Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 10,667 results for: plasmids 101

Showing: 1661 - 1680 of 10667 results

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pTS2392-Tier1-PhCMV-p2a_medFT

Plasmid

#169529

Purpose

Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "medium" maturation medFT fused to a "self-cleaving" peptide p2a (PhCMV-p2a_medFT-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven P2A-fused fluorescent timer, medium maturation time

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pVH269-Tier1-OVanO2-PCMVmin2-SEAP-p2A-iRFP670

Plasmid

#169667

Purpose

Tier-1 vector encoding PVanO2-driven SEAP-p2A-iRFP670 expression (OVanO2-PCMVmin-2-SEAP-p2A-iRFP670-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

vanillic acid-controlled SEAP and iRFP expression

Expression

Mammalian

Promoter

VanO2-PCMVmin

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pVH321-2-Tier1-PhCMV-NLS-dCas9-3xNLS

Plasmid

#169596

Purpose

Tier-1 vector encoding PhCMV-driven NLS-dCas9-3xNLS expression (PhCMV-NLS-dCas9-3xNLS-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

dead S.pyogenes Cas9

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2406-Tier1-PhCMV-NTEV

Plasmid

#169501

Purpose

Tier-1 vector encoding PhCMV-driven N-terminal fragment of a split tabacco etch virus protease splitTEV (PhCMV-N-TEV-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven N-terminal fragment of split tobacco etch virus protease

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-null (BPK1077)

Plasmid

#171128

Purpose

Plasmid encoding a pCMV-T7 with a 2 amino acid peptide prior to a stop codon.

Depositor

Benjamin Kleinstiver

Article

Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

Insert

pCMV-T7 null expression plasmid that encodes a two amino acid peptide (Met-Asp)

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

pCMV and T7

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pJS263

Plasmid

#134141

Purpose

Bacterial expression of C. elegans Ska1 microtubule binding domain (MTBD)

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

Ska1 MTBD-His (Y106G6H.15 Nematode)

Expression

Bacterial

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AAA-nLuc-3XFLAG

Plasmid

#127306

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AAA start codon (Negative control plasmid)

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/GGG-nLuc-3XFLAG

Plasmid

#127307

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from GGG start codon (Negative control plasmid)

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter mir50-7

Plasmid

#46716

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir50-7

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter mir50-5

Plasmid

#46714

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir50-5

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter mir50-4

Plasmid

#46713

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir50-4

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter mir50-3

Plasmid

#46712

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir50-3

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter mir50-2

Plasmid

#46711

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir50-2

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2 mir-1-1 reporter mir50-1

Plasmid

#46710

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir50-1

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2 mir-1-1 reporter mir44-5

Plasmid

#46708

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir44-5

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2 mir-1-1 reporter mir44-3

Plasmid

#46706

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir44-3

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2 mir-1-1 reporter mir44-2

Plasmid

#46705

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir44-2

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2 mir-1-1 reporter mir44-1

Plasmid

#46704

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir44-1

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2 mir-1-1 reporter mir40-7

Plasmid

#46702

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir40-7

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2 mir-1-1 reporter mir40-5

Plasmid

#46700

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir40-5

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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