We narrowed to 4,154 results for: pCMV

1. pCMV4a-SIRT6-G60A-FLAG

   Plasmid

   #102323

   Purpose

   Human SIRT6 G60A with FLAG tag for mammalian expression

   Depositor

   Hening Lin

   Article

   Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

   Insert

   SIRT6 (SIRT6 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed glycine 60 to alanine

   Promoter

   CMV

   Available since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-hA3A-BE3-Y130F

   Plasmid

   #113428

   Purpose

   Expresses hA3A-BE3-Y130F in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   hA3A-BE3-Y130F (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   hAPOBEC3A_Y130F

   Promoter

   CMV

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. 3XFlag pCMV5-TOPO TAZ ∂CC

   Plasmid

   #24816

   Depositor

   Jeff Wrana

   Article

   Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

   Insert

   TAZ (WWTR1 Human)
   

   Use

   Tags

   3XFlag

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 222-265 (coiled coil doma…

   Promoter

   Available since

   May 14, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pCMV-BE4-SsAPOBEC3B R54Q

   Plasmid

   #138344

   Purpose

   Mammalian expression plasmid for BE4-SsAPOBEC3B R54Q

   Depositor

   Nicole Gaudelli

   Article

   Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

   Insert

   BE4-SsAPOBEC3B R54Q
   

   Use

   Synthetic Biology

   Tags

   BP-NLS

   Expression

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-FLAG-MAGE-2-429

   Plasmid

   #89090

   Purpose

   mammalian expression of full-length human MAGE-A11 with N-terminal FLAG tag

   Depositor

   Elizabeth Wilson

   Article

   Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

   Insert

   MAGE-A11 (MAGEA11 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

6. pCMV_UdgX-EE-UdgX-NG-nCas9-UdgX

   Plasmid

   #163572

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-EE-UdgX-NG-nCas9-UdgX
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

   Promoter

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-MtAcKRS (human-opti)

   Plasmid

   #164195

   Purpose

   To express a Methanosarcina thermophila derived AcKRS in mammalian cells for genetic code expansion

   Depositor

   Tao Liu

   Article

   Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

   Insert

   MtAcKRS-478(human-opti)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   D76G; L325M; L329I; Y330F; L333A; C372F

   Promoter

   CMV

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-Tag2-Sox9 T236A

   Plasmid

   #111455

   Purpose

   Transient expression of Flag-tagged human Sox9 with T236A mutation

   Depositor

   Sharon Pine

   Article

   Hong et al Nucleic Acids Res. 2016 Oct 14;44(18):88

   Insert

   Sox9 (SOX9 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   T236A mutation

   Promoter

   CMV

   Available since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. 1247 pCMVb p300 HA delta33

   Plasmid

   #10719

   Depositor

   William Sellers

   Insert

   p300 delta33 (EP300 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Mutant does not bind to E1A. Amino acids 1737 to…

   Promoter

   Available since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pCMV-TTP(C147R)-GG-PUF

    Plasmid

    #52138

    Purpose

    Receiving vector for PUM-HD assembly and expression in mammalian cells

    Depositor

    Huimin Zhao

    Article

    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

    Type

    Empty backbone

    Use

    Tags

    (GGGGS)3GGGG linker, 3xFlag, PUM-HD flanking regi…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV5-Flag-mEPLIN alpha

    Plasmid

    #40955

    Depositor

    Scott Maul, Elizabeth Luna

    Article

    Maul et al Gene. 2001 Jan 10;262(1-2):155-60.

    Insert

    EPLIN alpha (Lima1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pCMV Flag cycT2b (P#1718)

    Plasmid

    #14599

    Depositor

    Matija Peterlin

    Article

    Kurosu et al Gene. 2004 Dec 8. 343(1):173-9.

    Insert

    cycT2 (CCNT2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    A137T compared to NP_490595.1

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-LOx-IRES-EGFP

    Plasmid

    #166877

    Purpose

    Express the bacterial lactate oxidase (LOx) enzyme in mammalian cells

    Depositor

    Sergey Kasparov

    Article

    Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.2396

    Insert

    Lactate oxidase
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV_RBMX-eA3A-UdgX-nCas9

    Plasmid

    #163546

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    RBMX-eA3A-UdgX-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Promoter

    Available since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcmv3-STING-C1471S-FLAG

    Plasmid

    #198188

    Purpose

    Mammalian expression of FLAG-tagged mouse STING1 C1471S

    Depositor

    Yun Zhao

    Article

    Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

    Insert

    TMEM173 (Sting1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    The cysteine in STING at position 147 mutates to …

    Promoter

    CMV

    Available since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-BE4-PpAPOBEC1 H122A

    Plasmid

    #138338

    Purpose

    Mammalian expression plasmid for BE4-PpABOBEC1H122A

    Depositor

    Nicole Gaudelli

    Article

    Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

    Insert

    BE4-PpAPOBEC1 H122A
    

    Use

    Synthetic Biology

    Tags

    BP-NLS

    Expression

    Mutation

    Change of His122 to Ala.

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-PDK1-FLAG-LOV*

    Plasmid

    #202205

    Purpose

    Expresses the photoproximity labeling catalyst LOV* fused to PDK1 in mammalian cells

    Depositor

    Thomas Muir

    Article

    Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

    Insert

    [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial (PDK1 Human)
    

    Use

    Tags

    FLAG and LOV*

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-PEmax-P2A-hP53DD

    Plasmid

    #214084

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-hP53DD

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    PEmax-P2A-hP53DD (TP53 SpCas9 is from Streptococcus pyogenes; MMLV_RT is from the Moloney murine leukemia virus, Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-mTagBFP2-FRB-Rab7

    Plasmid

    #214273

    Purpose

    Mammalian expression of mTagBFP2-FRB-Rab7 (late endosome marker)

    Depositor

    Shinya Tsukiji

    Article

    Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

    Insert

    mTagBFP2-FRB-Rab7A (RAB7A Canis lupus familiaris (Dog))
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV6M-Pak1 H83L H86L

    Plasmid

    #12211

    Depositor

    Jonathan Chernoff

    Article

    Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

    Insert

    Pak1 (PAK1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    PBD (p21-binding domain) mutant: H83L, H86L (see …

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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