We narrowed to 19,555 results for: Tec

Showing: 8311 - 8320 of 19555 results

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p2T-cmv-SpCas9-TadCBEa-BlastR

Plasmid

#193847

Purpose

Express TadCBEa in mammalian cells with BlastR selection, Tol2 integration

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadCBEa

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001145694)

Plasmid

#80191

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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PET-eSpCas9-plus-NLS-6xHis

Plasmid

#207380

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

eSpCas9-plus

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

K848A, R1060A, amino acids 1005-1013 replaced wit…

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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MLKL gRNA (BRDN0001148608)

Plasmid

#77539

Purpose

3rd generation lentiviral gRNA plasmid targeting human MLKL

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MLKL (MLKL Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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2PH-PLCd-APEX2-P2A-mKate2

Plasmid

#71284

Purpose

Mammalian expression vector driving 2PH-PLC-delta-APEX2 (Human 2xPLCd PH domain, [marks PI(4,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

2PH-PLCd-APEX2-P2A-mKate2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3A-BE3-Y130F

Plasmid

#113428

Purpose

Expresses hA3A-BE3-Y130F in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3A-BE3-Y130F (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)

Use

CRISPR

Expression

Mammalian

Mutation

hAPOBEC3A_Y130F

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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MLKL gRNA (BRDN0001146456)

Plasmid

#77540

Purpose

3rd generation lentiviral gRNA plasmid targeting human MLKL

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MLKL (MLKL Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-APOBEC1-UdgX-nCas9

Plasmid

#163555

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-APOBEC1-UdgX-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer20-Blast-CDT1-HA

Plasmid

#109335

Purpose

virus packaged, doxycyline inducible expression of Cdt1

Depositor

Jean Cook

Article

Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

Insert

Cdt1 (CDT1 Human)

Use

Lentiviral; Inducible expression

Tags

HA, His, Strep

Expression

Mammalian

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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p168-759

Plasmid

#62619

Purpose

ePathBrick pETM6 vector encoding CsF3H, AaDFR, DuLAR with Protein Scaffold GBD:SH3:PDZ (1:1:4)

Depositor

Mattheos Koffas

Article

Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

Inserts

flavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
Scaffold Protein encoding GBD:SH3:PDZ (1:1:4)

Use

Synthetic Biology

Tags

GBD, PDZ, and SH3

Expression

Bacterial

Promoter

T7

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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