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pCG_SARS-CoV1 ORF6 C-V5-tag

Plasmid

#179970

Purpose

Mammalian expression vector for SARS-CoV-1 ORF6, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

SARS-CoV-1 ORF6 (ORF6 Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 E C-V5-tag

Plasmid

#179966

Purpose

Mammalian expression vector for SARS-CoV-1 E, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

SARS-CoV-1 E (E Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 NSP16 C-V5-tag

Plasmid

#179965

Purpose

Mammalian expression vector for SARS-CoV-1 Nsp16, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

SARS-CoV-1 Nsp16 (ORF1ab Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_CoordinatorMutant#2

Plasmid

#173986

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motif #2 mutated

Use

Luciferase

Mutation

Coordinator motif mutation #2

Promoter

SV40 promoter

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-EC1.45-human_min1-opossum_min2

Plasmid

#173966

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Human minimal enhancer region 1 (min1) and opossum minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45

Use

Luciferase

Mutation

See Depositor Comments Below

Promoter

SV40 promoter

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pKHH030_sgAMBRA1#2

Plasmid

#174147

Purpose

Lentiviral vector expressing a sgRNA against the human AMBRA1 gene

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA (AMBRA1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pKHH030_sgAMBRA1#1

Plasmid

#174146

Purpose

Lentiviral vector expressing a sgRNA against the human AMBRA1 gene

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA (AMBRA1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_7XCoordinatorMutant

Plasmid

#173994

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with all 7 Coordinator motifs mutated

Use

Luciferase

Mutation

7X Coordinator motif mutation #1-2-3-4-5-6-7

Promoter

SV40 promoter

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_min1-2_4XCoordinatorMutant

Plasmid

#173962

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with 4 mutated Coordinator motifs

Use

Luciferase

Mutation

4 mutated Coordinator motifs

Promoter

SV40 promoter

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_CoordinatorMutant#1

Plasmid

#173985

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motif #1 mutated

Use

Luciferase

Mutation

Coordinator motif mutation #1

Promoter

SV40 promoter

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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