We narrowed to 2,328 results for: dap

1. MSCV-NSD3-short

   Plasmid

   #72552

   Purpose

   expresses 3*FLAG tagged human NSD3-short

   Depositor

   Christopher Vakoc

   Article

   Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

   Insert

   NSD3-short (NSD3 Human)
   

   Use

   Retroviral

   Tags

   3*FLAG

   Expression

   Mammalian

   Mutation

   none

   Promoter

   LTR

   Available since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCh-BICD2*-strep

   Plasmid

   #120168

   Purpose

   Expresses a chimera of mCherry (fluorescent tag), a BICD2-based adaptor and streptavidin

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   BICD cargo adaptor 2 (Bicd2 Mouse)
   

   Use

   Tags

   Streptavidin and mCherry

   Expression

   Mammalian

   Mutation

   Truncated BICD2: 15-595 aa

   Promoter

   CMV

   Available since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTIGER_mNeonGreen::3xFLAG::dCrk

   Plasmid

   #131137

   Purpose

   UASp construct for over-expression of Drosophila Crk with N-terminal monomeric NeonGreen and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   Crk (Crk Fly)
   

   Use

   Tags

   3XFLAG and mNeonGreen

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-C-moxGFP-Puro-H3C2

   Plasmid

   #207783

   Purpose

   Donor template for moxGFP-2A-Puro insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H3C2 Homology Arms flanking a moxGFP-Puro Cassette (H3C2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pTIGER_tdTomato::3xFLAG::dCrk

   Plasmid

   #131138

   Purpose

   UASp construct for over-expression of Drosophila Crk with N-terminal tandem Tomato and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   Crk (Crk Fly)
   

   Use

   Tags

   3XFLAG and tdTomato

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-N-Blast-sTagRFP-TUBA1B

   Plasmid

   #207768

   Purpose

   Donor template for Blast-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TUBA1B Homology Arms flanking a Blast-sTagRFP Cassette (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pMKO.1-shCcnb1

   Plasmid

   #162793

   Purpose

   Ccnb1 shRNA in pMKO.1 retroviral vector

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Ccnb1 shRNA (Ccnb1 Mouse)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-APPL1-R146A/K152A/R154A

   Plasmid

   #59767

   Purpose

   Expresses APPL1 Endosomal Localization Mutant

   Depositor

   Donna Webb

   Article

   Broussard et al Mol Biol Cell. 2012 Apr;23(8):1486-99. d

   Insert

   APPL1 (APPL1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   R146A/K152A/R154A

   Promoter

   CMV

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pCDF-casBCDE(g8)

   Plasmid

   #89727

   Purpose

   Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing 33-nt spacer (referred to as 'wt') matching a protospacer in the M13 phage genome.

   Depositor

   Konstantin Kuznedelov, Konstantin Severinov

   Article

   Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

   Insert

   CRISPR array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. TRBV16

    Plasmid

    #123451

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRBV16 (TRBV16 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. integrin alpha9 W999A pcDNA1 neo

    Plasmid

    #13589

    Depositor

    Dean Sheppard

    Article

    Young et al Mol Biol Cell. 2001 Oct . 12(10):3214-25

    Insert

    integrin alpha 9 W999A (ITGA9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W999A

    Promoter

    Available since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEMHE-MmCENPT-ASD2HFD-GFP

    Plasmid

    #237430

    Purpose

    Expresses Mus musculus CENP-T with ancestral HFD (mouse:rat); tagged with GFP at C-term; made for in vitro transcription (T7 promoter)

    Depositor

    Michael Lampson

    Article

    Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

    Insert

    Mus musculus CENP-T with ancestral HFD (Cenpt Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    HFD (residues 413-503) swapped from mouse:rat anc…

    Promoter

    T7

    Available since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEMHE-MmCENPT-RpHFD-GFP

    Plasmid

    #237428

    Purpose

    Expresses Mus musculus CENP-T with Rhabdomys pumilio HFD; tagged with GFP at C-term; made for in vitro transcription (T7 promoter)

    Depositor

    Michael Lampson

    Article

    Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

    Insert

    Mus musculus CENP-T with Rhabdomys pumilio HFD (Cenpt Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    HFD (residues 413-503) swapped from Rhabdomys pum…

    Promoter

    T7

    Available since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEMHE-MmCENPT-McHFD-GFP

    Plasmid

    #237437

    Purpose

    Expresses Mus musculus CENP-T with Mus caroli HFD; tagged with GFP at C-term; made for in vitro transcription (T7 promoter)

    Depositor

    Michael Lampson

    Article

    Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

    Insert

    Mus musculus CENP-T with Mus caroli HFD (Cenpt Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    HFD (residues 413-503) swapped from Mus caroli

    Promoter

    T7

    Available since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEMHE-MmCENPT-RnHFD-GFP

    Plasmid

    #237429

    Purpose

    Expresses Mus musculus CENP-T with Rattus norvegicus HFD; tagged with GFP at C-term; made for in vitro transcription (T7 promoter)

    Depositor

    Michael Lampson

    Article

    Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

    Insert

    Mus musculus CENP-T with Rattus norvegicus HFD (Cenpt Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    HFD (residues 413-503) swapped from Rattus norveg…

    Promoter

    T7

    Available since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEMHE-MmCENPT-MsHFD-GFP

    Plasmid

    #237427

    Purpose

    Expresses Mus musculus CENP-T with Mus spretus HFD; tagged with GFP at C-term; made for in vitro transcription (T7 promoter)

    Depositor

    Michael Lampson

    Article

    Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

    Insert

    Mus musculus CENP-T with Mus spretus HFD (Cenpt Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    HFD (residues 413-503) swapped from Mus spretus

    Promoter

    T7

    Available since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Str-KDEL_SBP-EGFP-LAP

    Plasmid

    #222322

    Purpose

    Synchronize the trafficking of LAP from the ER.

    Depositor

    Franck Perez

    Article

    Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

    Insert

    Streptavidin-KDEL and LAP fused to SBP-EGFP (ACP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. eft-3p:act-5(ptc):tbb2 3' UTR

    Plasmid

    #202331

    Purpose

    The ubiquitous eft-3 promoter drives overexpression of the mutant act-5(ptc) gene including introns.

    Depositor

    Didier Stainier

    Article

    Welker et al PLoS Genet. 2023 Jun 29;19(6):e1010806.

    Inserts

    eft-3 promoter
    act-5(ptc)
    tbb-2 3' UTR
    

    Use

    Tags

    base 238 C -> A mutation causing a PTC at codo…

    Expression

    Worm

    Mutation

    Promoter

    This is a promoter sequence for C. elegans eft-3,…

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. qTAG-C-Solo-mScarlet-MAPRE1

    Plasmid

    #227323

    Purpose

    Donor template for mScarlet insertion into the C-terminus of the MAPRE1 locus. For growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 (Addgene #207793)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    MAPRE1 Homology Arms flanking a mScarlet Tag (MAPRE1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. qTAG-C-Solo-mStayGold-EZR

    Plasmid

    #227294

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the EZR locus. For membrane visualization. To be co-transfected with sgRNA plasmid px330-EZR (Addgene #227293)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    EZR Homology Arms flanking a mStayGold Tag (EZR Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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