We narrowed to 1,804 results for: FAST

1. RcSxph Y795A

   Plasmid

   #194043

   Purpose

   Protein expression and characterization

   Depositor

   Dan Minor

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

   Insert

   Rana catesbeiana Saxiphilin
   

   Tags

   His10 and mEGFP,

   Expression

   Insect

   Mutation

   Y795A

   Promoter

   Polyhedrin

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. RcSxph Y558A/I782A

   Plasmid

   #194044

   Purpose

   Protein expression and characterization

   Depositor

   Dan Minor

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

   Insert

   Rana catesbeiana Saxiphilin
   

   Tags

   His10 and mEGFP,

   Expression

   Insect

   Mutation

   Y558A/I782A

   Promoter

   Polyhedrin

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. FAK Fus

   Plasmid

   #186145

   Purpose

   For expression of recombinant FAK using baculovirus in SF9 cells

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   PTK2 (PTK2 Mouse)
   

   Tags

   His6

   Expression

   Insect

   Mutation

   C-terminal linker swapped with FUS IDR

   Promoter

   polyhedrin promoter

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lck

   Plasmid

   #186130

   Purpose

   For expression of recombinant Lck in SF9 cells.

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   Lck (Lck Mouse)
   

   Tags

   His6

   Expression

   Insect

   Mutation

   A1687T

   Promoter

   polyhedrin promoter

   Available Since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. FAK Pyk2

   Plasmid

   #186146

   Purpose

   For expresseion of recombinant FAK using baccolovirus in SF9 cells

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   PTK2 (PTK2 Mouse)
   

   Tags

   His6

   Expression

   Insect

   Mutation

   C-terminal linker swapped with Pyk2 linker

   Promoter

   polyhedrin promoter

   Available Since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Mdn1-deltaC

   Plasmid

   #171603

   Purpose

   Expresses S. pombe Mdn1 a.a. 1-3911 in insect cells.

   Depositor

   Tarun Kapoor

   Article

   Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

   Insert

   Mdn1
   

   Tags

   His6 tag

   Expression

   Insect

   Mutation

   C-terminal deletion; a.a. 1-3911 only

   Available Since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Ai148 (TIT2L-GC6f-ICL-tTA2) targeting vector

   Plasmid

   #114428

   Purpose

   Target a Cre-dependent GCaMP6f expression and a tTA2 cassette into the mouse TIGRE locus

   Depositor

   Hongkui Zeng

   Article

   Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

   Insert

   GCaMP6f
   

   Use

   Cre/Lox and Mouse Targeting

   Promoter

   TRE2, CAG

   Available Since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CaMKIIa-hChR2(E123T/T159C)-EYFP

   Plasmid

   #35511

   Purpose

   AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Insert

   hChR2
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E123T and T159C

   Promoter

   CaMKIIa

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFBD-Akt1(D323A/D325A)

   Plasmid

   #86592

   Purpose

   Expresses human full-length Akt1(D323A/D325A) mutant

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Tags

   10xHis

   Expression

   Insect

   Mutation

   D323A/D325A transforming mutations

   Available Since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pECNUS4

    Plasmid

    #184887

    Purpose

    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

    Depositor

    Eunyoung Chae

    Article

    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    RPS5A

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSR657

    Plasmid

    #65214

    Purpose

    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cells

    Depositor

    Robert Kotin

    Article

    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

    Inserts

    modified AAV-2 rep78
    modified AAV-2 cap
    

    Use

    AAV

    Expression

    Insect

    Promoter

    p10 and polyhedrin

    Available Since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSR646

    Plasmid

    #65215

    Purpose

    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-6 cap gene in Sf9 insect cells

    Depositor

    Robert Kotin

    Article

    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

    Inserts

    modified AAV-2 rep78
    modified AAV-6 cap
    

    Use

    AAV

    Expression

    Insect

    Promoter

    P10 and polyhedrin

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSR660

    Plasmid

    #65216

    Purpose

    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cells

    Depositor

    Robert Kotin

    Article

    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

    Inserts

    modified AAV-2 rep78
    modified AAV-8 cap
    

    Use

    AAV

    Expression

    Insect

    Promoter

    P10 and polyhedrin

    Available Since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. FLAG-LoxP-PURO-LoxP-HaloTERT WT HR Donor

    Plasmid

    #86843

    Purpose

    HR Donor for N-terminal FLAG-HaloTag-TERT fusion in human cells. Includes Puro selection marker flanked by LoxP sites.

    Depositor

    Thomas Cech

    Article

    Schmidt et al Cell. 2016 Aug 25;166(5):1188-1197.e9. d

    Insert

    FLAG-HaloTag-TERT Exon 1 and part of Exon 2 (TERT Human)
    

    Tags

    FLAG-HaloTag

    Expression

    Mammalian

    Promoter

    Endogenous TERT promoter

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSR651

    Plasmid

    #65213

    Purpose

    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-1 cap gene in Sf9 insect cells

    Depositor

    Robert Kotin

    Article

    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

    Inserts

    modified AAV-2 rep78
    modified AAV-1 cap
    

    Use

    AAV

    Expression

    Insect

    Promoter

    p10 and polyhedrin

    Available Since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. p130Cas Y15F

    Plasmid

    #186154

    Purpose

    For expressing recombinant p130Cas in insect cells.

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    BCAR1 (Bcar1 Rat)
    

    Tags

    His6 (Tev)

    Expression

    Insect

    Mutation

    15Y residues mutated to Alanine

    Promoter

    polyhedrin

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CaMKIIa-hChR2(E123A)-mCherry

    Plasmid

    #35506

    Purpose

    AAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    hChR2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E123A

    Promoter

    CaMKIIa

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFBD-Akt1(E17K)

    Plasmid

    #86563

    Purpose

    Expresses human full-length Akt1(E17K) mutant

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Tags

    10xHis

    Expression

    Insect

    Mutation

    E17K mutation in the PH domain

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFBD-Akt1(T308A/S473A)

    Plasmid

    #86578

    Purpose

    Expresses human full-length Akt1(T308A/S473A) phosphomutant

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Tags

    10xHis

    Expression

    Insect

    Mutation

    T308A/S473A phosphomutant

    Available Since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFBD-Akt1(K14A)

    Plasmid

    #86562

    Purpose

    Expresses human full-length Akt1(K14A) mutant

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Tags

    10xHis

    Expression

    Insect

    Mutation

    K14A mutation in the PH domain

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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