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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,240 results for: spl

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Showing: 1601 - 1620 of 4240 results

  2. pM355

    Plasmid
    #173175
    Purpose
    (Empty Backbone) Expresses gRNA-12xMBS from hU6 promoter
    Depositor
    Hajin Kim
    Publication
    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA-12xMBS backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    Sept. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    173175-plasmid-map-sequence-id-341548

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  3. pSLQ8518 PB-PGK-N term (1-406) dLbCpf1-EE12RR345L-pA

    Plasmid
    #140221
    Purpose
    Constitutive PGK driven expression of N terminal dCpf1 (Cas12a) 406-split half with leucine zipper
    Depositor
    Stanley Qi
    Publication
    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCpf1 (Cas12a) N terminal half (406) with zipper
    Expression
    Mammalian
    Promoter
    PGK
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140221-plasmid-map-sequence-id-291991

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  6. mCherry-hPMCA2wb

    Plasmid
    #47752
    Purpose
    mammalian expression of mCherry tagged hPMCA2, wb splice variant
    Depositor
    Emanuel Strehler
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PMCA2w/b (ATP2B2 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    wb splice variant
    Promoter
    CMV
    Available since
    Nov. 23, 2018
    Availability
    Academic Institutions and Nonprofits only

  7. mCherry-hPMCA2zb

    Plasmid
    #47750
    Purpose
    mammalian expression of mCherry tagged hPMCA2, zb splice variant
    Depositor
    Emanuel Strehler
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PMCA2z/b (ATP2B2 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    zb splice variant
    Promoter
    CMV
    Available since
    Nov. 23, 2018
    Availability
    Academic Institutions and Nonprofits only

  8. pU6_B2M_sgRNA(PP7)

    Plasmid
    #232438
    Purpose
    sgRNA to facilitate a splice donor site disruption via adenine base editing in the B2M locus, contains a PP7 aptamer in the scaffold tetraloop
    Depositor
    Gil Westmeyer
    Publication
    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PP7-tagged gRNA for B2M disruption via splice donor consensus disruption via ABE8e or Cas9 driven by human U6 promoter
    Use
    CRISPR
    Promoter
    U6
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    232438-plasmid-map-sequence-id-462755

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  15. pcDNA3-intein/Src

    Plasmid
    #71406
    Purpose
    Expresses Src inserted with a Npu DnaE intein (F1 construct)
    Depositor
    Huiwang Ai
    Publication
    Ren et al J Am Chem Soc. 2015 Feb 18;137(6):2155-8
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Src active kinase domain inserted with Npu DnaE intein
    Expression
    Mammalian
    Promoter
    pCMV
    Available since
    Jan. 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  20. FKBP-CD28TMD-NTEVp(H75S)

    Plasmid
    #137828
    Purpose
    MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75S mutant TEV protease
    Depositor
    Joshua Leonard
    Publication
    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FKBP-CD28TMD-NTEVp(H75S)
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    Mutated Histidine 75 to Serine in NTEVp fragment
    Promoter
    CMV
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    137828-plasmid-map-sequence-id-311924

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Showing: 1601 - 1620 of 4240 results