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pCHSU70.2-2.2

Plasmid

#110683

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4

Expression

Yeast

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pEAHISPSVORF126

Plasmid

#97021

Purpose

Expressing Pyrobaculum spherical virus orf126 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

PSV orf 126

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPSVORF239

Plasmid

#97025

Purpose

Expressing Pyrobaculum spherical virus orf239 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

PSV orf 239

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPSVORF165A

Plasmid

#97024

Purpose

Expressing Pyrobaculum spherical virus orf165a protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

PSV orf 165a

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPSVORF137

Plasmid

#97023

Purpose

Expressing Pyrobaculum spherical virus orf137 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

PSV orf 137

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPSVORF131

Plasmid

#97022

Purpose

Expressing Pyrobaculum spherical virus orf131 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

PSV orf 131

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pnYC-CvH-CteIF4E_35-250-del183-202_AF

Plasmid

#148721

Purpose

Bacterial Expression of CteIF4E_35-250-del183-202

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

CteIF4E_35-250-del183-202

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-CvH-CteIF4E_40-250-del183-202_AF

Plasmid

#148723

Purpose

Bacterial Expression of CteIF4E_40-250-del183-202

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

CteIF4E_40-250-del183-202

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpH-CteIF4E_35-250-del183-202_AF

Plasmid

#148725

Purpose

Bacterial Expression of CteIF4E_35-250-del183-202

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

CteIF4E_35-250-del183-202

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLJM1-TSC22D2-Nterm-C-FLAG

Plasmid

#222683

Purpose

overexpressing N-terminus truncation of TSC22D2 with C-terminal FLAG

Depositor

Jason Moffat

Article

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

TSC22D2 (TSC22D2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

TSC22D2 N-terminus truncation

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-SREBP-1 (A317-318)

Plasmid

#204193

Purpose

Mammalian expression of SREBP-1 (isoform 1)

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-1 (SREBF1 Human)

Tags

E-tag and T7x2

Expression

Mammalian

Mutation

Gly to Ala 317, 318

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-SREBP-1 (A317-318; A341)

Plasmid

#204194

Purpose

Mammalian expression of SREBP-1 (Isoform 1)

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-1 (SREBF1 Human)

Tags

E-tag and T7x2

Expression

Mammalian

Mutation

Gly to Ala 317,318,341

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pminiTol2-4kb wnt3 promoter-Wnt3EGFP

Plasmid

#203793

Purpose

Tol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of Wnt3-EGFP for zebrafish transgenesis

Depositor

Thorsten Wohland

Article

Teh et al Development. 2015 Nov 1;142(21):3721-33.

Insert

Wnt3 (wnt3 Zebrafish)

Use

Zebrafish expression

Tags

mEGFP

Promoter

4kb Wnt3 promoter

Available Since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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GABA(A)R, Delta scFv [N151/3]

Plasmid

#206724

Purpose

Mammalian Expression of GABA(A)R, Delta scFV. Derived from hybridoma N151/3 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GABA(A)R, Delta (Rattus norvegicus) recombinant scFV (Gabrd Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pPT-PCR C2

Plasmid

#141247

Purpose

PCR cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 2

Depositor

Bogumil Karas

Article

Cochrane et al Algal Research, 2020. 50, 102014

Insert

Mitochondrial Genome of Phaeodactylum tricornutum

Use

Synthetic Biology; Diatom expression

Expression

Bacterial and Yeast

Mutation

Repeat region removed, additional minor mutations…

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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Parvalbumin scFv [L114/3]

Plasmid

#190504

Purpose

Mammalian Expression of Parvalbumin scFV. Derived from hybridoma L114/3.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Parvalbumin (Rattus norvegicus) recombinant scFV (Pvalb Mouse)

Tags

HA, 6xHis, Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Ankyrin-G scFv [N106/20]

Plasmid

#190525

Purpose

Mammalian Expression of Ankyrin-G scFV. Derived from hybridoma N106/20.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Ankyrin-G (Homo sapiens) recombinant scFV (ANK3 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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HCN4 scFv [N114/10]

Plasmid

#190526

Purpose

Mammalian Expression of HCN4 scFV. Derived from hybridoma N114/10.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

HCN4 (Homo sapiens) recombinant scFV (HCN4 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Aldh1L1 scFv [N103/31]

Plasmid

#190522

Purpose

Mammalian Expression of Aldh1L1 scFV. Derived from hybridoma N103/31.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Aldh1L1 (Rattus norvegicus) recombinant scFV (Aldh1l1 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Synaptotagmin-6 scFv [N270/47]

Plasmid

#190544

Purpose

Mammalian Expression of Synaptotagmin-6 scFV. Derived from hybridoma N270/47.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Synaptotagmin-6 (Mus musculus) recombinant scFV (Syt6 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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