We narrowed to 5,078 results for: mcs

1. pGL4_EGR1p-BC0132-luc2

   Plasmid

   #227136

   Purpose

   Barcoded assay, MAPK sensor; barcode BC0132

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   EGR1 promoter driving barcode 0132 and a luciferase reporter gene
   

   Expression

   Mammalian

   Available Since

   Nov. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTBL3346 PB-nicking guide-blast

   Plasmid

   #226665

   Purpose

   PiggyBac cargo vector encoding protective nicking sgRNA marked with blasticidin

   Depositor

   Chang Liu

   Article

   Loveless et al bioRxiv 2021.11.05.467507

   Insert

   protective nicking sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX552-EF1a-DIO-mCherry_Dicer gRNA

   Plasmid

   #215279

   Purpose

   Expresses Dicer gRNA in a Cre dependent mcherry vector

   Depositor

   Bryan Copits

   Article

   Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

   Insert

   Dicer control gRNA
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX552-EF1a-DIO-mCherry_DicerTTT gRNA

   Plasmid

   #215280

   Purpose

   Expresses Dicer Control gRNA in a Cre dependent mcherry vector

   Depositor

   Bryan Copits

   Article

   Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

   Insert

   Dicer control gRNA
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTag4C_SP-HTR2A-V2R-NTEV-tcs-GV-2xHA

   Plasmid

   #214615

   Purpose

   HTR2A receptor for split TEV assay

   Depositor

   Michael Wehr

   Article

   Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

   Insert

   HTR2A-NTEV (HTR2A Human)
   

   Tags

   TEV-Gal4-VP16-2xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Hy_pRnrS eGFP SV40

   Plasmid

   #195062

   Purpose

   Expresses eGFP mRNA from the Drosophila RnrS promoter

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   eGFP
   

   Expression

   Insect

   Promoter

   RnrS

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Hy_pAna eGFP SV40

   Plasmid

   #195063

   Purpose

   Expresses eGFP mRNA from the Drosophila Ana promoter

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   eGFP
   

   Expression

   Insect

   Promoter

   Ana

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB3

   Plasmid

   #201534

   Purpose

   Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

   Depositor

   Dan Park

   Article

   Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

   Insert

   ilvA-relE_RBS3_CB3
   

   Use

   Synthetic Biology

   Mutation

   rhaS W190G

   Promoter

   PrhaBAD (rhamnose)

   Available Since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. NK811

   Plasmid

   #196408

   Purpose

   Nanoluc luciferase under S. rosetta SPAG6 promoter with mutated RFX binding site with internal actin-firefly control

   Depositor

   Nicole King

   Article

   Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

   Insert

   Sro5spag6_mut-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
   

   Use

   Luciferase

   Mutation

   N/A (synthetic)

   Available Since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB2

    Plasmid

    #201533

    Purpose

    Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

    Depositor

    Dan Park

    Article

    Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

    Insert

    ilvA-relE_RBS3_CB2
    

    Use

    Synthetic Biology

    Mutation

    rhaR L128+1bp (frameshift), ilvA G455C

    Promoter

    PrhaBAD (rhamnose)

    Available Since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB4

    Plasmid

    #201535

    Purpose

    Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

    Depositor

    Dan Park

    Article

    Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

    Insert

    ilvA-relE_RBS3_CB4
    

    Use

    Synthetic Biology

    Mutation

    rhaS ΔM1-H5, PrhaSR Δ179bp

    Promoter

    PrhaBAD (rhamnose)

    Available Since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. acvr1l-VAAA-HA

    Plasmid

    #201905

    Purpose

    in vitro transcription of RNA encoding HA-tagged Acvr1l that contains 4 amino acid substitutions: T186V, S187A, S189A, S191A

    Depositor

    Mary Mullins

    Article

    Tajer et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

    Insert

    acvr1l (acvr1l Zebrafish)
    

    Tags

    HA

    Mutation

    T186V, S187A, S189A, S191A

    Promoter

    SP6

    Available Since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJL1-AcrA

    Plasmid

    #198214

    Purpose

    pJL1 plasmid encoding wildtype AcrA from C. jejuni containing 2 native internal glycosylation sites recognized by PglB and a C-terminal 6x-His tag

    Depositor

    Michael Jewett

    Article

    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

    Insert

    AcrA
    

    Tags

    6x His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENmRFPCNAL2 pc1054

    Plasmid

    #167567

    Purpose

    The fusion protein contains a SV40 NLS followed by mRFP, linker and human PCNA.

    Depositor

    Cristina Cardoso

    Article

    Sporbert et al Nucleic Acids Res. 2005 Jun 21;33(11):35

    Insert

    Proliferating Cell Nuclear Antigen (PCNA Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. HyWnt3-ΔRep:Luc

    Plasmid

    #192987

    Purpose

    Plasmid to test the Hydra Wnt3 promoter activity (full deletion of the repressor element)

    Depositor

    Brigitte Galliot

    Article

    Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

    Insert

    Hydra Wnt3 promoter
    

    Expression

    Mammalian

    Mutation

    Repressor element deleted

    Available Since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOP004

    Plasmid

    #185985

    Purpose

    Encodes a 0.8 kb bacterial transcription unit with Broccoli-tRNA fluorogenic aptamer shortly upstream of the intrinsic terminator

    Depositor

    Georgi Belogurov

    Article

    Huang et al Methods Enzymol. 2022;675:207-233. doi:

    Insert

    Bacterial transcription unit encoding Broccoli-tRNA fluorogenic aptamer
    

    Expression

    Bacterial

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOP005

    Plasmid

    #186030

    Purpose

    Encodes a 0.8 kb bacterial transcription unit with Broccoli-tRNA fluorogenic aptamer shortly downstream of the promoter

    Depositor

    Georgi Belogurov

    Article

    Huang et al Methods Enzymol. 2022;675:207-233. doi:

    Insert

    Bacterial transcription unit encoding Broccoli-tRNA fluorogenic aptamer
    

    Expression

    Bacterial

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX459-HypaCas9-mR2-CfANLN_sgRNA

    Plasmid

    #183879

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    Canis familiaris ANLN sgRNA spacer
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX459-HypaCas9-H2BC11_sgRNA

    Plasmid

    #183883

    Purpose

    pX459V2.0-HypaCas9 plasmid with H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    H2BC11 sgRNA spacer (H2BC11 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX459-HypaCas9-MYH10_sgRNA

    Plasmid

    #183887

    Purpose

    pX459V2.0-HypaCas9 plasmid with MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    MYH10 sgRNA spacer (MYH10 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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