We narrowed to 12,265 results for: cel.2

1. pSMT3-FBF2

   Plasmid

   #166972

   Purpose

   Expresses FBF-2 in E. coli for purification and crystallization studies

   Depositor

   Traci Hall

   Article

   Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

   Insert

   FBF-2 (fbf-2 Nematode)
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   FBF-2 PUM RNA binding domain; amino acids 164-575

   Promoter

   T7

   Available Since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5-Myc-PSK2

   Plasmid

   #197117

   Purpose

   Expression of Myc-tagged PSK2 / TAOK1 in mammalian cells

   Depositor

   Jonathan Morris

   Article

   Zihni et al J Biol Chem. 2006 Mar 17;281(11):7317-23

   Insert

   TAOK1 (TAOK1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Wild Type

   Promoter

   SV40

   Available Since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-PML B-box1 WT

   Plasmid

   #185632

   Purpose

   expression of GST-tagged wild-type PML B-box1 domain in bacterial cells

   Depositor

   Irina Bezsonova

   Article

   Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

   Insert

   promyelocytic leukemia protein (PML Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   none

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-PML B-box1 W157E

   Plasmid

   #185641

   Purpose

   expression of GST-tagged mutant PML B-box1 W157E domain in bacterial cells

   Depositor

   Irina Bezsonova

   Article

   Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

   Insert

   promyelocytic leukemia protein (PML Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   W157E

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-PML B-box1 F158E

   Plasmid

   #185642

   Purpose

   expression of GST-tagged mutant PML B-box1 F158E domain in bacterial cells

   Depositor

   Irina Bezsonova

   Article

   Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

   Insert

   promyelocytic leukemia protein (PML Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   F158E

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSNAPf-Cox8A Control Plasmid

   Plasmid

   #101129

   Purpose

   Expression of SNAP-Tagged Cox8A in Mammalian cells

   Depositor

   Ana Egana, New England Biolabs

   Insert

   Cox8A (COX8A Human)
   

   Tags

   SNAP-tag (SNAPf)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pCD86-mEGFP

   Plasmid

   #190745

   Purpose

   Expression of mEGFP-tagged CD86 in mammalian cells.

   Depositor

   Dave Piston

   Article

   Foust et al bioRxiv 2021.11.03.467169

   Insert

   CD86 B7-2, B7.2, B70, CD28LG2, LAB (CD86 Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCLIPf-Cox8A Control Plasmid

   Plasmid

   #101130

   Purpose

   Expression of CLIP-Tagged Cox8A in Mammalian cells

   Depositor

   Ana Egana, New England Biolabs

   Insert

   Cox8A (COX8A Human)
   

   Tags

   CLIP-tag (CLIPf)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pCJ1022

   Plasmid

   #228762

   Purpose

   Plasmid expressing Cas9 and 2 gRNAs for mouse Pkd2. Use for disruption of mouse Pkd2 in cultured cells.

   Depositor

   Bradley Yoder

   Insert

   Pkd2 gRNAs (Pkd2 Mouse)
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-MUC2 D1

    Plasmid

    #181812

    Purpose

    Expresses human MUC2 D1, residues 21-389

    Depositor

    Deborah Fass

    Article

    Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

    Insert

    Muc2 D1 (MUC2 Human)
    

    Tags

    6xHistadine

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCJ1021

    Plasmid

    #228761

    Purpose

    Plasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.

    Depositor

    Bradley Yoder

    Insert

    Ift88 gRNAs (Ift88 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. Mtp-p53-ECFP

    Plasmid

    #53961

    Purpose

    Expresses CFP tagged p53 in mammalian cells

    Depositor

    Uri Alon, Galit Lahav

    Article

    Lahav et al Nat Genet. 2004 Feb;36(2):147-50. Epub 2

    Insert

    p53 (TP53 Human)
    

    Tags

    ECFP

    Expression

    Mammalian

    Promoter

    metallothionein promoter

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCLJ-F2P-LoxP-PGK-NeoR-LoxP

    Plasmid

    #227503

    Purpose

    Inserts 500bp Prdm8 promoter in place of the Fgf5 promoter along with removable selection cassette

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    Prdm8 promoter
    

    Use

    AAV and Cre/Lox

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCLJ-F2P-LoxP-PGK-PuroR-LoxP

    Plasmid

    #227502

    Purpose

    Inserts 500bp Prdm8 promoter in place of the Fgf5 promoter along with removable selection cassette

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    Prdm8 promoter
    

    Use

    AAV and Cre/Lox

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCLJ-P2F-Frt-PGK-Puro-Frt

    Plasmid

    #227501

    Purpose

    Inserts 500bp Fgf5 promoter in place of the Prdm8 promoter along with removable selection cassette

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    Fgf5 promoter
    

    Use

    AAV and Mouse Targeting

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCLJ-Fgf5-t2A-eGFP

    Plasmid

    #227504

    Purpose

    Inserts t2a-eGFP at the 3' end of the Fgf5 gene alsong with a removable selection cassette

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    EGFP
    

    Use

    AAV and Cre/Lox

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Cry2 mCherry GRK2 ct in pcDNA3.1

    Plasmid

    #64212

    Purpose

    mCherry fused between Cry2 and GRK2ct to study cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

    Inserts

    Cry2 PHR (CRY2 Mustard Weed)
    GRK2 ct (GRK2 Human)
    

    Tags

    mcherry

    Expression

    Mammalian

    Promoter

    CMV and cmv

    Available Since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

18. Cry2 mCherry GRK3 ct in pcDNA3.1

    Plasmid

    #64213

    Purpose

    mCherry fused between Cry2 and GRK3 ct to study cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

    Inserts

    Cry2 PHR (CRY2 Mustard Weed)
    GRK3 ct (GRK3 Human)
    

    Tags

    mcherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pCW57.1-Tet-UPF1S124A/N138A/T139A (pRKB268)

    Plasmid

    #99144

    Purpose

    Inducible lentiviral construct to express FLAG-tagged UPF1S124A/N138A/T139A

    Depositor

    Robert Bradley

    Article

    Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

    Insert

    UPF1 (UPF1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    First 127 codons are optimized to reduce GC conte…

    Promoter

    pTight

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28-His-MBP-NLS-RfxCas13d-NLS-HA

    Plasmid

    #171586

    Purpose

    Bacterial expression of NLS-RfxCas13d-NLS-HA

    Depositor

    Neville Sanjana

    Article

    Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

    Insert

    His-MBP-NLS-RfxCas13d-NLS-HA
    

    Tags

    HA

    Expression

    Bacterial

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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