We narrowed to 3,145 results for: N-EGFP

1. pJEP322-pAAV-FullU6TO-SaCa9sgRNAi(emx1-sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

   Plasmid

   #113699

   Purpose

   Dox-inducible U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1. Followed by an EFS driven GFP-KASH in a separate reading frame.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Inserts

   SaCas9 gRNA Cassete
   GFP
   

   Use

   AAV and CRISPR

   Tags

   KASH

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. C514-E12: attB5r-RU5u-His6-eGFP-nostop-attB1r

   Plasmid

   #162954

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal eGFP-tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   His6-Green fluorescent protein fusion tag with upstream HTLV RU5 5' UTR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXS-IRES-BLAST EGFP-flag-CAD

   Plasmid

   #188118

   Purpose

   Expresses N-terminal EGFP-tagged/C-terminal flag-tagged human CAD in mammalian cells

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   EGFP tag and Flag tag

   Expression

   Mammalian

   Mutation

   TCC -> AGT silent mutations at nt439-441 elimi…

   Promoter

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA5/FRT-Lyn11-mEGFP-cpHalo∆-(GGS)9-FKBP-P2A-Hpep3-(GGS)3-FRB-mScarlett

   Plasmid

   #205703

   Purpose

   CMV driven co-expression of split HaloTag FKBP- and FRB-fusions (Lyn11-EGFP-FKBP-cpHalo∆ and Hpep1-FRB-mScarlett) in mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Lyn11-EGFP-FKBP-cpHalo∆-P2A-Hpep1-FRB-mScarlett
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti_EF1α-NES-Kinprola_on-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

   Plasmid

   #233373

   Purpose

   EF1α driven co-expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP and Cas9 expression through lentivirus transduction

   Depositor

   Kai Johnsson

   Article

   Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

   Inserts

   NES-Kinprola_on-mEGFP
   FLAG-SV40NLS-Cas9-NLS-T2A-BSD
   

   Use

   Lentiviral

   Tags

   FLAG, NES, and mEGFP

   Expression

   Mutation

   Promoter

   EF1α

   Available Since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti_EF1α-NES-Kinprola_PKA_T/A-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

   Plasmid

   #233372

   Purpose

   EF1α driven co-expression of the inactive PKA activity recorder negative control with T/A mutation Kinprola_PKA_T/A fused to mEGFP and Cas9 expression through lentivirus transduction

   Depositor

   Kai Johnsson

   Article

   Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

   Inserts

   NES-Kinprola_PKA_T/A-mEGFP
   FLAG-SV40NLS-Cas9-NLS-T2A-BSD
   

   Use

   Lentiviral

   Tags

   FLAG, NES, and mEGFP

   Expression

   Mutation

   Promoter

   EF1α

   Available Since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJEP323-pAAV-FullH1TO-SaCa9gRNAi(SapI)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA Empty Cassette

   Plasmid

   #113700

   Purpose

   Dox-inducible H1 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in a gRNAs.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Inserts

   SaCas9 gRNA Cassete
   GFP
   

   Use

   AAV and CRISPR

   Tags

   KASH

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-CMV-EGFP-PolB-PAMmut-Hygro

   Plasmid

   #176086

   Purpose

   EGFP fused to the N-terminus of a POLB containing a mutation in the PAM site used by POLBKOg1 & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   PolB (POLB Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Mutation in the PAM site used by PolBKOg1

   Promoter

   CMV

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. MADR pDonor-H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

   Plasmid

   #131461

   Purpose

   MADR donor plasmid for FlpO+Cre-mediated insertion of WT H3F3A and oncogenic PDGFRA D842V and TRP53

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
   

   Use

   Cre/Lox and Synthetic Biology; Mosaic analysis fo…

   Tags

   Trp53-V5, H3F3A-EGFP-AU1

   Expression

   Mammalian

   Mutation

   Pdgfra D842V

   Promoter

   none (4x polyA to mitigate episomal expression)

   Available Since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-UCOE-SFFV-Zim3-dCas9-P2A-EGFP

    Plasmid

    #188899

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS, P2A-GFP, and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    SFFV

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-CMV-EGFP-PolB(K72A)-PAMmut-Hygro

    Plasmid

    #176087

    Purpose

    EGFP fused to the N-terminus of POLB containing the mutation Lys72Ala, a mutation in the PAM site used by POLBKOg1 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Mutation in Lys72 to Ala, and mutation in the PAM…

    Promoter

    CMV

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. MADR pDonor-H3F3A-K27M-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid

    #131462

    Purpose

    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A K27M, PDGFRA D842V, and TRP53

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    H3F3A-K27M-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    

    Use

    Cre/Lox and Synthetic Biology; Mosaic analysis fo…

    Tags

    Trp53-V5, H3F3A-EGFP-AU1

    Expression

    Mammalian

    Mutation

    H3F3A K27M, Pdgfra D842V

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available Since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. MADR pDonor-H3F3A-G34R-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid

    #131463

    Purpose

    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A G34R, PDGFRA D842V, and TRP53

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    H3F3A-G34R-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    

    Use

    Cre/Lox and Synthetic Biology; Mosaic analysis fo…

    Tags

    Trp53-V5, H3F3A-EGFP-AU1

    Expression

    Mammalian

    Mutation

    H3F3A G34R, Pdgfra D842V

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available Since

    July 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV_hSyn-PdCO-EGFP-WPRE (AAV1)

    Viral Prep

    #198513-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV_hSyn-PdCO-EGFP-WPRE (#198513). In addition to the viral particles, you will also receive purified pAAV_hSyn-PdCO-EGFP-WPRE plasmid DNA. Synapsin-driven expression of the optimized opsin PdCO in frame with EGFP. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Promoter

    Syn

    Tags

    EGFP

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

15. pAAV_hSyn-PdCO-EGFP-WPRE (AAV5)

    Viral Prep

    #198513-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV_hSyn-PdCO-EGFP-WPRE (#198513). In addition to the viral particles, you will also receive purified pAAV_hSyn-PdCO-EGFP-WPRE plasmid DNA. Synapsin-driven expression of the optimized opsin PdCO in frame with EGFP. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Promoter

    Syn

    Tags

    EGFP

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

16. pHR-UCOE-EF1a-Zim3-dCas9-loxP-P2A-EGFP-loxP

    Plasmid

    #188773

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHR-UCOE-SFFV-Zim3-dCas9-loxP-P2A-EGFP-loxP

    Plasmid

    #188774

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    SFFV

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV-EGFP-PolB-(K35A/K68A/K72A)-PAMmut-Hygro

    Plasmid

    #176089

    Purpose

    EGFP fused the N-terminus of POLB containing the mutations Lys35Ala, Lys68Ala, and Lys72Ala, a mutation in the PAM site used by POLB gRNA1 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Mutations in Lys35 to Ala, Lys68 to Ala, and Lys7…

    Promoter

    CMV

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro

    Plasmid

    #141395

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(PAMmut)

    Plasmid

    #176139

    Purpose

    EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    XRCC1-T2A-PolB (XRCC1, POLB Human)
    

    Use

    Lentiviral

    Tags

    EGFP and MYC

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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