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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,798 results for: pcr

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Showing: 1661 - 1680 of 4798 results

  1. pNDGG038

    Plasmid
    #231338
    Purpose
    Terminator; BEVA2.0 T12m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module. Cloned via BpiI GG Assembly of PCR amplicon into POGG006, SpR.
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Insert
    BEVA2.0 T12m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module.
    Expression
    Bacterial
    Available Since
    May 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    231338-plasmid-map-sequence-id-472130

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  2. pNDGG037

    Plasmid
    #231337
    Purpose
    Terminator; BEVA2.0 T2m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module. Cloned via BpiI GG Assembly of PCR amplicon into POGG006, SpR.
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Insert
    BEVA2.0 T2m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module.
    Expression
    Bacterial
    Available Since
    May 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    231337-plasmid-map-sequence-id-472127

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  3. pTE5423_p3WJdB-glcO60

    Plasmid
    #221194
    Purpose
    DNA template of GlcR sensor with glcO60 operator: T7 promoter-glcO60-3WJdB reporter-T7 terminator linear DNA template (PCR amplified with oSB0021+22) is used in the IVT sensor system
    Depositor
    Tobias Erb
    Article
    Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0
    Insert
    T7 promoter-glcO60-3WJdB
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    T7 promoter with downstream glcO60 operator
    Available Since
    Sept. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    221194-plasmid-map-sequence-id-441878

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  10. pENTR-D-kcnj15

    Plasmid
    #173145
    Purpose
    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.
    Depositor
    GuangJun Zhang
    Article
    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.
    Insert
    kcnj15 (kcnj15 Zebrafish)
    Use
    Pcr cloning vector
    Promoter
    No promoter at 5 end; T7 promoter at 3 end.
    Available Since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    173145-plasmid-map-sequence-id-343321

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  12. pDONR221-kcnj3a

    Plasmid
    #173129
    Purpose
    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.
    Depositor
    GuangJun Zhang
    Article
    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.
    Insert
    kcnj3a (kcnj3a Zebrafish)
    Use
    Pcr cloning vector
    Promoter
    No promoter at 5 end; T7 promoter at 3 end.
    Available Since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    173129-plasmid-map-sequence-id-342882

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  13. pDONR221-kcnj5

    Plasmid
    #173132
    Purpose
    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.
    Depositor
    GuangJun Zhang
    Article
    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.
    Insert
    kcnj5 (kcnj5 Zebrafish)
    Use
    Pcr cloning vector
    Promoter
    No promoter at 5 end; T7 promoter at 3 end.
    Available Since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    173132-plasmid-map-sequence-id-343129

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  14. pDONR221-kcnj6

    Plasmid
    #173133
    Purpose
    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.
    Depositor
    GuangJun Zhang
    Article
    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.
    Insert
    kcnj6 (kcnj6 Zebrafish)
    Use
    Pcr cloning vector
    Promoter
    No promoter at 5 end; T7 promoter at 3 end.
    Available Since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    173133-plasmid-map-sequence-id-343132

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  20. BB3cH_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104907
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Jan. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    104907-plasmid-map-sequence-id-206042

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Showing: 1661 - 1680 of 4798 results