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B*57:03:01

Plasmid

#203260

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*57:03:01 (HLA-B Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pPBJ pFOS-KTR-Gal4-PEST-tub3’ pCMV-TagBFP

Plasmid

#176531

Purpose

PiggyBAC vector expressing a FOS promoter driven "KGV" synthetic transcription factor and a CMV promoter driven BFP marker.

Depositor

Jared Toettcher

Article

Ravindran et al Cell Syst. 2022 Feb 16;13(2):131-142.e13

Inserts

KGV transcription factor
TagBFP

Use

Piggybac transposase integration vector

Tags

ErkKTR, Gal4 DNA binding domain, PEST destabiliza…

Expression

Mammalian

Available Since

March 7, 2024

Availability

Academic Institutions and Nonprofits only

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SPHK2 gRNA (BRDN0001148465)

Plasmid

#77093

Purpose

3rd generation lentiviral gRNA plasmid targeting human SPHK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SPHK2 (SPHK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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eTd-CBE

Plasmid

#196601

Purpose

Mammalian expression of eTd-CBE

Depositor

Dali Li

Article

Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

Insert

bpNLS-TadA-8e (N46L)-nSpCas9-bpNLS-P2A-UGI-bpNLS

Tags

6*His

Expression

Mammalian

Available Since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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pSL5069 (pDonor(AAVS1),CRISPR_Pse)

Plasmid

#200904

Purpose

Encodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a splice acceptor-T2A-PuroR-T2A-eGFP cargo, and a CRISPR RNA under a U6 promoter. Total transposon size = 2,024 bp.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

Mini Tn7016 (AAVS1 NGS), PseCRISPR (tSL425)

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pv9_CBX1_2xChromo-13XL-BASU

Plasmid

#179412

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1.2xChromo-13XL-BASU

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX1.2xChromo-13XL-BASU

Expression

Mammalian

Promoter

CAGGS

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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Polymerase expression - pCMV-Phi29 (LM2708)

Plasmid

#208965

Purpose

Unfused Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

Phi29-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

Phi29(-exo;D169A)

Promoter

CMV and T7

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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B-HiFi SpCas9

Plasmid

#207362

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HiFi SpCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

R691A, amino acids 1005-1013 replaced with two gl…

Promoter

CBh

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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PRKAG3 gRNA (BRDN0001145578)

Plasmid

#77295

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKAG3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKAG3 (PRKAG3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKAG3 gRNA (BRDN0001147132)

Plasmid

#77296

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKAG3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKAG3 (PRKAG3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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