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NSP4-FR-2Strep

Plasmid

#210316

Purpose

Expresses SARS-CoV-2 NSP4 in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)

Tags

2Strep

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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NSP4-mCherry

Plasmid

#210319

Purpose

Expresses SARS-CoV-2 NSP4 fused with mCherry in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)

Tags

mCherry

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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FKBP10-2Strep

Plasmid

#210357

Purpose

Expresses FKBP10 fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

FKBP10 (FKBP10 Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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DNAJC11-2Strep

Plasmid

#210354

Purpose

Expresses DNAJC11 fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

DNAJC11 (DNAJC11 Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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rUBR5_2383-2442_pGEX6P1

Plasmid

#209791

Purpose

Bacterial expression of UBR5 Mlle domain

Depositor

Kalle Gehring

Article

Lim et al J Biol Chem. 2006 May 19;281(20):14376-8

Insert

Mlle domain of UBR5 (Ubr5 Rat)

Tags

GST

Expression

Bacterial

Mutation

aa 2383-2442 only

Promoter

tac

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgWAVE2-2

Plasmid

#210136

Purpose

knock out WAVE2 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Actin-binding protein WASF2 (WASF2 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgWAVE2-1

Plasmid

#210135

Purpose

knock out WAVE2 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Actin-binding protein WASF2 (WASF2 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-AcGFP-CI

Plasmid

#196849

Purpose

Expression of green fluorescent reporter AcGFP driven by the strong Beta-actin promoter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

AcGFP

Expression

Mammalian

Mutation

None (wt)

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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Interface-1 (a)

Plasmid

#204428

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

R627A K663A R683A

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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Interface-1 (b)

Plasmid

#204429

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

E626A T630A K668A E671A

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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