Use

   Pcr cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221-kcnma1a

   Plasmid

   #164951

   Purpose

   Zebrafish kcnma1a gene CDS

   Depositor

   GuangJun Zhang

   Article

   Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

   Insert

   kcnma1a (kcnma1a Zebrafish)
   

   Use

   Pcr cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGuide-P1-O1a (EL702)

   Plasmid

   #140040

   Purpose

   CRISPR DuMPLING negative control plasmid with probe/barcode P1 and negative control lacO1array spacer in the sgRNA. Also template for library PCRs.

   Depositor

   Johan Elf

   Article

   Camsund et al Nat Methods. 2020 Jan;17(1):86-92. doi:

   Insert

   barcode P1 and sgRNA lacO1 array
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBP-lacZ

    Plasmid

    #72948

    Purpose

    Entry vector for Bioparts - Oligo anneal or PCR with internal BsaI sites at 5' and 3' ends using NdeI/SphI cloning

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 0 - Bioparts
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSG5-FLAG-CaMKKalpha N-terminus on CaMKKbeta

    Plasmid

    #33329

    Depositor

    Anthony Means

    Article

    Green et al Cell Signal. 2011 Dec;23(12):2005-12. Ep

    Insert

    Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    CaMKKalpha N-terminus(1-128) was fused with CaMKK…

    Promoter

    SV40

    Available since

    April 17, 2012

    Availability

    Academic Institutions and Nonprofits only

12. -2.5Kb MMP10

    Plasmid

    #53973

    Purpose

    Luciferase reporter for mouse MMP10 2.5kb promoter

    Depositor

    Michael Chin

    Article

    Wu et al Biochem Biophys Res Commun. 2011 Dec 2;4

    Insert

    mouse MMP10 2.5Kb promoter (Mmp10 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PCR from C57BL6/J

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. Integrin beta 2 - mYFP

    Plasmid

    #8638

    Depositor

    Timothy Springer

    Article

    Kim et al Science 2003 Sep 19;301(5640):1720-5.

    Insert

    Integrin beta 2 (ITGB2 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    stop codon of beta2 was replaced with AgeI restri…

    Promoter

    Available since

    July 18, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS-mEGFP-loxP-P2A-dTKneo-pA-loxP

    Plasmid

    #139484

    Purpose

    PCR template for mEGFP gene knock-in donor vector

    Depositor

    Kazuhiro Aoki

    Article

    Komatsubara et al J Biol Chem. 2019 Apr 12;294(15):6062-60

    Insert

    mEGFP-loxP-P2A-dTKneo-pA-loxP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHyg-AID*

    Plasmid

    #99515

    Purpose

    C-terminal AID* degron cassette with HygR marker for S. cerevisiae

    Depositor

    Helle Ulrich

    Article

    Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

    Insert

    IAA17(71-114) (AXR3 Mustard Weed)
    

    Use

    Pcr template for yeast tagging cassette

    Tags

    Expression

    Mutation

    internal inversion of nucleotides 265-312 of the …

    Promoter

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKan-PRFA1-9myc-AID*(N)

    Plasmid

    #99528

    Purpose

    N-terminal 9myc-AID* degron cassette with RFA1 promoter and KanR marker for S. cerevisiae

    Depositor

    Helle Ulrich

    Article

    Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

    Insert

    IAA17(71-117) (AXR3 Mustard Weed)
    

    Use

    Pcr template for yeast tagging cassette

    Tags

    9xMyc

    Expression

    Mutation

    Promoter

    RFA1

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSJ1458 (pFA6-link-mTurquoise2-CaURA3MX)

    Plasmid

    #86424

    Purpose

    PCR tagging module with yeast codon optimized mTurquoise2

    Depositor

    Sue Jaspersen

    Article

    Burns et al Elife. 2015 Sep 15;4. doi: 10.7554/eLife

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKan-PCUP1-9myc-AID*(N)

    Plasmid

    #99527

    Purpose

    N-terminal 9myc-AID* degron cassette with CUP1 promoter and KanR marker for S. cerevisiae

    Depositor

    Helle Ulrich

    Article

    Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

    Insert

    IAA17(71-117) (AXR3 Mustard Weed)
    

    Use

    Pcr template for yeast tagging cassette

    Tags

    9xMyc

    Expression

    Mutation

    Promoter

    CUP1

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKan-PCUP1-AID*(N)-9myc

    Plasmid

    #99526

    Purpose

    N-terminal AID*-9myc degron cassette with CUP1 promoter and KanR marker for S. cerevisiae

    Depositor

    Helle Ulrich

    Article

    Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

    Insert

    IAA17(71-116) (AXR3 Mustard Weed)
    

    Use

    Pcr template for yeast tagging cassette

    Tags

    9xMyc

    Expression

    Mutation

    Promoter

    CUP1

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAGGS-Halotag-loxP-P2A-dTKneo-pA-loxP

    Plasmid

    #139485

    Purpose

    PCR template for Halo-Tag gene knock-in donor vector

    Depositor

    Kazuhiro Aoki

    Article

    Komatsubara et al J Biol Chem. 2019 Apr 12;294(15):6062-60

    Insert

    HaloTag-loxP-P2A-dTKneo-pA-loxP
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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