Expression of a sensor for synthetic sequence 1 (EK0208) with three MS2 sequences in the 3' UTR in mammalian cells. Compared to EK0285, also has additional restriction sites around the sensor sequence. Recommended for the basis for cloning new sensors for use with MCP-ADAR(DD). HindIII & MluI can be used to swap out marker (mCherry). (KpnI or BamHI) and (BshTI or BcuI) can be used to swap out sensor sequence (s70587). XhoI and ApaI can be used to swap out output (EGFP).

Self complementary AAV vector for high-throughput in vivo measurement of enhancer activity by massively parallel reporter assay

Encodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Crh gene which encodes corticotropin releasing factor

For use in RBP tethering screen

Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.

AAV9 based enhancer activity assay

Bacterial expression of GST-tagged p37 that is p97 binding deficient due to mutations in the SHP box

Bacterial expression of GST-tagged p37 lacking the UBX domain

An amino-terminal CFP Fragment was fused to residues 202-477 of RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.

Lentiviral vector expressing synonymous tandem PP7 coat protein fused to synonymous tandem GFP. Used to label reporter mRNAs.

AAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI with a nuclear export signal sequence

SETDB1 knockdown

AAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI

pCDNA3.1 with Kozak (GCCACC), ADGRL3 signal peptide, SNAP-tag, GS linker, and Flag-tag (EK site) ahead of ADGRL3-CTF

AGO2 knockdown

Expression of a near-infrared genetically encoded calcium indicator iGECI with a nuclear export signal sequence

AGO2 knockdown

caCas9 integrating vector into the Neut5L locus with gene drive for targeting Ade2 locus