Addgene

We narrowed to 4,042 results for: BFP

Showing: 1661 - 1680 of 4042 results

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-1

Plasmid

#128756

Purpose

Expresses PAPD4 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

PAPD4 sgRNA #1 (TENT2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-2

Plasmid

#128757

Purpose

Expresses PAPD4 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

PAPD4 sgRNA #1 (TENT2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6(BsmBI)-EF1a-BFP-BSD-Nkx2-5

Plasmid

#117137

Purpose

Expression vector encoding BFP-BSD-Nkx2-5

Depositor

Kosuke Yusa

Article

Yu et al Stem Cells. 2019 Apr 1. doi: 10.1002/ste

Insert

Nkx2-5 CDS (Nkx2-5 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

modified BFP

Promoter

human U6 promoter, human EF1a promoter

Available since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-MTS-TagBFP-P2AT2A-EGFP-NLS-P2AT2A-mCherry-PTS1

Plasmid

#87829

Purpose

High-efficient mammalian expression vector for co-expression of BFP-, EGFP- and mCherry-tagged proteins using P2AT2A. MTS, NLS and PTS1 can be replaced by proteins of interest.

Depositor

Andrea Musacchio

Article

Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

Inserts

MTS
NLS
PTS1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter, tetracycline operator

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pSLQ2814 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SpdCas9

Plasmid

#84247

Purpose

Expresses direct fusion VPR-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

VPR-tagBFP-Sp dCas9
Puro

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), HA tag, VPR, and tagBFP

Expression

Mammalian

Mutation

Promoter

CAG and PGK

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a BFP

Plasmid

#31481

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

Traffic Light Reporter 1.1 (Sce target) EF BFP

Use

Lentiviral

Tags

Expression

Mutation

mCherry contains M9S and M16L to reduce backgroun…

Promoter

Available since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP

Plasmid

#86457

Purpose

Mouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sites

Depositor

Ralf Kuehn

Article

Chu et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

BFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Pgk

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-SpdCas9

Plasmid

#84245

Purpose

Expresses direct fusion KRAB-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

KRAB-tagBFP-Sp dCas9
Puro

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), HA tag, KRAB, and tagBFP

Expression

Mammalian

Mutation

Promoter

CAG and PGK

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SadCas9

Plasmid

#84246

Purpose

Expresses direct fusion VPR-Sa dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

VPR-tagBFP-Sa dCas9
Puro

Use

CRISPR and Synthetic Biology; Piggybac

Tags

Nucleoplasmin NLS, SV40 NLS, VPR, and tagBFP

Expression

Mammalian

Mutation

Promoter

CAG and PGK

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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uORF1-Mreinit- 36xMashTag-BFP-3xStop-24xPP7 (uORF-MashTag reporter)

Plasmid

#128609

Purpose

Expresses uORF1 -36xMashTag with MoonTag as readout for reinitiation, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.

Depositor

Marvin Tanenbaum

Article

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Insert

uORF1-36xMashTag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Pcmv

Available since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pHBS1399 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G368W+G385W]

Plasmid

#118813

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

G368W and W385G in CTD of full-length TDP43

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-SFFV-Zim3-dCas9-P2A-BFP

Plasmid

#188767

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Jonathan Weissman, Marco Jost

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Expression

Mutation

Promoter

SFFV

Available since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCMVd1-NLS-GAL4-iLight2-VP16-T2A-mTagBFP2

Plasmid

#219548

Purpose

NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

Insert

NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2

Use

Tags

Expression

Mammalian

Mutation

The (SAGG)x4 linker between GAL4(1-65) and the PC…

Promoter

CMVd1

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-SEMPER_ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

Plasmid

#221075

Purpose

SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

Depositor

Mikhail Shapiro

Article

Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

Insert

ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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p2711-pHAGE2-Ef1aL-TAZ4SA-UBC-tagBFP-loxP

Plasmid

#216480

Purpose

lentiviral dual expression of TAZ4SA and tagBFP with loxP site for Cre excision

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Inserts

TAZ4SA (WWTR1 Human)
tagBFP

Use

Cre/Lox and Lentiviral

Tags

Expression

Mutation

S66A, S89A, S117A, S311A

Promoter

EF1aL and UBC

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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p2709-pHAGE2-Ef1aL-YAP5SA-UBC-tagBFP-loxP

Plasmid

#216478

Purpose

lentiviral dual expression of YAP5SA and tagBFP with loxP site for Cre excision

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Inserts

YAP5SA
tagBFP

Use

Cre/Lox and Lentiviral

Tags

Expression

Mutation

S61A, S109A, S127A, S164A, S381A

Promoter

EF1aL and UBC

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE

Plasmid

#122151

Purpose

Expresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

dCas9 fused to SunTag(24x)

Use

Lentiviral

Tags

2xNLS-P2A-BFP and NLS

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP

Plasmid

#188777

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Jonathan Weissman, Marco Jost

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Expression

Mutation

Promoter

EF1a

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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5xUAS:GAP-EGFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

Plasmid

#158652

Purpose

making transgenics expressing a UAS driven eGFP and NTR 2.0

Depositor

Jeff Mumm

Article

Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

Insert

5xUAS:GAP-eGFP-P2A-NfsB_Vv F70A/F108Y;he:BFP

Use

Tol2-based transgenic expression in zebrafish/oth…

Tags

Expression

Mutation

F70A/F108Y

Promoter

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

Plasmid

#158653

Purpose

making transgenics expressing a UAS driven mCherry and NTR 2.0

Depositor

Jeff Mumm

Article

Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

Insert

5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y;he:BFP

Use

Tol2-based transgenic expression in zebrafish/oth…

Tags

Expression

Mutation

F70A/F108Y

Promoter

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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