We narrowed to 2,547 results for: organization Addgene

1. pQE60 R130A wgMDH

   Plasmid

   #204266

   Purpose

   Bacterial expression of R130A wgMDH with IPTG induction - Active site mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   R130A

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQE60 R124A wgMDH

   Plasmid

   #204257

   Purpose

   Bacterial expression of R124A wgMDH with IPTG induction - Active site mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   R124A

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pfa6a mScarlet-FKBP::KANMX

   Plasmid

   #185905

   Purpose

   Plasmid for cloning mScarlet fused to FKBP into yeast.

   Depositor

   Matthew Good

   Article

   Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

   Insert

   mScarlet-FKBP
   

   Tags

   mScarlet fused to FKBP

   Expression

   Yeast

   Promoter

   SP6

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQE60 R196A wgMDH

   Plasmid

   #204271

   Purpose

   Bacterial expression of R196A wgMDH with IPTG induction - Active site mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   R196A

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-Dcp1b

   Plasmid

   #153976

   Purpose

   Fluorescent protein fused to mRNA decapping enzyme 1b

   Depositor

   Gia Voeltz

   Article

   Lee et al Science. 2020 Jan 31;367(6477). pii: 367

   Insert

   mRNA recapping enzybe 1b (DCP1B Human)
   

   Tags

   pAcGFP-C1

   Expression

   Mammalian

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. ZJOM65

   Plasmid

   #133654

   Purpose

   Integration of VPH1-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast vacuole.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-VPH1-mCherry (VPH1 Budding Yeast)
   

   Tags

   mCherry

   Expression

   Yeast

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB_iMYOD1_P2A_GFP_Puro

   Plasmid

   #168804

   Purpose

   PiggyBac transposon construct with doxycycline inducible MYOD1 + GFP expression

   Depositor

   Prashant Mali

   Article

   Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

   Insert

   MYOD1 (MYOD1 Human, Synthetic)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Aug. 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. ZJOM135

   Plasmid

   #133659

   Purpose

   Integration of SEC63-2mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-SEC63-2mTagBFP2 (SEC63 Budding Yeast)
   

   Tags

   mTagBFP2

   Expression

   Yeast

   Available Since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. ZJOM82

   Plasmid

   #133648

   Purpose

   Integration of NAB2-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast nucleus.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-NAB2-mCherry (NAB2 Budding Yeast)
   

   Tags

   mCherry

   Expression

   Yeast

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. ZJOM64

    Plasmid

    #133655

    Purpose

    Integration of COX4-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast mitochondria.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-COX4-DuDre (COX4 Budding Yeast)
    

    Tags

    DuDre

    Expression

    Yeast

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. SNAP-Dcp2

    Plasmid

    #153975

    Purpose

    SNAP-tag polypeptide fused to mRNA decapping enzyme 2

    Depositor

    Gia Voeltz

    Article

    Lee et al Science. 2020 Jan 31;367(6477). pii: 367

    Insert

    mRNA Decapping enzyme 2 (DCP2 Human)
    

    Tags

    SNAP-C1

    Expression

    Mammalian

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET22b_PstS_1

    Plasmid

    #78198

    Purpose

    Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

    Depositor

    Martin Webb

    Article

    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

    Insert

    pstS (A197C)
    

    Expression

    Bacterial

    Mutation

    A197C (please see depositors comment below)

    Promoter

    T7

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pfa6a PhoCl-TsCC(B)::KANMX

    Plasmid

    #185906

    Purpose

    Plasmid for cloning PhoCl fused to TsCC(B) into yeast.

    Depositor

    Matthew Good

    Article

    Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

    Insert

    PhoCl-TsCC(B)
    

    Tags

    PhoCl fluorophore with C-terminal TsCC(B)

    Expression

    Yeast

    Promoter

    SP6

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAVS1 SA-2A-puro-pA_hSYN1_dTomato-SV40pA

    Plasmid

    #196192

    Purpose

    human synapsin promoter driven dTomato expression at human AAVS1 safe harbour locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    2xCHS4_hSYN1_INTRON_dTomato-WPRE-SV40-2xCHS4
    

    Use

    Znf donor

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pfa6a mScarlet-TsCC(B)::KANMX

    Plasmid

    #176397

    Purpose

    Plasmid for cloning mScarlet fused to TsCC(B) coiled coil into yeast.

    Depositor

    Matthew Good

    Article

    Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

    Insert

    mScarlet-TsCC(B)
    

    Tags

    mScarlet fused to TsCC(B)

    Expression

    Bacterial and Yeast

    Promoter

    none

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. SPLICS Po-Mt Short P2A

    Plasmid

    #164116

    Purpose

    Detect the short Peroxisomes-mitochondria contact

    Depositor

    Marisa Brini, Tito Calì

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split GFP Po-Mt Short P2A
    

    Expression

    Mammalian

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET22b_PstS_3

    Plasmid

    #78200

    Purpose

    Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor

    Depositor

    Martin Webb

    Article

    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

    Insert

    pstS (A17C,I76G,A197C)
    

    Expression

    Bacterial

    Mutation

    A17C,I76G,A197C (please see depositor comment bel…

    Promoter

    T7

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.tgoGAP1

    Plasmid

    #78737

    Purpose

    Expression of Ca2+ sensor GAP1 in the trans Golgi apparatus

    Depositor

    Teresa Alonso

    Article

    Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

    Insert

    tgoGAP1
    

    Tags

    N terminal 81 amino acids of human galactosyltran…

    Expression

    Mammalian

    Mutation

    D117A/D119A/D163A in aequorin moiety

    Promoter

    CMV

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pQE60 Q58L wgMDH

    Plasmid

    #204246

    Purpose

    Bacterial expression of Q58L wgMDH with IPTG induction - Subunit interface mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Q58L

    Promoter

    T5

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET_SZ1-RGG-RGG

    Plasmid

    #124942

    Purpose

    Expression of SZ1-RGG-RGG

    Depositor

    Daniel Hammer, Matthew Good, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    SYNZIP1-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Expression

    Bacterial

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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