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Showing: 1701 - 1720 of 1954 results

  1. pUF1-dCas9-Sir2a

    Plasmid
    #122510
    Purpose
    Combining with the specific sgRNA sequence to repress the Plasmodium falciparum endogenous gene transcription, through the decreasing of H3K9 acetylation.
    Depositor
    Lubin Jiang
    Article
    Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116
    Insert
    dCas9-Sir2a
    Use
    CRISPR
    Tags
    3*FLAG
    Expression
    Mutation
    dCas9(D10A,H840A)
    Promoter
    PfHsp86
    Available since
    June 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    122510-plasmid-map-sequence-id-243592

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  2. pTriEX-Antenna-GDI Cdc42

    Plasmid
    #101681
    Purpose
    "Cdc42 was modified with a FRET ‘binding antenna’ that selectively reported Cdc42 binding to endogenous GDI..." -Hodgson et al
    Depositor
    Klaus Hahn
    Article
    Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/
    Insert
    Cdc42
    Use
    Tags
    6xHis and FRET Antenna (mCerulean-cpVenus)
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Promoter
    CMV
    Available since
    March 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    101681-plasmid-map-sequence-id-194353

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  4. LLP779_αGCN4-VP64-CO

    Plasmid
    #211772
    Purpose
    SunTag counterpart binding domain, αGCN4, fused to transcriptional activator VP64, with GFP selection
    Depositor
    Ryan Lister
    Article
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Insert
    aGCN4-VP64
    Use
    Tags
    3xTy1
    Expression
    Mammalian
    Mutation
    Last 300 bp codon-optimised (CO) to detect VP64 e…
    Promoter
    pEF1a and pSV40
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    211772-plasmid-map-sequence-id-417389

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  5. LLP790_αGCN4-EZH2-FL-CO

    Plasmid
    #211784
    Purpose
    SunTag counterpart binding domain, aGCN4, fused to polycomb repressive complex subunit EZH2, with GFP selection
    Depositor
    Ryan Lister
    Article
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Insert
    SnoopCatcher-KRAB
    Use
    Tags
    2xOLLAS
    Expression
    Mammalian
    Mutation
    Last 300 bp codon-optimised (CO) to detect KRAB e…
    Promoter
    pEF1a and pSV40
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    211784-plasmid-map-sequence-id-417361

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  9. pONSY-mCherry

    Plasmid
    #111874
    Purpose
    This plasmid expresses mCherry fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.
    Depositor
    Inaki Ruiz-Trillo
    Article
    Parra-Acero et al Development. 2018 May 11. pii: dev.16210
    Insert
    mCherry
    Use
    Capsaspora owczarzaki
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Elongation Factor 1 alpha (EF1a) from Capsaspora
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    111874-plasmid-map-sequence-id-213482

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  12. pONSY-Venus

    Plasmid
    #111875
    Purpose
    This plasmid expresses Venus fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.
    Depositor
    Inaki Ruiz-Trillo
    Article
    Parra-Acero et al Development. 2018 May 11. pii: dev.16210
    Insert
    Venus
    Use
    Capsaspora owczarzaki
    Tags
    Venus
    Expression
    Mutation
    Promoter
    Elongation Factor 1 alpha (EF1a) from Capsaspora
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    111875-plasmid-map-sequence-id-213485

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  16. GTL-ir

    Plasmid
    #81102
    Purpose
    Gene Tagging vector LEU/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)
    Depositor
    Serge Pelet
    Article
    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231
    Insert
    tdiRFP
    Use
    Cre/Lox
    Tags
    tdiRFP
    Expression
    Yeast
    Mutation
    codon optimized second iRFP to avoid internal rec…
    Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    81102-plasmid-map-sequence-id-154289

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Showing: 1701 - 1720 of 1954 results